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Article: The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy

TitleThe dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy
基于AFM的化疗药物与质粒DNA间动态相互作用观测
Authors
Issue Date2017
PublisherSpringer Verlag, co-published with Science China Press. The Journal's web site is located at https://link.springer.com/journal/40843
Citation
Science China Materials, 2017, v. 60, p. 269-278 How to Cite?
AbstractThe advent of atomic force microscopy (AFM) provides a powerful tool for imaging individual DNA molecules. Chemotherapy drugs are often related to DNAs. Though many specific drug-DNA interactions have been observed by AFM, knowledge about the dynamic interactions between chemotherapy drugs and plasmid DNAs is still scarce. In this work, AFM was applied to investigate the nanoscale interactions between plasmid DNAs and two commercial chemotherapy drugs (methotrexate and cisplatin). Plasmid DNAs were immobilized on mica which was coated by silanes in advance. AFM imaging distinctly revealed the dynamic changes of single plasmid DNAs after the stimulation of methotrexate and cisplatin. Geometric features of plasmid DNAs were extracted from AFM images and the statistical results showed that the geometric features of plasmid DNAs changed significantly after the stimulation of drugs. This research provides a novel idea to study the actions of chemotherapy drugs against plasmid DNAs at the single-molecule level.
Persistent Identifierhttp://hdl.handle.net/10722/261780
ISSN
2017 Impact Factor: 4.318
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, M-
dc.contributor.authorLiu, L-
dc.contributor.authorXiao, X-
dc.contributor.authorXi, N-
dc.contributor.authorWang, Y-
dc.date.accessioned2018-09-28T04:47:45Z-
dc.date.available2018-09-28T04:47:45Z-
dc.date.issued2017-
dc.identifier.citationScience China Materials, 2017, v. 60, p. 269-278-
dc.identifier.issn2095-8226-
dc.identifier.urihttp://hdl.handle.net/10722/261780-
dc.description.abstractThe advent of atomic force microscopy (AFM) provides a powerful tool for imaging individual DNA molecules. Chemotherapy drugs are often related to DNAs. Though many specific drug-DNA interactions have been observed by AFM, knowledge about the dynamic interactions between chemotherapy drugs and plasmid DNAs is still scarce. In this work, AFM was applied to investigate the nanoscale interactions between plasmid DNAs and two commercial chemotherapy drugs (methotrexate and cisplatin). Plasmid DNAs were immobilized on mica which was coated by silanes in advance. AFM imaging distinctly revealed the dynamic changes of single plasmid DNAs after the stimulation of methotrexate and cisplatin. Geometric features of plasmid DNAs were extracted from AFM images and the statistical results showed that the geometric features of plasmid DNAs changed significantly after the stimulation of drugs. This research provides a novel idea to study the actions of chemotherapy drugs against plasmid DNAs at the single-molecule level.-
dc.languageeng-
dc.publisherSpringer Verlag, co-published with Science China Press. The Journal's web site is located at https://link.springer.com/journal/40843-
dc.relation.ispartofScience China Materials-
dc.rightsThe final publication is available at Springer via http://dx.doi.org/[insert DOI]-
dc.titleThe dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy-
dc.title基于AFM的化疗药物与质粒DNA间动态相互作用观测-
dc.typeArticle-
dc.identifier.emailXi, N: xining@hku.hk-
dc.identifier.authorityXi, N=rp02044-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1007/s40843-016-5152-2-
dc.identifier.hkuros292799-
dc.identifier.volume60-
dc.identifier.spage269-
dc.identifier.epage278-
dc.identifier.isiWOS:000398129000008-
dc.publisher.placeGermany-

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