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Article: Chromosomal transposition of PiggyBac in mouse embryonic stem cells

TitleChromosomal transposition of PiggyBac in mouse embryonic stem cells
Authors
KeywordsSleeping beauty
Transposon
Es cell
Issue Date2008
Citation
Proceedings of the National Academy of Sciences of the United States of America, 2008, v. 105, n. 27, p. 9290-9295 How to Cite?
AbstractTransposon systems are widely used for generating mutations in various model organisms. PiggyBac (PB) has recently been shown to transpose efficiently in the mouse germ line and other mammalian cell lines. To facilitate PB's application in mammalian genetics, we characterized the properties of the PB transposon in mouse embryonic stem (ES) cells. We first measured the transposition efficiencies of PB transposon in mouse embryonic stem cells. We next constructed a PB/SB hybrid transposon to compare PB and Sleeping Beauty (SB) transposon systems and demonstrated that PB transposition was inhibited by DNA methylation. The excision and reintegration rates of a single PB from two independent genomic loci were measured and its ability to mutate genes with gene trap cassettes was tested. We examined PB's integration site distribution in the mouse genome and found that PB transposition exhibited local hopping. The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics. © 2008 by The National Academy of Sciences of the USA.
Persistent Identifierhttp://hdl.handle.net/10722/249024
ISSN
2017 Impact Factor: 9.504
2015 SCImago Journal Rankings: 6.883
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Wei-
dc.contributor.authorLin, Chengyi-
dc.contributor.authorLu, Dong-
dc.contributor.authorNing, Zeming-
dc.contributor.authorCox, Tony-
dc.contributor.authorMelvin, David-
dc.contributor.authorWang, Xiaozhong-
dc.contributor.authorBradley, Allan-
dc.contributor.authorLiu, Pentao-
dc.date.accessioned2017-10-27T05:58:54Z-
dc.date.available2017-10-27T05:58:54Z-
dc.date.issued2008-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2008, v. 105, n. 27, p. 9290-9295-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/249024-
dc.description.abstractTransposon systems are widely used for generating mutations in various model organisms. PiggyBac (PB) has recently been shown to transpose efficiently in the mouse germ line and other mammalian cell lines. To facilitate PB's application in mammalian genetics, we characterized the properties of the PB transposon in mouse embryonic stem (ES) cells. We first measured the transposition efficiencies of PB transposon in mouse embryonic stem cells. We next constructed a PB/SB hybrid transposon to compare PB and Sleeping Beauty (SB) transposon systems and demonstrated that PB transposition was inhibited by DNA methylation. The excision and reintegration rates of a single PB from two independent genomic loci were measured and its ability to mutate genes with gene trap cassettes was tested. We examined PB's integration site distribution in the mouse genome and found that PB transposition exhibited local hopping. The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics. © 2008 by The National Academy of Sciences of the USA.-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.subjectSleeping beauty-
dc.subjectTransposon-
dc.subjectEs cell-
dc.titleChromosomal transposition of PiggyBac in mouse embryonic stem cells-
dc.typeArticle-
dc.description.natureLink_to_OA_fulltext-
dc.identifier.doi10.1073/pnas.0801017105-
dc.identifier.pmid18579772-
dc.identifier.scopuseid_2-s2.0-48249130858-
dc.identifier.volume105-
dc.identifier.issue27-
dc.identifier.spage9290-
dc.identifier.epage9295-
dc.identifier.eissn1091-6490-
dc.identifier.isiWOS:000257645400030-

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