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Article: Cross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia

TitleCross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia
Authors
KeywordsDromedary camel
ELISA
MERS
Real time-PCR
Saudi Arabia
Issue Date2018
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/716388/description#description
Citation
Journal of Infection and Public Health, 2018, v. 11 n. 3, p. 331-338 How to Cite?
AbstractBACKGROUND: Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the Middle East. The epidemiological profiles of the MERS-CoV infections suggest zoonotic transmission from an animal reservoir to humans. METHODS: This study was designed to investigate animal herds associated with Middle East respiratory syndrome (MERS)-infected patients in Saudi Arabia, during the last three years (2014-2016). Nasal swabs and serum samples from 584 dromedary camels, 39 sheep, 51 goats, and 2 cattle were collected. Nasal samples from camels, sheep, goats, and cattle were examined by real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV RNA, and the Anti-MERS ELISA assay was performed to detect camel humeral immune response (IgG) to MERS-CoV S1 antigen infection. The complete genome sequencing of ten MERS-CoV camel isolates and phylogenetic analysis was performed. RESULTS: The data indicated that seventy-five dromedary camels were positive for MERS-CoV RNA; the virus was not detected in sheep, goats, and cattle. MERS-CoV RNA from infected camels was not detected beyond 2 weeks after the first positive result was detected in nasal swabs obtained from infected camels. Anti-MERS ELISA assays showed that 70.9% of camels related to human cases had antibodies to MERS-CoV. The full genome sequences of the ten MERS-CoV camel isolates were identical to their corresponding patients and were grouped together within the larger MERS-CoV sequences cluster for human and camel isolates reported form the Arabian Peninsula. CONCLUSIONS: These findings indicate that camels are a significant reservoir for the maintenance of MERS-CoVs, and they are an important source of human infection with MERS.
Persistent Identifierhttp://hdl.handle.net/10722/258392
ISSN
2021 Impact Factor: 7.537
2020 SCImago Journal Rankings: 0.983
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKasem, S-
dc.contributor.authorQasim, I-
dc.contributor.authorAl-Hufofi, A-
dc.contributor.authorHashim, O-
dc.contributor.authorAlkarar, A-
dc.contributor.authorAbu-Obeida, A-
dc.contributor.authorGaafer, A-
dc.contributor.authorElfadil, A-
dc.contributor.authorZaki, A-
dc.contributor.authorAl-Romaihi, A-
dc.contributor.authorBabekr, N-
dc.contributor.authorEl-Harby, N-
dc.contributor.authorHussien, R-
dc.contributor.authorAL-Sahaf, A-
dc.contributor.authorAl-Doweriej, A-
dc.contributor.authorBayoumi, F-
dc.contributor.authorPoon, LML-
dc.contributor.authorChu, KW-
dc.contributor.authorPeiris, JSM-
dc.contributor.authorPerera, RAPM-
dc.date.accessioned2018-08-22T01:37:44Z-
dc.date.available2018-08-22T01:37:44Z-
dc.date.issued2018-
dc.identifier.citationJournal of Infection and Public Health, 2018, v. 11 n. 3, p. 331-338-
dc.identifier.issn1876-0341-
dc.identifier.urihttp://hdl.handle.net/10722/258392-
dc.description.abstractBACKGROUND: Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the Middle East. The epidemiological profiles of the MERS-CoV infections suggest zoonotic transmission from an animal reservoir to humans. METHODS: This study was designed to investigate animal herds associated with Middle East respiratory syndrome (MERS)-infected patients in Saudi Arabia, during the last three years (2014-2016). Nasal swabs and serum samples from 584 dromedary camels, 39 sheep, 51 goats, and 2 cattle were collected. Nasal samples from camels, sheep, goats, and cattle were examined by real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV RNA, and the Anti-MERS ELISA assay was performed to detect camel humeral immune response (IgG) to MERS-CoV S1 antigen infection. The complete genome sequencing of ten MERS-CoV camel isolates and phylogenetic analysis was performed. RESULTS: The data indicated that seventy-five dromedary camels were positive for MERS-CoV RNA; the virus was not detected in sheep, goats, and cattle. MERS-CoV RNA from infected camels was not detected beyond 2 weeks after the first positive result was detected in nasal swabs obtained from infected camels. Anti-MERS ELISA assays showed that 70.9% of camels related to human cases had antibodies to MERS-CoV. The full genome sequences of the ten MERS-CoV camel isolates were identical to their corresponding patients and were grouped together within the larger MERS-CoV sequences cluster for human and camel isolates reported form the Arabian Peninsula. CONCLUSIONS: These findings indicate that camels are a significant reservoir for the maintenance of MERS-CoVs, and they are an important source of human infection with MERS.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/716388/description#description-
dc.relation.ispartofJournal of Infection and Public Health-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectDromedary camel-
dc.subjectELISA-
dc.subjectMERS-
dc.subjectReal time-PCR-
dc.subjectSaudi Arabia-
dc.titleCross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia-
dc.typeArticle-
dc.identifier.emailPoon, LML: llmpoon@hkucc.hku.hk-
dc.identifier.emailChu, KW: dkwchu@hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.emailPerera, RAPM: mahenp@hku.hk-
dc.identifier.authorityPoon, LML=rp00484-
dc.identifier.authorityPeiris, JSM=rp00410-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.jiph.2017.09.022-
dc.identifier.pmid28993171-
dc.identifier.scopuseid_2-s2.0-85030789295-
dc.identifier.hkuros286580-
dc.identifier.volume11-
dc.identifier.issue3-
dc.identifier.spage331-
dc.identifier.epage338-
dc.identifier.isiWOS:000432468400008-
dc.publisher.placeNetherlands-
dc.identifier.issnl1876-0341-

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