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Article: Genetic screens using the piggyBac transposon

TitleGenetic screens using the piggyBac transposon
Authors
KeywordsTransposition
Cancer
Mouse
Transposon
Mutagenesis
PiggyBac
Issue Date2011
Citation
Methods, 2011, v. 53, n. 4, p. 366-371 How to Cite?
AbstractTransposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments. © 2010 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/248982
ISSN
2015 Impact Factor: 3.503
2015 SCImago Journal Rankings: 2.377

 

DC FieldValueLanguage
dc.contributor.authorChew, Su Kit-
dc.contributor.authorRad, Roland-
dc.contributor.authorFutreal, P. Andrew-
dc.contributor.authorBradley, Allan-
dc.contributor.authorLiu, Pentao-
dc.date.accessioned2017-10-27T05:58:47Z-
dc.date.available2017-10-27T05:58:47Z-
dc.date.issued2011-
dc.identifier.citationMethods, 2011, v. 53, n. 4, p. 366-371-
dc.identifier.issn1046-2023-
dc.identifier.urihttp://hdl.handle.net/10722/248982-
dc.description.abstractTransposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments. © 2010 Elsevier Inc.-
dc.languageeng-
dc.relation.ispartofMethods-
dc.subjectTransposition-
dc.subjectCancer-
dc.subjectMouse-
dc.subjectTransposon-
dc.subjectMutagenesis-
dc.subjectPiggyBac-
dc.titleGenetic screens using the piggyBac transposon-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ymeth.2010.12.022-
dc.identifier.pmid21185377-
dc.identifier.scopuseid_2-s2.0-79952707077-
dc.identifier.volume53-
dc.identifier.issue4-
dc.identifier.spage366-
dc.identifier.epage371-
dc.identifier.eissn1095-9130-

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