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Conference Paper: In vitro derivation of oligodendrocyte precursors from neural progenitors harbored in the adult bone marrow - implications for remyelination therapy

TitleIn vitro derivation of oligodendrocyte precursors from neural progenitors harbored in the adult bone marrow - implications for remyelination therapy
Authors
Issue Date2017
PublisherSociety for Neuroscience. The Journal's web site is located at https://www.sfn.org/annual-meeting/past-and-future-annual-meetings
Citation
The 47th Annual Meeting of the Society for Neuroscience (SfN 2017), San Diego, CA., 11-15 November 2017. In Neuroscience 2017 Abstracts, paper no. 475.14 How to Cite?
AbstractLoss of myelin due either to congenital abnormalities or traumatic injuries impacts on conduction of nerve impulses, causing neurological deficits. Our recent success with a strategy that enriches and expands the neural progenitor subpopulation among adult samples of bone marrow stromal cells provided impetus for pursuit of bone marrow-derived neural progenitors (BM-NPs) as source for deriving oligodendrocyte precursors (OPs) for use in transplantation and remyelination. Cultures of rat BM-NPs treated with supplements of β-heregulin, PDGF-AA and bFGF fostered the derivation of oligodendrocyte precursors in 3 weeks. These BM-OPs were positive for the OP markers, NG2, Olig2, PDGFRα and Sox10. The BM-OPs were then subjected to in vitro myelination assay in co-cultures with purified DRG neurons. In 2 weeks, BM-OPs matured into oligodendrocytes and extended myelin basic protein-positive processes along multiple neurites. The BM-OPs were further transplanted into the corpus callosum of myelin-deficient, juvenile Shiverer mice. Mature oligodendrocytes and ultrastructure of compact myelin were identifiable in the corpus callosum in 6 weeks. Our findings indicate BMSCs as a possible source of oligodendrocyte precursors for CNS remyelination therapy.
DescriptionPoster 475. Demyelinating Disorders: Mechanisms and Treatment: Topic: B.13. Demyelinating Disorders - no. 475.14/M10
Persistent Identifierhttp://hdl.handle.net/10722/261273

 

DC FieldValueLanguage
dc.contributor.authorShum, DKY-
dc.contributor.authorTsui, YP-
dc.contributor.authorWu, KLK-
dc.contributor.authorCai, S-
dc.contributor.authorTam, KW-
dc.contributor.authorChan, YS-
dc.date.accessioned2018-09-14T08:55:30Z-
dc.date.available2018-09-14T08:55:30Z-
dc.date.issued2017-
dc.identifier.citationThe 47th Annual Meeting of the Society for Neuroscience (SfN 2017), San Diego, CA., 11-15 November 2017. In Neuroscience 2017 Abstracts, paper no. 475.14-
dc.identifier.urihttp://hdl.handle.net/10722/261273-
dc.descriptionPoster 475. Demyelinating Disorders: Mechanisms and Treatment: Topic: B.13. Demyelinating Disorders - no. 475.14/M10-
dc.description.abstractLoss of myelin due either to congenital abnormalities or traumatic injuries impacts on conduction of nerve impulses, causing neurological deficits. Our recent success with a strategy that enriches and expands the neural progenitor subpopulation among adult samples of bone marrow stromal cells provided impetus for pursuit of bone marrow-derived neural progenitors (BM-NPs) as source for deriving oligodendrocyte precursors (OPs) for use in transplantation and remyelination. Cultures of rat BM-NPs treated with supplements of β-heregulin, PDGF-AA and bFGF fostered the derivation of oligodendrocyte precursors in 3 weeks. These BM-OPs were positive for the OP markers, NG2, Olig2, PDGFRα and Sox10. The BM-OPs were then subjected to in vitro myelination assay in co-cultures with purified DRG neurons. In 2 weeks, BM-OPs matured into oligodendrocytes and extended myelin basic protein-positive processes along multiple neurites. The BM-OPs were further transplanted into the corpus callosum of myelin-deficient, juvenile Shiverer mice. Mature oligodendrocytes and ultrastructure of compact myelin were identifiable in the corpus callosum in 6 weeks. Our findings indicate BMSCs as a possible source of oligodendrocyte precursors for CNS remyelination therapy.-
dc.languageeng-
dc.publisherSociety for Neuroscience. The Journal's web site is located at https://www.sfn.org/annual-meeting/past-and-future-annual-meetings-
dc.relation.ispartofSociety for Neuroscience Abstracts-
dc.rightsSociety for Neuroscience Abstracts. Copyright © Society for Neuroscience.-
dc.titleIn vitro derivation of oligodendrocyte precursors from neural progenitors harbored in the adult bone marrow - implications for remyelination therapy-
dc.typeConference_Paper-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.emailCai, S: caisa@hku.hk-
dc.identifier.emailTam, KW: tamkw@hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.authorityShum, DKY=rp00321-
dc.identifier.authorityChan, YS=rp00318-
dc.identifier.hkuros291268-
dc.identifier.spageno. 475.14-
dc.identifier.epageno. 475.14-
dc.publisher.placeUnited States-

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