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postgraduate thesis: Detection of RHCE and JK alleles by sequence-specific polymerase chain reaction

TitleDetection of RHCE and JK alleles by sequence-specific polymerase chain reaction
Authors
Issue Date2017
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
Citation
Man, W. [文詠然]. (2017). Detection of RHCE and JK alleles by sequence-specific polymerase chain reaction. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR.
AbstractGenotyping of RHCE and JK alleles shall essentially supplement the serological typing when the transfusion recipient has been multiply transfused with red cells or the recipient’s red cells are heavily coated with autoantibody. The RHCE and JK alleles are mostly indicated because the corresponding distributions of the antithetical antigens are usually heterogeneous among different ethnic populations. This project was aimed to design a fast, simple and direct PCR-SSP protocol for the RHCE and JK genotyping in the local population. Eighty samples, which covers the most common phenotypes of the Rh(CE) and Kidd blood groups, were included in the study. Ten sets of allele specific primers were designed for sequence-specific polymerase chain reaction (PCR-SSP) under same PCR condition in 7 tubes. The PCR-SSP results showed no discrepancy with the serologic phenotypes. The designed PCR-SSP protocol for RHCE and JK genotyping was then evaluated with 7 transfused patients to test for its clinical application. The PCR-SSP results also showed full agreement with the pre-transfusion phenotypes. The PCR-SSP for RHCE and JK alleles is confirmed to be a quick, cost-economic and accurate strategy that can resolve doubtful serological typings. The implication of Rh(CE) and Kidd antigens shall confirm patients’ phenotypes and assure safe transfusion.
DegreeMaster of Medical Sciences
SubjectPolymerase chain reaction
Blood groups
Blood cells
Blood - Transfusion
Dept/ProgramPathology
Persistent Identifierhttp://hdl.handle.net/10722/251357

 

DC FieldValueLanguage
dc.contributor.authorMan, Wing-yin-
dc.contributor.author文詠然-
dc.date.accessioned2018-02-27T09:53:46Z-
dc.date.available2018-02-27T09:53:46Z-
dc.date.issued2017-
dc.identifier.citationMan, W. [文詠然]. (2017). Detection of RHCE and JK alleles by sequence-specific polymerase chain reaction. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR.-
dc.identifier.urihttp://hdl.handle.net/10722/251357-
dc.description.abstractGenotyping of RHCE and JK alleles shall essentially supplement the serological typing when the transfusion recipient has been multiply transfused with red cells or the recipient’s red cells are heavily coated with autoantibody. The RHCE and JK alleles are mostly indicated because the corresponding distributions of the antithetical antigens are usually heterogeneous among different ethnic populations. This project was aimed to design a fast, simple and direct PCR-SSP protocol for the RHCE and JK genotyping in the local population. Eighty samples, which covers the most common phenotypes of the Rh(CE) and Kidd blood groups, were included in the study. Ten sets of allele specific primers were designed for sequence-specific polymerase chain reaction (PCR-SSP) under same PCR condition in 7 tubes. The PCR-SSP results showed no discrepancy with the serologic phenotypes. The designed PCR-SSP protocol for RHCE and JK genotyping was then evaluated with 7 transfused patients to test for its clinical application. The PCR-SSP results also showed full agreement with the pre-transfusion phenotypes. The PCR-SSP for RHCE and JK alleles is confirmed to be a quick, cost-economic and accurate strategy that can resolve doubtful serological typings. The implication of Rh(CE) and Kidd antigens shall confirm patients’ phenotypes and assure safe transfusion. -
dc.languageeng-
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)-
dc.relation.ispartofHKU Theses Online (HKUTO)-
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subject.lcshPolymerase chain reaction-
dc.subject.lcshBlood groups-
dc.subject.lcshBlood cells-
dc.subject.lcshBlood - Transfusion-
dc.titleDetection of RHCE and JK alleles by sequence-specific polymerase chain reaction-
dc.typePG_Thesis-
dc.description.thesisnameMaster of Medical Sciences-
dc.description.thesislevelMaster-
dc.description.thesisdisciplinePathology-
dc.description.naturepublished_or_final_version-
dc.date.hkucongregation2017-
dc.identifier.mmsid991043983791403414-

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