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Article: Absence of NUCKS augments paracrine effects of mesenchymal stem cells-mediated cardiac protection

TitleAbsence of NUCKS augments paracrine effects of mesenchymal stem cells-mediated cardiac protection
Authors
KeywordsNUCKS
Mesenchymal stem cells
NF-κB
Myocardial infarction
Issue Date2017
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcr
Citation
Experimental Cell Research, 2017, v. 356 n. 1, p. 74-84 How to Cite?
AbstractBone marrow-derived mesenchymal stem cells (BM-MSCs) contribute to myocardial repair after myocardial infarction (MI) by secreting a panel of growth factors and cytokines. This study was to investigate the potential mechanisms of the nuclear casein kinase and cyclin-dependent kinase substrate 1 (NUCKS) in regulation of the profiles of BM-MSCs secretion and compare the therapeutic efficacy of NUCKS-/-- and wide type-BM-MSCs (WT-BM-MSCs) on MI. The secretion profiles between NUCKS-/-- and WT-BM-MSCs under hypoxia (1%O2) were analyzed. Gene function analysis showed that compared with WT-BM-MSCs-conditioned medium (CdM), some genes over-presented in NUCKS-/--BM-MSCs-CdM were closely associated with inflammatory response, regulation of cell proliferation, death, migration and secretion. Notably, VEGFa in NUCKS-/--BM-MSCs-CdM was higher than that of WT-BM-MSCs-CdM. WT-BM-MSCs and NUCKS-/--BM-MSCs were transplanted into the peri-infarct region in mice of MI. At 4 weeks after cell transplantation, NUCKS-/-- or WT-BM-MSCs group significantly improved heart function and vessels density and reduced infarction size and apoptosis of cardiomyocytes. Furthermore, NUCKS-/--BM-MSCs provided better cardioprotective effects than WT-BM-MSCs against MI. Our study demonstrates that depletion of NUCKS enhances the therapeutic efficacy of BM-MSCs for MI via regulating the secretion.
Persistent Identifierhttp://hdl.handle.net/10722/241316
ISSN
2017 Impact Factor: 3.309
2015 SCImago Journal Rankings: 1.900
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, Y-
dc.contributor.authorChiu, S-
dc.contributor.authorLiang, X-
dc.contributor.authorChai, YH-
dc.contributor.authorQin, Y-
dc.contributor.authorWang, JJ-
dc.contributor.authorLi, X-
dc.contributor.authorQiu, B-
dc.contributor.authorTergaonkar, V-
dc.contributor.authorTse, HF-
dc.contributor.authorLian, Q-
dc.date.accessioned2017-06-05T08:24:42Z-
dc.date.available2017-06-05T08:24:42Z-
dc.date.issued2017-
dc.identifier.citationExperimental Cell Research, 2017, v. 356 n. 1, p. 74-84-
dc.identifier.issn0014-4827-
dc.identifier.urihttp://hdl.handle.net/10722/241316-
dc.description.abstractBone marrow-derived mesenchymal stem cells (BM-MSCs) contribute to myocardial repair after myocardial infarction (MI) by secreting a panel of growth factors and cytokines. This study was to investigate the potential mechanisms of the nuclear casein kinase and cyclin-dependent kinase substrate 1 (NUCKS) in regulation of the profiles of BM-MSCs secretion and compare the therapeutic efficacy of NUCKS-/-- and wide type-BM-MSCs (WT-BM-MSCs) on MI. The secretion profiles between NUCKS-/-- and WT-BM-MSCs under hypoxia (1%O2) were analyzed. Gene function analysis showed that compared with WT-BM-MSCs-conditioned medium (CdM), some genes over-presented in NUCKS-/--BM-MSCs-CdM were closely associated with inflammatory response, regulation of cell proliferation, death, migration and secretion. Notably, VEGFa in NUCKS-/--BM-MSCs-CdM was higher than that of WT-BM-MSCs-CdM. WT-BM-MSCs and NUCKS-/--BM-MSCs were transplanted into the peri-infarct region in mice of MI. At 4 weeks after cell transplantation, NUCKS-/-- or WT-BM-MSCs group significantly improved heart function and vessels density and reduced infarction size and apoptosis of cardiomyocytes. Furthermore, NUCKS-/--BM-MSCs provided better cardioprotective effects than WT-BM-MSCs against MI. Our study demonstrates that depletion of NUCKS enhances the therapeutic efficacy of BM-MSCs for MI via regulating the secretion.-
dc.languageeng-
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexcr-
dc.relation.ispartofExperimental Cell Research-
dc.rightsPosting accepted manuscript (postprint): © <year>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.subjectNUCKS-
dc.subjectMesenchymal stem cells-
dc.subjectNF-κB-
dc.subjectMyocardial infarction-
dc.titleAbsence of NUCKS augments paracrine effects of mesenchymal stem cells-mediated cardiac protection-
dc.typeArticle-
dc.identifier.emailZhang, Y: zhangyuelin1999@163.com-
dc.identifier.emailWang, JJ: junwen@hku.hk-
dc.identifier.emailTse, HF: hftse@hkucc.hku.hk-
dc.identifier.emailLian, Q: qzlian@hkucc.hku.hk-
dc.identifier.authorityWang, JJ=rp00280-
dc.identifier.authorityTse, HF=rp00428-
dc.identifier.authorityLian, Q=rp00267-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.yexcr.2017.04.012-
dc.identifier.pmid28412246-
dc.identifier.scopuseid_2-s2.0-85017473274-
dc.identifier.hkuros273903-
dc.identifier.hkuros276956-
dc.identifier.volume356-
dc.identifier.issue1-
dc.identifier.spage74-
dc.identifier.epage84-
dc.identifier.isiWOS:000402776700009-
dc.publisher.placeUnited States-

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