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Article: ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells

TitleANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells
Authors
Issue Date2015
Citation
Haematologica, 2015, v. 100, n. 5, p. 585-594 How to Cite?
Abstract© 2015 Ferrata Storti Foundation. Successful expansion of hematopoietic stem cells would benefit the use of hematopoietic stem cell transplants in the clinic. Several angiopoietin-like proteins, including angiopoietin-like 7, can support the activity of hematopoietic stem cells. However, effects of ANGPTL7 on human hematopoietic stem cells and the downstream signaling cascade activated by ANGPTL7 are poorly understood. Here, we established a human hematopoietic stem and progenitor cell-supportive mouse fetal liver cell line that specifically expressed the Angptl7 protein. Furthermore, we found ANGPTL7 is capable of stimulating human hematopoietic stem and progenitor cell expansion and increasing the repopulation activities of human hematopoietic progenitors in xenografts. RNA-sequencing analysis showed that ANGPTL7 activated the expression of CXCR4, HOXB4 and Wnt downstream targets in human hematopoietic progenitors. In addition, chemical manipulation of Wnt signaling diminished the effects of ANGPTL7 on human hematopoietic stem and progenitor cells in culture. In summary, we identify the secreted growth factor ANGPTL7 as a regulator of both human hematopoietic stem and progenitor cell expansion and regeneration.
Persistent Identifierhttp://hdl.handle.net/10722/249150
ISSN
2017 Impact Factor: 9.09
2015 SCImago Journal Rankings: 3.010
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorXiao, Yiren-
dc.contributor.authorJiang, Zhiwu-
dc.contributor.authorLi, Yin-
dc.contributor.authorYe, Wei-
dc.contributor.authorJia, Bei-
dc.contributor.authorZhang, Minjie-
dc.contributor.authorXu, Yan-
dc.contributor.authorWu, Donghai-
dc.contributor.authorLai, Liangxue-
dc.contributor.authorChen, Yaoyu-
dc.contributor.authorChang, Yingjun-
dc.contributor.authorHuang, Xiaojun-
dc.contributor.authorLiu, Hudan-
dc.contributor.authorQing, Guoliang-
dc.contributor.authorLiu, Pentao-
dc.contributor.authorLi, Yangqiu-
dc.contributor.authorXu, Bing-
dc.contributor.authorZhong, Mei-
dc.contributor.authorYao, Yao-
dc.contributor.authorPei, Duanqing-
dc.contributor.authorLi, Peng-
dc.date.accessioned2017-10-27T05:59:14Z-
dc.date.available2017-10-27T05:59:14Z-
dc.date.issued2015-
dc.identifier.citationHaematologica, 2015, v. 100, n. 5, p. 585-594-
dc.identifier.issn0390-6078-
dc.identifier.urihttp://hdl.handle.net/10722/249150-
dc.description.abstract© 2015 Ferrata Storti Foundation. Successful expansion of hematopoietic stem cells would benefit the use of hematopoietic stem cell transplants in the clinic. Several angiopoietin-like proteins, including angiopoietin-like 7, can support the activity of hematopoietic stem cells. However, effects of ANGPTL7 on human hematopoietic stem cells and the downstream signaling cascade activated by ANGPTL7 are poorly understood. Here, we established a human hematopoietic stem and progenitor cell-supportive mouse fetal liver cell line that specifically expressed the Angptl7 protein. Furthermore, we found ANGPTL7 is capable of stimulating human hematopoietic stem and progenitor cell expansion and increasing the repopulation activities of human hematopoietic progenitors in xenografts. RNA-sequencing analysis showed that ANGPTL7 activated the expression of CXCR4, HOXB4 and Wnt downstream targets in human hematopoietic progenitors. In addition, chemical manipulation of Wnt signaling diminished the effects of ANGPTL7 on human hematopoietic stem and progenitor cells in culture. In summary, we identify the secreted growth factor ANGPTL7 as a regulator of both human hematopoietic stem and progenitor cell expansion and regeneration.-
dc.languageeng-
dc.relation.ispartofHaematologica-
dc.titleANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells-
dc.typeArticle-
dc.description.natureLink_to_OA_fulltext-
dc.identifier.doi10.3324/haematol.2014.118612-
dc.identifier.pmid25637050-
dc.identifier.scopuseid_2-s2.0-84929165873-
dc.identifier.volume100-
dc.identifier.issue5-
dc.identifier.spage585-
dc.identifier.epage594-
dc.identifier.eissn1592-8721-
dc.identifier.isiWOS:000354788000023-

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