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Article: Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog 1

TitleRapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog 1
Authors
KeywordsRAREoct
Embryonic stem cell
SH-iPSC
PiggyBac transposition
Issue Date2011
Citation
Proceedings of the National Academy of Sciences of the United States of America, 2011, v. 108, n. 45, p. 18283-18288 How to Cite?
AbstractSomatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by expressing four transcription factors: Oct4, Sox2, Klf4, and c-Myc. Here we report that enhancing RA signaling by expressing RA receptors (RARs) or by RA agonists profoundly promoted reprogramming, but inhibiting it using a RAR-α dominant- negative form completely blocked it. Coexpressing Rarg (RAR-γ) and Lrh-1 (liver receptor homologue 1; Nr5a2) with the four factors greatly accelerated reprogramming so that reprogramming of mouse embryonic fibroblast cells to ground-state iPSCs requires only 4 d induction of these six factors. The six-factor combination readily reprogrammed primary human neonatal and adult fibroblast cells to exogenous factor-independent iPSCs, which resembled ground-state mouse ES cells in growth properties, gene expression, and signaling dependency. Our findings demonstrate that signaling through RARs has critical roles in molecular reprogramming and that the synergistic interaction between Rarg and Lrh1 directs reprogramming toward ground-state pluripotency. The human iPSCs described here should facilitate functional analysis of the human genome.
Persistent Identifierhttp://hdl.handle.net/10722/249055
ISSN
2017 Impact Factor: 9.504
2015 SCImago Journal Rankings: 6.883
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Wei-
dc.contributor.authorYang, Jian-
dc.contributor.authorLiu, Hui-
dc.contributor.authorLu, Dong-
dc.contributor.authorChen, Xiongfeng-
dc.contributor.authorZenonos, Zenon-
dc.contributor.authorCampos, Lia S.-
dc.contributor.authorRad, Roland-
dc.contributor.authorGuo, Ge-
dc.contributor.authorZhang, Shujun-
dc.contributor.authorBradley, Allan-
dc.contributor.authorLiu, Pentao-
dc.date.accessioned2017-10-27T05:58:59Z-
dc.date.available2017-10-27T05:58:59Z-
dc.date.issued2011-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2011, v. 108, n. 45, p. 18283-18288-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/249055-
dc.description.abstractSomatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by expressing four transcription factors: Oct4, Sox2, Klf4, and c-Myc. Here we report that enhancing RA signaling by expressing RA receptors (RARs) or by RA agonists profoundly promoted reprogramming, but inhibiting it using a RAR-α dominant- negative form completely blocked it. Coexpressing Rarg (RAR-γ) and Lrh-1 (liver receptor homologue 1; Nr5a2) with the four factors greatly accelerated reprogramming so that reprogramming of mouse embryonic fibroblast cells to ground-state iPSCs requires only 4 d induction of these six factors. The six-factor combination readily reprogrammed primary human neonatal and adult fibroblast cells to exogenous factor-independent iPSCs, which resembled ground-state mouse ES cells in growth properties, gene expression, and signaling dependency. Our findings demonstrate that signaling through RARs has critical roles in molecular reprogramming and that the synergistic interaction between Rarg and Lrh1 directs reprogramming toward ground-state pluripotency. The human iPSCs described here should facilitate functional analysis of the human genome.-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.subjectRAREoct-
dc.subjectEmbryonic stem cell-
dc.subjectSH-iPSC-
dc.subjectPiggyBac transposition-
dc.titleRapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog 1-
dc.typeArticle-
dc.description.natureLink_to_OA_fulltext-
dc.identifier.doi10.1073/pnas.1100893108-
dc.identifier.pmid21990348-
dc.identifier.scopuseid_2-s2.0-81055141362-
dc.identifier.volume108-
dc.identifier.issue45-
dc.identifier.spage18283-
dc.identifier.epage18288-
dc.identifier.eissn1091-6490-
dc.identifier.isiWOS:000296700000031-
dc.identifier.f100013371021-

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