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Conference Paper: iPSC-derived sensory neurons for fate commitment of bone marrow-derived Schwann cells: Implication for re-myelination therapy

TitleiPSC-derived sensory neurons for fate commitment of bone marrow-derived Schwann cells: Implication for re-myelination therapy
Authors
Issue Date2016
PublisherFrontiers Research Foundation. The Abstracts' web site is located at https://www.frontiersin.org/events
Citation
14th Meeting of the Asian-Pacific Society for Neurochemistry (APSN 2016), Kuala Lumpur, Malaysia, 27-30 August 2016. In Frontiers in Cellular Neuroscience. Conference Abstract How to Cite?
AbstractThis is a sequel to our effort of deriving Schwann cells from bone marrow stromal cells (BMSCs) as a resource for translational research. Earlier studies succeeded as far as generating neurospheres from BMSCs of adult rats and demonstrating competence of the neurosphere-derived cells for directed differentiation to Schwann cell-like cells; these however reverted to a myofibroblast-like phenotype upon withdrawal of the differentiation-inducing factors. We took an alternative approach and selected five small-molecule inhibitors of key signaling pathways in an 8-day program to induce differentiation of human iPSCs into sensory neurons, reaching ≥80% yield in terms of marker proteins. Continuing culture in maintenance medium then found neuronal networks which were electrically excitable, showing tetrodotoxin-sensitive action potentials in patch-clamp experiments. Co-culturing the human iPSC-derived sensory neurons with BMSC-derived Schwann cell-like cells mediated juxtacrine signaling that effected the cell-intrinsic switch to the Schwann cell fate. Henceforth, the cell progeny maintained the Schwann cell phenotype without need for exogenous differentiation-inducing factors or neurons. These derived Schwann cells were functionally capable of myelination and neurotrophic support of neurite growth in in vitro assays, similar to sciatic nerve-derived Schwann cells.
DescriptionSymposium 11: Generating New Neurons: From Development to Cell-Based Therapy
Persistent Identifierhttp://hdl.handle.net/10722/244709

 

DC FieldValueLanguage
dc.contributor.authorShum, DKY-
dc.contributor.authorCai, S-
dc.contributor.authorAo, Q-
dc.contributor.authorHan, L-
dc.contributor.authorTsui, YP-
dc.contributor.authorChan, YS-
dc.date.accessioned2017-09-18T01:57:36Z-
dc.date.available2017-09-18T01:57:36Z-
dc.date.issued2016-
dc.identifier.citation14th Meeting of the Asian-Pacific Society for Neurochemistry (APSN 2016), Kuala Lumpur, Malaysia, 27-30 August 2016. In Frontiers in Cellular Neuroscience. Conference Abstract-
dc.identifier.urihttp://hdl.handle.net/10722/244709-
dc.descriptionSymposium 11: Generating New Neurons: From Development to Cell-Based Therapy-
dc.description.abstractThis is a sequel to our effort of deriving Schwann cells from bone marrow stromal cells (BMSCs) as a resource for translational research. Earlier studies succeeded as far as generating neurospheres from BMSCs of adult rats and demonstrating competence of the neurosphere-derived cells for directed differentiation to Schwann cell-like cells; these however reverted to a myofibroblast-like phenotype upon withdrawal of the differentiation-inducing factors. We took an alternative approach and selected five small-molecule inhibitors of key signaling pathways in an 8-day program to induce differentiation of human iPSCs into sensory neurons, reaching ≥80% yield in terms of marker proteins. Continuing culture in maintenance medium then found neuronal networks which were electrically excitable, showing tetrodotoxin-sensitive action potentials in patch-clamp experiments. Co-culturing the human iPSC-derived sensory neurons with BMSC-derived Schwann cell-like cells mediated juxtacrine signaling that effected the cell-intrinsic switch to the Schwann cell fate. Henceforth, the cell progeny maintained the Schwann cell phenotype without need for exogenous differentiation-inducing factors or neurons. These derived Schwann cells were functionally capable of myelination and neurotrophic support of neurite growth in in vitro assays, similar to sciatic nerve-derived Schwann cells.-
dc.languageeng-
dc.publisherFrontiers Research Foundation. The Abstracts' web site is located at https://www.frontiersin.org/events-
dc.relation.ispartofFrontiers in Cellular Neuroscience. Conference Abstract-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.titleiPSC-derived sensory neurons for fate commitment of bone marrow-derived Schwann cells: Implication for re-myelination therapy-
dc.typeConference_Paper-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.emailCai, S: caisa@hku.hk-
dc.identifier.emailHan, L: rahanlei@hku.hk-
dc.identifier.emailTsui, YP: alex2013@hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.authorityShum, DKY=rp00321-
dc.identifier.authorityChan, YS=rp00318-
dc.identifier.doi10.3389/conf.fncel.2016.36.00045-
dc.identifier.hkuros278970-
dc.publisher.placeSwitzerland-

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