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Article: Design, preparation, and selection of DNA-encoded dynamic libraries

TitleDesign, preparation, and selection of DNA-encoded dynamic libraries
Authors
Issue Date2015
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp
Citation
Chemical Science, 2015, v. 6 n. 12, p. 7097-7104 How to Cite?
AbstractWe report a method for the preparation and selection of DNA-encoded dynamic libraries (DEDLs). The library is composed of two sets of DNA-linked small molecules that are under dynamic exchange through DNA hybridization. Addition of the protein target shifted the equilibrium, favouring the assembly of high affinity bivalent binders. Notably, we introduced a novel locking mechanism to stop the dynamic exchange and “freeze” the equilibrium, thereby enabling downstream hit isolation and decoding by PCR amplification and DNA sequencing. Our DEDL approach has circumvented the limitation of library size and realized the analysis and selection of large dynamic libraries. In addition, this method also eliminates the requirement for modified and immobilized target proteins.
Persistent Identifierhttp://hdl.handle.net/10722/243010
ISSN
2017 Impact Factor: 9.063
2015 SCImago Journal Rankings: 4.974
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorLi, G-
dc.contributor.authorZheng, W-
dc.contributor.authorChen, Z-
dc.contributor.authorZhou, Y-
dc.contributor.authorLiu, Y-
dc.contributor.authorYang, J-
dc.contributor.authorHuang, Y-
dc.contributor.authorLi, X-
dc.date.accessioned2017-08-25T02:48:41Z-
dc.date.available2017-08-25T02:48:41Z-
dc.date.issued2015-
dc.identifier.citationChemical Science, 2015, v. 6 n. 12, p. 7097-7104-
dc.identifier.issn2041-6520-
dc.identifier.urihttp://hdl.handle.net/10722/243010-
dc.description.abstractWe report a method for the preparation and selection of DNA-encoded dynamic libraries (DEDLs). The library is composed of two sets of DNA-linked small molecules that are under dynamic exchange through DNA hybridization. Addition of the protein target shifted the equilibrium, favouring the assembly of high affinity bivalent binders. Notably, we introduced a novel locking mechanism to stop the dynamic exchange and “freeze” the equilibrium, thereby enabling downstream hit isolation and decoding by PCR amplification and DNA sequencing. Our DEDL approach has circumvented the limitation of library size and realized the analysis and selection of large dynamic libraries. In addition, this method also eliminates the requirement for modified and immobilized target proteins.-
dc.languageeng-
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp-
dc.relation.ispartofChemical Science-
dc.titleDesign, preparation, and selection of DNA-encoded dynamic libraries-
dc.typeArticle-
dc.identifier.emailZhou, Y: yuchow@hku.hk-
dc.identifier.emailLi, X: xiaoyuli@hku.hk-
dc.identifier.authorityLi, X=rp02080-
dc.identifier.doi10.1039/C5SC02467F-
dc.identifier.pmcidPMC5510007-
dc.identifier.hkuros274755-
dc.identifier.volume6-
dc.identifier.issue12-
dc.identifier.spage7097-
dc.identifier.epage7104-
dc.publisher.placeUnited Kingdom-

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