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Article: Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells

TitleMiddle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells
Authors
Issue Date2018
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal of Biological Chemistry, 2018, v. 293 n. 30, p. 11709-11726 How to Cite?
AbstractCoronavirus tropism is predominantly determined by the interaction between coronavirus spikes and the host receptors. In this regard, coronaviruses have evolved a complicated receptor-recognition system through their spike proteins. Spikes from highly related coronaviruses can recognize distinct receptors, whereas spikes of distant coronaviruses can employ the same cell-surface molecule for entry. Moreover, coronavirus spikes can recognize a broad range of cell-surface molecules in addition to the receptors and thereby can augment coronavirus attachment or entry. The receptor of Middle East respiratory syndrome coronavirus (MERS-CoV) is dipeptidyl peptidase 4 (DPP4). In this study, we identified membrane-associated 78-kDa glucose-regulated protein (GRP78) as an additional binding target of the MERS-CoV spike. Further analyses indicated that GRP78 could not independently render nonpermissive cells susceptible to MERS-CoV infection but could facilitate MERS-CoV entry into permissive cells by augmenting virus attachment. More importantly, by exploring potential interactions between GRP78 and spikes of other coronaviruses, we discovered that the highly conserved human GRP78 could interact with the spike protein of bat coronavirus HKU9 (bCoV-HKU9) and facilitate its attachment to the host cell surface. Taken together, our study has identified GRP78 as a host factor that can interact with the spike proteins of two Betacoronaviruses, the lineage C MERS-CoV and the lineage D bCoV-HKU9. The capacity of GRP78 to facilitate surface attachment of both a human coronavirus and a phylogenetically related bat coronavirus exemplifies the need for continuous surveillance of the evolution of animal coronaviruses to monitor their potential for human adaptations.
Persistent Identifierhttp://hdl.handle.net/10722/259069
ISSN
2017 Impact Factor: 4.011
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChu, H-
dc.contributor.authorChan, CM-
dc.contributor.authorZhang, X-
dc.contributor.authorWang, Y-
dc.contributor.authorYuan, S-
dc.contributor.authorZhou, J-
dc.contributor.authorAu Yeung, KHR-
dc.contributor.authorSze, KH-
dc.contributor.authorYang, D-
dc.contributor.authorShuai, HV-
dc.contributor.authorHou, Y-
dc.contributor.authorLi, C-
dc.contributor.authorZhao, X-
dc.contributor.authorPoon, KM-
dc.contributor.authorLeung, ASP-
dc.contributor.authorYeung, ML-
dc.contributor.authorYan, J-
dc.contributor.authorLu, G-
dc.contributor.authorJin, D-
dc.contributor.authorGao, GF-
dc.contributor.authorChan, JFW-
dc.contributor.authorYuen, KY-
dc.date.accessioned2018-09-03T04:01:02Z-
dc.date.available2018-09-03T04:01:02Z-
dc.date.issued2018-
dc.identifier.citationJournal of Biological Chemistry, 2018, v. 293 n. 30, p. 11709-11726-
dc.identifier.issn0021-9258-
dc.identifier.urihttp://hdl.handle.net/10722/259069-
dc.description.abstractCoronavirus tropism is predominantly determined by the interaction between coronavirus spikes and the host receptors. In this regard, coronaviruses have evolved a complicated receptor-recognition system through their spike proteins. Spikes from highly related coronaviruses can recognize distinct receptors, whereas spikes of distant coronaviruses can employ the same cell-surface molecule for entry. Moreover, coronavirus spikes can recognize a broad range of cell-surface molecules in addition to the receptors and thereby can augment coronavirus attachment or entry. The receptor of Middle East respiratory syndrome coronavirus (MERS-CoV) is dipeptidyl peptidase 4 (DPP4). In this study, we identified membrane-associated 78-kDa glucose-regulated protein (GRP78) as an additional binding target of the MERS-CoV spike. Further analyses indicated that GRP78 could not independently render nonpermissive cells susceptible to MERS-CoV infection but could facilitate MERS-CoV entry into permissive cells by augmenting virus attachment. More importantly, by exploring potential interactions between GRP78 and spikes of other coronaviruses, we discovered that the highly conserved human GRP78 could interact with the spike protein of bat coronavirus HKU9 (bCoV-HKU9) and facilitate its attachment to the host cell surface. Taken together, our study has identified GRP78 as a host factor that can interact with the spike proteins of two Betacoronaviruses, the lineage C MERS-CoV and the lineage D bCoV-HKU9. The capacity of GRP78 to facilitate surface attachment of both a human coronavirus and a phylogenetically related bat coronavirus exemplifies the need for continuous surveillance of the evolution of animal coronaviruses to monitor their potential for human adaptations.-
dc.languageeng-
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/-
dc.relation.ispartofJournal of Biological Chemistry-
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.-
dc.rightsThis research was originally published in [Journal Name]. Author(s). Title. Journal Name. Year. Vol:pp-pp. © the American Society for Biochemistry and Molecular Biology-
dc.titleMiddle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells-
dc.typeArticle-
dc.identifier.emailChu, H: hinchu@hku.hk-
dc.identifier.emailChan, CM: cmchan@hku.hk-
dc.identifier.emailYuan, S: yuansf@hku.hk-
dc.identifier.emailZhou, J: jiezhou@hku.hk-
dc.identifier.emailAu Yeung, KHR: rex.auyeung@hku.hk-
dc.identifier.emailSze, KH: khsze@hku.hk-
dc.identifier.emailShuai, HV: shuaihp@hku.hk-
dc.identifier.emailHou, Y: houhou@hku.hk-
dc.identifier.emailLi, C: licun@hku.hk-
dc.identifier.emailPoon, KM: vinpoon@hku.hk-
dc.identifier.emailLeung, ASP: aspleung@hkucc.hku.hk-
dc.identifier.emailYeung, ML: pmlyeung@hku.hk-
dc.identifier.emailJin, D: dyjin@hku.hk-
dc.identifier.emailChan, JFW: jfwchan@hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.authorityChu, H=rp02125-
dc.identifier.authorityZhou, J=rp01412-
dc.identifier.authorityAu Yeung, KHR=rp01877-
dc.identifier.authoritySze, KH=rp00785-
dc.identifier.authorityYeung, ML=rp01402-
dc.identifier.authorityJin, D=rp00452-
dc.identifier.authorityChan, JFW=rp01736-
dc.identifier.authorityYuen, KY=rp00366-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1074/jbc.RA118.001897-
dc.identifier.scopuseid_2-s2.0-85050779327-
dc.identifier.hkuros288904-
dc.identifier.volume293-
dc.identifier.issue30-
dc.identifier.spage11709-
dc.identifier.epage11726-
dc.identifier.isiWOS:000440134500005-
dc.publisher.placeUnited States-

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