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- Publisher Website: 10.1002/anie.201506686
- Scopus: eid_2-s2.0-84954423752
- PMID: 26447926
- WOS: WOS:000367722500015
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Article: Rational Structure-Based Design of Bright GFP-Based Complexes with Tunable Dimerization
Title | Rational Structure-Based Design of Bright GFP-Based Complexes with Tunable Dimerization |
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Authors | |
Keywords | green fluorescent protein protein engineering single-molecule studies dimerization fluorescent probes |
Issue Date | 2015 |
Citation | Angewandte Chemie - International Edition, 2015, v. 54, n. 47, p. 13952-13956 How to Cite? |
Abstract | © 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Fluorescent proteins are transformative tools; thus, any brightness increase is a welcome improvement. We invented the "vGFP strategy" based on structural analysis of GFP bound to a single-domain antibody, predicting tunable dimerization, enhanced brightness (ca. 50 %), and improved pH resistance. We verified all of these predictions using biochemistry, crystallography, and single-molecule studies. We applied the vsfGFP proteins in three diverse scenarios: single-step immunofluorescence in vitro (3× brighter due to dimerization); expression in bacteria and human cells in vivo (1.5× brighter); and protein fusions showing better pH resistance in human cells in vivo. The vGFP strategy thus allows upgrading of existing applications, is applicable to other fluorescent proteins, and suggests a method for tuning dimerization of arbitrary proteins and optimizing protein properties in general. |
Persistent Identifier | http://hdl.handle.net/10722/253175 |
ISSN | 2021 Impact Factor: 16.823 2020 SCImago Journal Rankings: 5.831 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Eshaghi, Majid | - |
dc.contributor.author | Sun, Guangyu | - |
dc.contributor.author | Grüter, Andreas | - |
dc.contributor.author | Lim, Chiew Ling | - |
dc.contributor.author | Chee, Yuemin Celina | - |
dc.contributor.author | Jung, Gregor | - |
dc.contributor.author | Jauch, Ralf | - |
dc.contributor.author | Wohland, Thorsten | - |
dc.contributor.author | Chen, Swaine L. | - |
dc.date.accessioned | 2018-05-11T05:38:48Z | - |
dc.date.available | 2018-05-11T05:38:48Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Angewandte Chemie - International Edition, 2015, v. 54, n. 47, p. 13952-13956 | - |
dc.identifier.issn | 1433-7851 | - |
dc.identifier.uri | http://hdl.handle.net/10722/253175 | - |
dc.description.abstract | © 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Fluorescent proteins are transformative tools; thus, any brightness increase is a welcome improvement. We invented the "vGFP strategy" based on structural analysis of GFP bound to a single-domain antibody, predicting tunable dimerization, enhanced brightness (ca. 50 %), and improved pH resistance. We verified all of these predictions using biochemistry, crystallography, and single-molecule studies. We applied the vsfGFP proteins in three diverse scenarios: single-step immunofluorescence in vitro (3× brighter due to dimerization); expression in bacteria and human cells in vivo (1.5× brighter); and protein fusions showing better pH resistance in human cells in vivo. The vGFP strategy thus allows upgrading of existing applications, is applicable to other fluorescent proteins, and suggests a method for tuning dimerization of arbitrary proteins and optimizing protein properties in general. | - |
dc.language | eng | - |
dc.relation.ispartof | Angewandte Chemie - International Edition | - |
dc.subject | green fluorescent protein | - |
dc.subject | protein engineering | - |
dc.subject | single-molecule studies | - |
dc.subject | dimerization | - |
dc.subject | fluorescent probes | - |
dc.title | Rational Structure-Based Design of Bright GFP-Based Complexes with Tunable Dimerization | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/anie.201506686 | - |
dc.identifier.pmid | 26447926 | - |
dc.identifier.scopus | eid_2-s2.0-84954423752 | - |
dc.identifier.volume | 54 | - |
dc.identifier.issue | 47 | - |
dc.identifier.spage | 13952 | - |
dc.identifier.epage | 13956 | - |
dc.identifier.eissn | 1521-3773 | - |
dc.identifier.isi | WOS:000367722500015 | - |
dc.identifier.issnl | 1433-7851 | - |