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Article: Efficient Cre-loxP-induced mitotic recombination in mouse embryonic stem cells

TitleEfficient Cre-loxP-induced mitotic recombination in mouse embryonic stem cells
Authors
Issue Date2002
Citation
Nature Genetics, 2002, v. 30, n. 1, p. 66-72 How to Cite?
AbstractFLP/FRT-induced mitotic recombination provides a powerful method for creating genetic mosaics in Drosophila and for discerning the function of recessive genes in a heterozygous individual. Here we show that mitotic recombination can be reproducibly induced in mouse embryonic stem (ES) cells, by Cre/loxP technology, at frequencies ranging from 4.2 × 10 -5 (Snrpn) to 7.0 × 10 -3 (D7Mit178) for single allelic loxP sites, and to 5.0 × 10 -2 (D7Mit178) for multiple allelic lox sites, after transient Cre expression. Notably, much of the recombination occurs in G2 and is followed by X segregation, where the recombinant chromatids segregate away from each other during mitosis. It is X segregation that is useful for genetic mosaic analysis because it produces clones of homozygous mutant daughter cells from heterozygous mothers. Our studies confirm the predictions made from studies in Drosophila 1 that suggest that X segregation will not be limited to organisms with strong mitotic pairing, because the forces (sister-chromatid cohesion) responsible for X segregation are an elemental feature of mitosis in all eukaryotes. Our studies also show that genetic mosaic analysis in mice is feasible, at least for certain chromosomal regions.
Persistent Identifierhttp://hdl.handle.net/10722/249001
ISSN
2015 Impact Factor: 31.616
2015 SCImago Journal Rankings: 23.762

 

DC FieldValueLanguage
dc.contributor.authorLiu, Pentao-
dc.contributor.authorJenkins, Nancy A.-
dc.contributor.authorCopeland, Neal G.-
dc.date.accessioned2017-10-27T05:58:50Z-
dc.date.available2017-10-27T05:58:50Z-
dc.date.issued2002-
dc.identifier.citationNature Genetics, 2002, v. 30, n. 1, p. 66-72-
dc.identifier.issn1061-4036-
dc.identifier.urihttp://hdl.handle.net/10722/249001-
dc.description.abstractFLP/FRT-induced mitotic recombination provides a powerful method for creating genetic mosaics in Drosophila and for discerning the function of recessive genes in a heterozygous individual. Here we show that mitotic recombination can be reproducibly induced in mouse embryonic stem (ES) cells, by Cre/loxP technology, at frequencies ranging from 4.2 × 10 -5 (Snrpn) to 7.0 × 10 -3 (D7Mit178) for single allelic loxP sites, and to 5.0 × 10 -2 (D7Mit178) for multiple allelic lox sites, after transient Cre expression. Notably, much of the recombination occurs in G2 and is followed by X segregation, where the recombinant chromatids segregate away from each other during mitosis. It is X segregation that is useful for genetic mosaic analysis because it produces clones of homozygous mutant daughter cells from heterozygous mothers. Our studies confirm the predictions made from studies in Drosophila 1 that suggest that X segregation will not be limited to organisms with strong mitotic pairing, because the forces (sister-chromatid cohesion) responsible for X segregation are an elemental feature of mitosis in all eukaryotes. Our studies also show that genetic mosaic analysis in mice is feasible, at least for certain chromosomal regions.-
dc.languageeng-
dc.relation.ispartofNature Genetics-
dc.titleEfficient Cre-loxP-induced mitotic recombination in mouse embryonic stem cells-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1038/ng788-
dc.identifier.pmid11740496-
dc.identifier.scopuseid_2-s2.0-0036338128-
dc.identifier.volume30-
dc.identifier.issue1-
dc.identifier.spage66-
dc.identifier.epage72-
dc.identifier.f10001003169-

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