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Article: Pro-angiogenic effects of Ilexsaponin A1 on human umbilical vein endothelial cells in vitro and zebrafish in vivo

TitlePro-angiogenic effects of Ilexsaponin A1 on human umbilical vein endothelial cells in vitro and zebrafish in vivo
Authors
KeywordsEndothelial cells
Ilex pubescens
Ilexsaponin A1
Pro-angiogenesis
Zebrafish
Issue Date2017
PublisherElsevier GmbH - Urban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed
Citation
Phytomedicine, 2017, v. 36, p. 229-237 How to Cite?
AbstractBackground Ilexsaponin A1 is the major bioactive ingredient of Ilex pubescens Hook. et Arn. This plant has been conventionally used in Traditional Chinese Medicine for the treatment of cardiovascular diseases including stroke, coronary arterial disease, and peripheral vascular diseases. Purpose To investigate the pro-angiogenic effect of Ilexsaponin A1 and its mechanism of action. Study design Human umbilical vein endothelial cells (HUVECs) and transgenic zebrafish Tg(fli1:EGFP) were employed as an in vitro and in vivo model respectively. Methods Pro-angiogenic effects of Ilexsaponin A1 were examined by assessing endothelial cell proliferation, migration, invasion and tube formation. The mechanism of pro-angiogenic effects was investigated by measuring the expression level of various signalling proteins. Furthermore, vascular endothelial growth factor (VEGF) tyrosine kinase inhibitor II (VRI)-induced vascular insufficient transgenic zebrafish model was used to confirm the results of the HUVECs results in vivo. Results Ilexsaponin A1 significantly promoted cell proliferation, migration, invasion and tube formation in HUVECs, and rescued blood vessel loss in VRI-induced vascular insufficient zebrafish. Ilexsaponin A1 upregulated p-Akt, p-mTOR, p-Src, p-FAK, p-MEK, and p-Erk1/2 in HUVECs. Conclusion This study showed that Ilexsaponin A1 exhibits pro-angiogenic activity in HUVECs and VRI-induced vascular insufficient zebrafish, probably by activating Akt/mTOR, MAPK/ERK and Src- and FAK-dependent signalling pathways. The findings suggest that Ilexsaponin A1 and probably I. pubescens, a major source of Ilexsaponin A1, could be developed as a potential therapeutic agent for preventing or treating cardiovascular diseases and/or other diseases related to vascular insufficiency. © 2017 Elsevier GmbH
Persistent Identifierhttp://hdl.handle.net/10722/250021
ISSN
2017 Impact Factor: 3.61
2015 SCImago Journal Rankings: 1.013
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, J-
dc.contributor.authorZhang, J-
dc.contributor.authorZou, L-
dc.contributor.authorLee, SMY-
dc.contributor.authorYang, C-
dc.contributor.authorSeto, SW-
dc.contributor.authorLeung, GPH-
dc.date.accessioned2017-12-20T09:19:31Z-
dc.date.available2017-12-20T09:19:31Z-
dc.date.issued2017-
dc.identifier.citationPhytomedicine, 2017, v. 36, p. 229-237-
dc.identifier.issn0944-7113-
dc.identifier.urihttp://hdl.handle.net/10722/250021-
dc.description.abstractBackground Ilexsaponin A1 is the major bioactive ingredient of Ilex pubescens Hook. et Arn. This plant has been conventionally used in Traditional Chinese Medicine for the treatment of cardiovascular diseases including stroke, coronary arterial disease, and peripheral vascular diseases. Purpose To investigate the pro-angiogenic effect of Ilexsaponin A1 and its mechanism of action. Study design Human umbilical vein endothelial cells (HUVECs) and transgenic zebrafish Tg(fli1:EGFP) were employed as an in vitro and in vivo model respectively. Methods Pro-angiogenic effects of Ilexsaponin A1 were examined by assessing endothelial cell proliferation, migration, invasion and tube formation. The mechanism of pro-angiogenic effects was investigated by measuring the expression level of various signalling proteins. Furthermore, vascular endothelial growth factor (VEGF) tyrosine kinase inhibitor II (VRI)-induced vascular insufficient transgenic zebrafish model was used to confirm the results of the HUVECs results in vivo. Results Ilexsaponin A1 significantly promoted cell proliferation, migration, invasion and tube formation in HUVECs, and rescued blood vessel loss in VRI-induced vascular insufficient zebrafish. Ilexsaponin A1 upregulated p-Akt, p-mTOR, p-Src, p-FAK, p-MEK, and p-Erk1/2 in HUVECs. Conclusion This study showed that Ilexsaponin A1 exhibits pro-angiogenic activity in HUVECs and VRI-induced vascular insufficient zebrafish, probably by activating Akt/mTOR, MAPK/ERK and Src- and FAK-dependent signalling pathways. The findings suggest that Ilexsaponin A1 and probably I. pubescens, a major source of Ilexsaponin A1, could be developed as a potential therapeutic agent for preventing or treating cardiovascular diseases and/or other diseases related to vascular insufficiency. © 2017 Elsevier GmbH-
dc.languageeng-
dc.publisherElsevier GmbH - Urban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed-
dc.relation.ispartofPhytomedicine-
dc.rightsPosting accepted manuscript (postprint): © <year>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.subjectEndothelial cells-
dc.subjectIlex pubescens-
dc.subjectIlexsaponin A1-
dc.subjectPro-angiogenesis-
dc.subjectZebrafish-
dc.titlePro-angiogenic effects of Ilexsaponin A1 on human umbilical vein endothelial cells in vitro and zebrafish in vivo-
dc.typeArticle-
dc.identifier.emailLeung, GPH: gphleung@hkucc.hku.hk-
dc.identifier.authorityLeung, GPH=rp00234-
dc.identifier.doi10.1016/j.phymed.2017.10.006-
dc.identifier.scopuseid_2-s2.0-85032346640-
dc.identifier.hkuros283875-
dc.identifier.volume36-
dc.identifier.spage229-
dc.identifier.epage237-
dc.identifier.isiWOS:000415693500028-
dc.publisher.placeGermany-

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