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Article: Cross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia
Title | Cross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia |
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Authors | |
Keywords | Dromedary camel ELISA MERS Real time-PCR Saudi Arabia |
Issue Date | 2018 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/716388/description#description |
Citation | Journal of Infection and Public Health, 2018, v. 11 n. 3, p. 331-338 How to Cite? |
Abstract | BACKGROUND: Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the Middle East. The epidemiological profiles of the MERS-CoV infections suggest zoonotic transmission from an animal reservoir to humans. METHODS: This study was designed to investigate animal herds associated with Middle East respiratory syndrome (MERS)-infected patients in Saudi Arabia, during the last three years (2014-2016). Nasal swabs and serum samples from 584 dromedary camels, 39 sheep, 51 goats, and 2 cattle were collected. Nasal samples from camels, sheep, goats, and cattle were examined by real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV RNA, and the Anti-MERS ELISA assay was performed to detect camel humeral immune response (IgG) to MERS-CoV S1 antigen infection. The complete genome sequencing of ten MERS-CoV camel isolates and phylogenetic analysis was performed. RESULTS: The data indicated that seventy-five dromedary camels were positive for MERS-CoV RNA; the virus was not detected in sheep, goats, and cattle. MERS-CoV RNA from infected camels was not detected beyond 2 weeks after the first positive result was detected in nasal swabs obtained from infected camels. Anti-MERS ELISA assays showed that 70.9% of camels related to human cases had antibodies to MERS-CoV. The full genome sequences of the ten MERS-CoV camel isolates were identical to their corresponding patients and were grouped together within the larger MERS-CoV sequences cluster for human and camel isolates reported form the Arabian Peninsula. CONCLUSIONS: These findings indicate that camels are a significant reservoir for the maintenance of MERS-CoVs, and they are an important source of human infection with MERS. |
Persistent Identifier | http://hdl.handle.net/10722/258392 |
ISSN | 2021 Impact Factor: 7.537 2020 SCImago Journal Rankings: 0.983 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Kasem, S | - |
dc.contributor.author | Qasim, I | - |
dc.contributor.author | Al-Hufofi, A | - |
dc.contributor.author | Hashim, O | - |
dc.contributor.author | Alkarar, A | - |
dc.contributor.author | Abu-Obeida, A | - |
dc.contributor.author | Gaafer, A | - |
dc.contributor.author | Elfadil, A | - |
dc.contributor.author | Zaki, A | - |
dc.contributor.author | Al-Romaihi, A | - |
dc.contributor.author | Babekr, N | - |
dc.contributor.author | El-Harby, N | - |
dc.contributor.author | Hussien, R | - |
dc.contributor.author | AL-Sahaf, A | - |
dc.contributor.author | Al-Doweriej, A | - |
dc.contributor.author | Bayoumi, F | - |
dc.contributor.author | Poon, LML | - |
dc.contributor.author | Chu, KW | - |
dc.contributor.author | Peiris, JSM | - |
dc.contributor.author | Perera, RAPM | - |
dc.date.accessioned | 2018-08-22T01:37:44Z | - |
dc.date.available | 2018-08-22T01:37:44Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Journal of Infection and Public Health, 2018, v. 11 n. 3, p. 331-338 | - |
dc.identifier.issn | 1876-0341 | - |
dc.identifier.uri | http://hdl.handle.net/10722/258392 | - |
dc.description.abstract | BACKGROUND: Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the Middle East. The epidemiological profiles of the MERS-CoV infections suggest zoonotic transmission from an animal reservoir to humans. METHODS: This study was designed to investigate animal herds associated with Middle East respiratory syndrome (MERS)-infected patients in Saudi Arabia, during the last three years (2014-2016). Nasal swabs and serum samples from 584 dromedary camels, 39 sheep, 51 goats, and 2 cattle were collected. Nasal samples from camels, sheep, goats, and cattle were examined by real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV RNA, and the Anti-MERS ELISA assay was performed to detect camel humeral immune response (IgG) to MERS-CoV S1 antigen infection. The complete genome sequencing of ten MERS-CoV camel isolates and phylogenetic analysis was performed. RESULTS: The data indicated that seventy-five dromedary camels were positive for MERS-CoV RNA; the virus was not detected in sheep, goats, and cattle. MERS-CoV RNA from infected camels was not detected beyond 2 weeks after the first positive result was detected in nasal swabs obtained from infected camels. Anti-MERS ELISA assays showed that 70.9% of camels related to human cases had antibodies to MERS-CoV. The full genome sequences of the ten MERS-CoV camel isolates were identical to their corresponding patients and were grouped together within the larger MERS-CoV sequences cluster for human and camel isolates reported form the Arabian Peninsula. CONCLUSIONS: These findings indicate that camels are a significant reservoir for the maintenance of MERS-CoVs, and they are an important source of human infection with MERS. | - |
dc.language | eng | - |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/716388/description#description | - |
dc.relation.ispartof | Journal of Infection and Public Health | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Dromedary camel | - |
dc.subject | ELISA | - |
dc.subject | MERS | - |
dc.subject | Real time-PCR | - |
dc.subject | Saudi Arabia | - |
dc.title | Cross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia | - |
dc.type | Article | - |
dc.identifier.email | Poon, LML: llmpoon@hkucc.hku.hk | - |
dc.identifier.email | Chu, KW: dkwchu@hku.hk | - |
dc.identifier.email | Peiris, JSM: malik@hkucc.hku.hk | - |
dc.identifier.email | Perera, RAPM: mahenp@hku.hk | - |
dc.identifier.authority | Poon, LML=rp00484 | - |
dc.identifier.authority | Peiris, JSM=rp00410 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1016/j.jiph.2017.09.022 | - |
dc.identifier.pmid | 28993171 | - |
dc.identifier.scopus | eid_2-s2.0-85030789295 | - |
dc.identifier.hkuros | 286580 | - |
dc.identifier.volume | 11 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 331 | - |
dc.identifier.epage | 338 | - |
dc.identifier.isi | WOS:000432468400008 | - |
dc.publisher.place | Netherlands | - |
dc.identifier.issnl | 1876-0341 | - |