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Article: Inhibition of NUCKS Facilitates Corneal Recovery Following Alkali Burn

TitleInhibition of NUCKS Facilitates Corneal Recovery Following Alkali Burn
Authors
Issue Date2017
PublisherNature Publishing Group: Open Access Journals. The Journal's web site is located at http://www.nature.com/srep/index.html
Citation
Scientific Reports, 2017, v. 7, p. Article no. 41224 How to Cite?
AbstractCorneal wound healing involves a complex cascade of cytokine-controlled cellular events, including inflammatory and angiogenesis responses that are regulated by transcriptional chromatin remodeling. Nuclear Ubiquitous Casein and cyclin-dependent Kinase Substrate (NUCKS) is a key chromatin modifier and transcriptional regulator of metabolic signaling. In this study, we investigated the role of NUCKS in corneal wound healing by comparing its effects on corneal alkali burn in NUCKS knockout (NKO) and NUCKS wild-type (NWT) mice. Our data showed that following alkali-injury, inhibition of NUCKS (NKO) accelerated ocular resurfacing and suppressed neovascularization; the cytokine profile of alkali burned corneas in NKO mice showed suppressed expression of inflammation cytokines (IL1A &IL1B); upregulated expression of antiangiogenic factor (Pigment Epithelium-derived Factor; PEDF); and downregulated expression of angiogenic factor (Vascular Endothelial Growth Factor, VEGF); in vitro, following LPS-induced NFκB activation, NKO corneal cells showed reduced expression of IL6, IP10 and TNFα. In vitro, corneal epithelial cells showed reduced NF-κb activation on silencing of NUCKS and corresponding NFκB-mediated cytokine expression was reduced. Here, we illustrate that inhibition of NUCKS played a role in cytokine modulation and facilitated corneal recovery. This reveals a potential new effective strategy for ocular burn treatment.
Persistent Identifierhttp://hdl.handle.net/10722/241315
ISSN
2017 Impact Factor: 4.122
2015 SCImago Journal Rankings: 2.073
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPoon, MW-
dc.contributor.authorJiang, D-
dc.contributor.authorQin, P-
dc.contributor.authorZhang, Y-
dc.contributor.authorQiu, B-
dc.contributor.authorChanda, S-
dc.contributor.authorTergaonkar, V-
dc.contributor.authorLi, Q-
dc.contributor.authorWong, YHI-
dc.contributor.authorYu, Z-
dc.contributor.authorTse, HF-
dc.contributor.authorWong, DSH-
dc.contributor.authorLian, Q-
dc.date.accessioned2017-06-05T08:13:07Z-
dc.date.available2017-06-05T08:13:07Z-
dc.date.issued2017-
dc.identifier.citationScientific Reports, 2017, v. 7, p. Article no. 41224-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10722/241315-
dc.description.abstractCorneal wound healing involves a complex cascade of cytokine-controlled cellular events, including inflammatory and angiogenesis responses that are regulated by transcriptional chromatin remodeling. Nuclear Ubiquitous Casein and cyclin-dependent Kinase Substrate (NUCKS) is a key chromatin modifier and transcriptional regulator of metabolic signaling. In this study, we investigated the role of NUCKS in corneal wound healing by comparing its effects on corneal alkali burn in NUCKS knockout (NKO) and NUCKS wild-type (NWT) mice. Our data showed that following alkali-injury, inhibition of NUCKS (NKO) accelerated ocular resurfacing and suppressed neovascularization; the cytokine profile of alkali burned corneas in NKO mice showed suppressed expression of inflammation cytokines (IL1A &IL1B); upregulated expression of antiangiogenic factor (Pigment Epithelium-derived Factor; PEDF); and downregulated expression of angiogenic factor (Vascular Endothelial Growth Factor, VEGF); in vitro, following LPS-induced NFκB activation, NKO corneal cells showed reduced expression of IL6, IP10 and TNFα. In vitro, corneal epithelial cells showed reduced NF-κb activation on silencing of NUCKS and corresponding NFκB-mediated cytokine expression was reduced. Here, we illustrate that inhibition of NUCKS played a role in cytokine modulation and facilitated corneal recovery. This reveals a potential new effective strategy for ocular burn treatment.-
dc.languageeng-
dc.publisherNature Publishing Group: Open Access Journals. The Journal's web site is located at http://www.nature.com/srep/index.html-
dc.relation.ispartofScientific Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleInhibition of NUCKS Facilitates Corneal Recovery Following Alkali Burn-
dc.typeArticle-
dc.identifier.emailZhang, Y: zhangyuelin1999@163.com-
dc.identifier.emailLi, Q: qinglee@hku.hk-
dc.identifier.emailWong, YHI: wongyhi@hku.hk-
dc.identifier.emailWong, DSH: shdwong@hku.hk-
dc.identifier.emailLian, Q: qzlian@hkucc.hku.hk-
dc.identifier.authorityLi, Q=rp01741-
dc.identifier.authorityWong, YHI=rp01467-
dc.identifier.authorityWong, DSH=rp00516-
dc.identifier.authorityLian, Q=rp00267-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/srep41224-
dc.identifier.pmid28106169-
dc.identifier.pmcidPMC5247723-
dc.identifier.scopuseid_2-s2.0-85009992320-
dc.identifier.hkuros274465-
dc.identifier.hkuros280695-
dc.identifier.volume7-
dc.identifier.spageArticle no. 41224-
dc.identifier.epageArticle no. 41224-
dc.identifier.isiWOS:000392328500001-
dc.publisher.placeUnited Kingdom-

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