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Article: Polyphyletic origin of MERS coronaviruses and isolation of a novel clade A strain from dromedary camels in the United Arab Emirates

TitlePolyphyletic origin of MERS coronaviruses and isolation of a novel clade A strain from dromedary camels in the United Arab Emirates
Authors
Issue Date2016
PublisherNature Publishing Group for Shanghai Shangyixun Cultural Communication Company. The Journal's web site is located at http://www.nature.com/emi/marketing/index.html
Citation
Emerging Microbes & Infections, 2016, v. 5, article no. e128 How to Cite?
AbstractLittle is known regarding the molecular epidemiology of Middle East respiratory syndrome coronavirus (MERS-CoV) circulating in dromedaries outside Saudi Arabia. To address this knowledge gap, we sequenced 10 complete genomes of MERS-CoVs isolated from 2 live and 8 dead dromedaries from different regions in the United Arab Emirates (UAE). Phylogenetic analysis revealed one novel clade A strain, the first detected in the UAE, and nine clade B strains. Strain D998/15 had a distinct phylogenetic position within clade A, being more closely related to the dromedary isolate NRCE-HKU205 from Egypt than to the human isolates EMC/2012 and Jordan-N3/2012. A comparison of predicted protein sequences also demonstrated the existence of two clade A lineages with unique amino acid substitutions, A1 (EMC/2012 and Jordan-N3/2012) and A2 (D998/15 and NRCE-HKU205), circulating in humans and camels, respectively. The nine clade B isolates belong to three distinct lineages: B1, B3 and B5. Two B3 strains, D1271/15 and D1189.1/15, showed evidence of recombination between lineages B4 and B5 in ORF1ab. Molecular clock analysis dated the time of the most recent common ancestor (tMRCA) of clade A to March 2011 and that of clade B to November 2011. Our data support a polyphyletic origin of MERS-CoV in dromedaries and the co-circulation of diverse MERS-CoVs including recombinant strains in the UAE.
Persistent Identifierhttp://hdl.handle.net/10722/245139
ISSN
2018 Impact Factor: 6.212
2015 SCImago Journal Rankings: 1.774
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLau, SKP-
dc.contributor.authorWernery, R-
dc.contributor.authorWong, YM-
dc.contributor.authorJoseph, S-
dc.contributor.authorTsang, KL-
dc.contributor.authorPatteril, NA-
dc.contributor.authorElizabeth, SK-
dc.contributor.authorChan, KH-
dc.contributor.authorMuhammed, R-
dc.contributor.authorKinne, J-
dc.contributor.authorYuen, KY-
dc.contributor.authorWernery, U-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2017-09-18T02:05:19Z-
dc.date.available2017-09-18T02:05:19Z-
dc.date.issued2016-
dc.identifier.citationEmerging Microbes & Infections, 2016, v. 5, article no. e128-
dc.identifier.issn2222-1751-
dc.identifier.urihttp://hdl.handle.net/10722/245139-
dc.description.abstractLittle is known regarding the molecular epidemiology of Middle East respiratory syndrome coronavirus (MERS-CoV) circulating in dromedaries outside Saudi Arabia. To address this knowledge gap, we sequenced 10 complete genomes of MERS-CoVs isolated from 2 live and 8 dead dromedaries from different regions in the United Arab Emirates (UAE). Phylogenetic analysis revealed one novel clade A strain, the first detected in the UAE, and nine clade B strains. Strain D998/15 had a distinct phylogenetic position within clade A, being more closely related to the dromedary isolate NRCE-HKU205 from Egypt than to the human isolates EMC/2012 and Jordan-N3/2012. A comparison of predicted protein sequences also demonstrated the existence of two clade A lineages with unique amino acid substitutions, A1 (EMC/2012 and Jordan-N3/2012) and A2 (D998/15 and NRCE-HKU205), circulating in humans and camels, respectively. The nine clade B isolates belong to three distinct lineages: B1, B3 and B5. Two B3 strains, D1271/15 and D1189.1/15, showed evidence of recombination between lineages B4 and B5 in ORF1ab. Molecular clock analysis dated the time of the most recent common ancestor (tMRCA) of clade A to March 2011 and that of clade B to November 2011. Our data support a polyphyletic origin of MERS-CoV in dromedaries and the co-circulation of diverse MERS-CoVs including recombinant strains in the UAE.-
dc.languageeng-
dc.publisherNature Publishing Group for Shanghai Shangyixun Cultural Communication Company. The Journal's web site is located at http://www.nature.com/emi/marketing/index.html-
dc.relation.ispartofEmerging Microbes & Infections-
dc.titlePolyphyletic origin of MERS coronaviruses and isolation of a novel clade A strain from dromedary camels in the United Arab Emirates-
dc.typeArticle-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailWong, YM: emilyhk@hku.hk-
dc.identifier.emailChan, KH: chankh2@hkucc.hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityChan, KH=rp01921-
dc.identifier.authorityYuen, KY=rp00366-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/emi.2016.129-
dc.identifier.pmcidPMC5180373-
dc.identifier.hkuros275886-
dc.identifier.volume5-
dc.identifier.spagearticle no. e128-
dc.identifier.epagearticle no. e128-
dc.identifier.isiWOS:000391507900004-
dc.publisher.placeUnited Kingdom-

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