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Article: Quantitative evaluation of the immunodeficiency of a mouse strain by tumor engraftments

TitleQuantitative evaluation of the immunodeficiency of a mouse strain by tumor engraftments
Authors
KeywordsTumor
Immunodeficiency
Allograft
Xenograft
Leukemia
Issue Date2015
Citation
Journal of Hematology and Oncology, 2015, v. 8, n. 1 How to Cite?
Abstract© 2015 Ye et al. Background: The mouse is an organism that is widely used as a mammalian model for studying human physiology or disease, and the development of immunodeficient mice has provided a valuable tool for basic and applied human disease research. Following the development of large-scale mouse knockout programs and genome-editing tools, it has become increasingly efficient to generate genetically modified mouse strains with immunodeficiency. However, due to the lack of a standardized system for evaluating the immuno-capacity that prevents tumor progression in mice, an objective choice of the appropriate immunodeficient mouse strains to be used for tumor engrafting experiments is difficult. Methods: In this study, we developed a tumor engraftment index (TEI) to quantify the immunodeficiency response to hematologic malignant cells and solid tumor cells of six immunodeficient mouse strains and C57BL/6 wild-type mouse (WT). Results: Mice with a more severely impaired immune system attained a higher TEI score. We then validated that the NOD-scid-IL2Rg-/- (NSI) mice, which had the highest TEI score, were more suitable for xenograft and allograft experiments using multiple functional assays. Conclusions: The TEI score was effectively able to reflect the immunodeficiency of a mouse strain.
Persistent Identifierhttp://hdl.handle.net/10722/249151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYe, Wei-
dc.contributor.authorJiang, Zhiwu-
dc.contributor.authorLi, Guan Xiong-
dc.contributor.authorXiao, Yiren-
dc.contributor.authorLin, Simiao-
dc.contributor.authorLai, Yunxin-
dc.contributor.authorWang, Suna-
dc.contributor.authorLi, Baiheng-
dc.contributor.authorJia, Bei-
dc.contributor.authorLi, Yin-
dc.contributor.authorHuang, Zhi Liang-
dc.contributor.authorLi, Jin-
dc.contributor.authorFeng, Fenglan-
dc.contributor.authorLi, Shuhua-
dc.contributor.authorYao, Huihui-
dc.contributor.authorLiu, Zixia-
dc.contributor.authorCao, Su-
dc.contributor.authorXu, Lin-
dc.contributor.authorLi, Yangqiu-
dc.contributor.authorWu, Donghai-
dc.contributor.authorZeng, Lingwen-
dc.contributor.authorZhong, Mei-
dc.contributor.authorLiu, Pentao-
dc.contributor.authorWen, Zhe Sheng-
dc.contributor.authorXu, Bing-
dc.contributor.authorYao, Yao-
dc.contributor.authorPei, Duanqing-
dc.contributor.authorLi, Peng-
dc.date.accessioned2017-10-27T05:59:14Z-
dc.date.available2017-10-27T05:59:14Z-
dc.date.issued2015-
dc.identifier.citationJournal of Hematology and Oncology, 2015, v. 8, n. 1-
dc.identifier.urihttp://hdl.handle.net/10722/249151-
dc.description.abstract© 2015 Ye et al. Background: The mouse is an organism that is widely used as a mammalian model for studying human physiology or disease, and the development of immunodeficient mice has provided a valuable tool for basic and applied human disease research. Following the development of large-scale mouse knockout programs and genome-editing tools, it has become increasingly efficient to generate genetically modified mouse strains with immunodeficiency. However, due to the lack of a standardized system for evaluating the immuno-capacity that prevents tumor progression in mice, an objective choice of the appropriate immunodeficient mouse strains to be used for tumor engrafting experiments is difficult. Methods: In this study, we developed a tumor engraftment index (TEI) to quantify the immunodeficiency response to hematologic malignant cells and solid tumor cells of six immunodeficient mouse strains and C57BL/6 wild-type mouse (WT). Results: Mice with a more severely impaired immune system attained a higher TEI score. We then validated that the NOD-scid-IL2Rg-/- (NSI) mice, which had the highest TEI score, were more suitable for xenograft and allograft experiments using multiple functional assays. Conclusions: The TEI score was effectively able to reflect the immunodeficiency of a mouse strain.-
dc.languageeng-
dc.relation.ispartofJournal of Hematology and Oncology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectTumor-
dc.subjectImmunodeficiency-
dc.subjectAllograft-
dc.subjectXenograft-
dc.subjectLeukemia-
dc.titleQuantitative evaluation of the immunodeficiency of a mouse strain by tumor engraftments-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/s13045-015-0156-y-
dc.identifier.pmid26022250-
dc.identifier.scopuseid_2-s2.0-84934906688-
dc.identifier.volume8-
dc.identifier.issue1-
dc.identifier.spagenull-
dc.identifier.epagenull-
dc.identifier.eissn1756-8722-
dc.identifier.isiWOS:000356772000001-

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