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Article: Deep sequencing analysis of quasispecies in the HBV pre-S region and its association with hepatocellular carcinoma

TitleDeep sequencing analysis of quasispecies in the HBV pre-S region and its association with hepatocellular carcinoma
Authors
KeywordsDeep sequencing
Hepatitis B virus
Hepatocellular carcinoma
Pre-S deletions
Quasispecies
Issue Date2017
PublisherSpringer Japan. The Journal's web site is located at http://link.springer-ny.com/link/service/journals/00535/index.htm
Citation
Journal of Gastroenterology, 2017, v. 52 n. 9, p. 1064-1074 How to Cite?
AbstractBACKGROUND: The association between the evolution of hepatitis B virus (HBV) quasispecies and the development of hepatocellular carcinoma (HCC) is unknown. METHODS: We used deep sequencing to examine the dynamics of HBV quasispecies and their relationship to HCC development. Thirty-two chronic hepatitis B (CHB) patients with HCC (HCC group) and 32 matched CHB patients without HCC (controls) were recruited. Fourteen patients from each group had serial sera available up to 9 years before the time of the present study. Deep sequencing of the HBV pre-S regions was performed. HBV quasispecies complexity, diversity, and intrapatient prevalence of pre-S deletions/mutations were analyzed. RESULTS: Compared with control patients, HCC patients had a significant greater quasispecies complexity (p = 0.04 at the nucleotide level), greater diversity (p = 0.004 and 0.009 at the nucleotide level and the amino acid level respectively), and a trend of greater complexity at the amino acid level (p = 0.065). HCC patients had a higher intrapatient prevalence of pre-S deletions and point mutations (at codons 4, 27, and 167) compared with the control patients (all p < 0.05). Longitudinal observation in the sera of 14 HCC patients showed that quasispecies complexity (p = 0.027 and 0.024 at the nucleotide level and the amino acid level respectively) and diversity (p = 0.035 and 0.031 at the nucleotide level and the amino acid level respectively) increased as the disease progressed to HCC. CONCLUSIONS: Increased HBV quasispecies complexity and diversity in the pre-S region, probably reflecting enhanced virus-host interplay, was associated with disease progression from CHB to HCC.
Persistent Identifierhttp://hdl.handle.net/10722/240254
ISSN
2017 Impact Factor: 5.561
2015 SCImago Journal Rankings: 1.651
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, AY-
dc.contributor.authorLai, CL-
dc.contributor.authorHuang, FY-
dc.contributor.authorSeto, WKW-
dc.contributor.authorFung, JYY-
dc.contributor.authorWong, DKH-
dc.contributor.authorYuen, RMF-
dc.date.accessioned2017-04-19T08:21:57Z-
dc.date.available2017-04-19T08:21:57Z-
dc.date.issued2017-
dc.identifier.citationJournal of Gastroenterology, 2017, v. 52 n. 9, p. 1064-1074-
dc.identifier.issn0944-1174-
dc.identifier.urihttp://hdl.handle.net/10722/240254-
dc.description.abstractBACKGROUND: The association between the evolution of hepatitis B virus (HBV) quasispecies and the development of hepatocellular carcinoma (HCC) is unknown. METHODS: We used deep sequencing to examine the dynamics of HBV quasispecies and their relationship to HCC development. Thirty-two chronic hepatitis B (CHB) patients with HCC (HCC group) and 32 matched CHB patients without HCC (controls) were recruited. Fourteen patients from each group had serial sera available up to 9 years before the time of the present study. Deep sequencing of the HBV pre-S regions was performed. HBV quasispecies complexity, diversity, and intrapatient prevalence of pre-S deletions/mutations were analyzed. RESULTS: Compared with control patients, HCC patients had a significant greater quasispecies complexity (p = 0.04 at the nucleotide level), greater diversity (p = 0.004 and 0.009 at the nucleotide level and the amino acid level respectively), and a trend of greater complexity at the amino acid level (p = 0.065). HCC patients had a higher intrapatient prevalence of pre-S deletions and point mutations (at codons 4, 27, and 167) compared with the control patients (all p < 0.05). Longitudinal observation in the sera of 14 HCC patients showed that quasispecies complexity (p = 0.027 and 0.024 at the nucleotide level and the amino acid level respectively) and diversity (p = 0.035 and 0.031 at the nucleotide level and the amino acid level respectively) increased as the disease progressed to HCC. CONCLUSIONS: Increased HBV quasispecies complexity and diversity in the pre-S region, probably reflecting enhanced virus-host interplay, was associated with disease progression from CHB to HCC.-
dc.languageeng-
dc.publisherSpringer Japan. The Journal's web site is located at http://link.springer-ny.com/link/service/journals/00535/index.htm-
dc.relation.ispartofJournal of Gastroenterology-
dc.subjectDeep sequencing-
dc.subjectHepatitis B virus-
dc.subjectHepatocellular carcinoma-
dc.subjectPre-S deletions-
dc.subjectQuasispecies-
dc.titleDeep sequencing analysis of quasispecies in the HBV pre-S region and its association with hepatocellular carcinoma-
dc.typeArticle-
dc.identifier.emailLai, CL: hrmelcl@hku.hk-
dc.identifier.emailHuang, FY: camy@graduate.hku.hk-
dc.identifier.emailSeto, WKW: wkseto2@hku.hk-
dc.identifier.emailFung, JYY: jfung@hkucc.hku.hk-
dc.identifier.emailWong, DKH: danywong@hku.hk-
dc.identifier.emailYuen, RMF: mfyuen@hku.hk-
dc.identifier.authorityLai, CL=rp00314-
dc.identifier.authoritySeto, WKW=rp01659-
dc.identifier.authorityFung, JYY=rp00518-
dc.identifier.authorityWong, DKH=rp00492-
dc.identifier.authorityYuen, RMF=rp00479-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00535-017-1334-1-
dc.identifier.pmid28353014-
dc.identifier.scopuseid_2-s2.0-85016113608-
dc.identifier.hkuros271887-
dc.identifier.volume52-
dc.identifier.issue9-
dc.identifier.spage1064-
dc.identifier.epage1074-
dc.identifier.isiWOS:000408230400007-
dc.publisher.placeJapan-

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