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Article: Mutagenesis and genome engineering of Epstein-Barr virus in cultured human cells by CRISPR/Cas9

TitleMutagenesis and genome engineering of Epstein-Barr virus in cultured human cells by CRISPR/Cas9
Authors
Issue Date2016
PublisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651
Citation
Methods in Molecular Biology, 2016, v. 1498, p. 23-31 How to Cite?
AbstractThe clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 nuclease (Cas9) system is a powerful genome-editing tool for both chromosomal and extrachromosomal DNA. DNA viruses such as Epstein–Barr virus (EBV), which undergoes episomal replication in human cells, can be effectively edited by CRISPR/Cas9. We have demonstrated targeted editing of the EBV genome by CRISPR/Cas9 in several lines of EBV-infected cells. CRISPR/Cas9-based mutagenesis and genome engineering of EBV provides a new method for genetic analysis, which has some advantages over bacterial artificial chromosome-based recombineering. This approach might also prove useful in the cure of EBV infection. In this chapter, we use the knockout of the BART promoter as an example to detail the experimental procedures for construction of recombinant EBV in human cells.
Persistent Identifierhttp://hdl.handle.net/10722/238618
ISBN
ISSN
2015 SCImago Journal Rankings: 0.549

 

DC FieldValueLanguage
dc.contributor.authorYuen, KS-
dc.contributor.authorChan, CP-
dc.contributor.authorKok, KH-
dc.contributor.authorJin, D-
dc.date.accessioned2017-02-20T01:23:52Z-
dc.date.available2017-02-20T01:23:52Z-
dc.date.issued2016-
dc.identifier.citationMethods in Molecular Biology, 2016, v. 1498, p. 23-31-
dc.identifier.isbn9781493964727-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/238618-
dc.description.abstractThe clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 nuclease (Cas9) system is a powerful genome-editing tool for both chromosomal and extrachromosomal DNA. DNA viruses such as Epstein–Barr virus (EBV), which undergoes episomal replication in human cells, can be effectively edited by CRISPR/Cas9. We have demonstrated targeted editing of the EBV genome by CRISPR/Cas9 in several lines of EBV-infected cells. CRISPR/Cas9-based mutagenesis and genome engineering of EBV provides a new method for genetic analysis, which has some advantages over bacterial artificial chromosome-based recombineering. This approach might also prove useful in the cure of EBV infection. In this chapter, we use the knockout of the BART promoter as an example to detail the experimental procedures for construction of recombinant EBV in human cells.-
dc.languageeng-
dc.publisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651-
dc.relation.ispartofMethods in Molecular Biology-
dc.rightsThe final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-6472-7_2-
dc.titleMutagenesis and genome engineering of Epstein-Barr virus in cultured human cells by CRISPR/Cas9-
dc.typeArticle-
dc.identifier.emailYuen, KS: samyuen@hku.hk-
dc.identifier.emailChan, CP: chancp10@hku.hk-
dc.identifier.emailKok, KH: khkok@hku.hk-
dc.identifier.emailJin, D: dyjin@hku.hk-
dc.identifier.authorityChan, CP=rp02031-
dc.identifier.authorityKok, KH=rp01455-
dc.identifier.authorityJin, D=rp00452-
dc.description.naturepostprint-
dc.identifier.doi10.1007/978-1-4939-6472-7_2-
dc.identifier.hkuros271259-
dc.identifier.volume1498-
dc.identifier.spage23-
dc.identifier.epage31-
dc.publisher.placeUnited States-

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