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Article: Axial plane optical microscopy

TitleAxial plane optical microscopy
Authors
Issue Date2014
Citation
Scientific Reports, 2014, v. 4 How to Cite?
AbstractWe present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More importantly, APOM uses only a single lens near the sample to achieve selective-plane illumination microscopy, as we demonstrated by three-dimensional (3D) imaging of fluorescent pollens and brain slices. This technique allows fast, high-contrast, and convenient 3D imaging of structures that are hundreds of microns beneath the surfaces of large biological tissues.
Persistent Identifierhttp://hdl.handle.net/10722/256708
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, Tongcang-
dc.contributor.authorOta, Sadao-
dc.contributor.authorKim, Jeongmin-
dc.contributor.authorWong, Zi Jing-
dc.contributor.authorWang, Yuan-
dc.contributor.authorYin, Xiaobo-
dc.contributor.authorZhang, Xiang-
dc.date.accessioned2018-07-24T08:57:40Z-
dc.date.available2018-07-24T08:57:40Z-
dc.date.issued2014-
dc.identifier.citationScientific Reports, 2014, v. 4-
dc.identifier.urihttp://hdl.handle.net/10722/256708-
dc.description.abstractWe present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More importantly, APOM uses only a single lens near the sample to achieve selective-plane illumination microscopy, as we demonstrated by three-dimensional (3D) imaging of fluorescent pollens and brain slices. This technique allows fast, high-contrast, and convenient 3D imaging of structures that are hundreds of microns beneath the surfaces of large biological tissues.-
dc.languageeng-
dc.relation.ispartofScientific Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleAxial plane optical microscopy-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/srep07253-
dc.identifier.pmid25434770-
dc.identifier.scopuseid_2-s2.0-84922671795-
dc.identifier.volume4-
dc.identifier.spagenull-
dc.identifier.epagenull-
dc.identifier.eissn2045-2322-
dc.identifier.isiWOS:000346257700001-

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