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Article: CRISPR/Cas9 Genome-Editing System in Human Stem Cells: Current Status and Future Prospects

TitleCRISPR/Cas9 Genome-Editing System in Human Stem Cells: Current Status and Future Prospects
Authors
Keywordsapplications
challenges
CRISPR/Cas9
human stem cells
prospects
Issue Date2017
PublisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://www.cell.com/molecular-therapy-family/nucleic-acids/latest-content
Citation
Molecular Therapy - Nucleic Acids, 2017, v. 9, p. 230-241 How to Cite?
AbstractGenome-editing involves the insertion, deletion, or replacement of DNA in the genome of a living organism using “molecular scissors.” Traditional genome editing with engineered nucleases for human stem cells is limited by its low efficiency, high cost, and poor specificity. The CRISPR system has recently emerged as a powerful gene manipulation technique with advantages of high editing efficiency and low cost. Although this technique offers huge potential for gene manipulation in various organisms ranging from prokaryotes to higher mammals, there remain many challenges in human stem cell research. In this review, we highlight the basic biology and application of the CRISPR/Cas9 system in current human stem cell research, discuss its advantages and challenges, and debate the future prospects for human stem cells in regenerative medicine.
Persistent Identifierhttp://hdl.handle.net/10722/251862
ISSN
2017 Impact Factor: 5.66
2015 SCImago Journal Rankings: 2.320
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, Z-
dc.contributor.authorZhang, Y-
dc.contributor.authorGao, F-
dc.contributor.authorHan, S-
dc.contributor.authorCheah, KSE-
dc.contributor.authorTse, HF-
dc.contributor.authorLian, Q-
dc.date.accessioned2018-03-21T07:38:26Z-
dc.date.available2018-03-21T07:38:26Z-
dc.date.issued2017-
dc.identifier.citationMolecular Therapy - Nucleic Acids, 2017, v. 9, p. 230-241-
dc.identifier.issn2162-2531-
dc.identifier.urihttp://hdl.handle.net/10722/251862-
dc.description.abstractGenome-editing involves the insertion, deletion, or replacement of DNA in the genome of a living organism using “molecular scissors.” Traditional genome editing with engineered nucleases for human stem cells is limited by its low efficiency, high cost, and poor specificity. The CRISPR system has recently emerged as a powerful gene manipulation technique with advantages of high editing efficiency and low cost. Although this technique offers huge potential for gene manipulation in various organisms ranging from prokaryotes to higher mammals, there remain many challenges in human stem cell research. In this review, we highlight the basic biology and application of the CRISPR/Cas9 system in current human stem cell research, discuss its advantages and challenges, and debate the future prospects for human stem cells in regenerative medicine.-
dc.languageeng-
dc.publisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://www.cell.com/molecular-therapy-family/nucleic-acids/latest-content-
dc.relation.ispartofMolecular Therapy - Nucleic Acids-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectapplications-
dc.subjectchallenges-
dc.subjectCRISPR/Cas9-
dc.subjecthuman stem cells-
dc.subjectprospects-
dc.titleCRISPR/Cas9 Genome-Editing System in Human Stem Cells: Current Status and Future Prospects-
dc.typeArticle-
dc.identifier.emailCheah, KSE: hrmbdkc@hku.hk-
dc.identifier.emailTse, HF: hftse@hkucc.hku.hk-
dc.identifier.emailLian, Q: qzlian@hkucc.hku.hk-
dc.identifier.authorityCheah, KSE=rp00342-
dc.identifier.authorityTse, HF=rp00428-
dc.identifier.authorityLian, Q=rp00267-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.omtn.2017.09.009-
dc.identifier.scopuseid_2-s2.0-85041857919-
dc.identifier.hkuros293014-
dc.identifier.volume9-
dc.identifier.spage230-
dc.identifier.epage241-
dc.identifier.isiWOS:000418494000022-
dc.publisher.placeUnited Kingdom-

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