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postgraduate thesis: Development and evaluation of a novel in-house single-tube nested real-time polymerase chain reaction assay with FilmArray respiratory panel for detection of adenovirus

TitleDevelopment and evaluation of a novel in-house single-tube nested real-time polymerase chain reaction assay with FilmArray respiratory panel for detection of adenovirus
Authors
Issue Date2016
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
Citation
Law, K. [羅健明]. (2016). Development and evaluation of a novel in-house single-tube nested real-time polymerase chain reaction assay with FilmArray respiratory panel for detection of adenovirus. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR.
AbstractFilmArray is a rapid diagnostic technology which can simultaneously detect multiple pathogens in a single run. It is especially useful in the diagnosis of respiratory diseases because most of the causative pathogens cause similar symptoms. While FilmArray has numerous undisputable advantages in the detection of respiratory pathogens, one of the major concerns is that it has poor sensitivity for adenovirus detection. Reports are consistently showing that the FilmArray Respiratory Panel has only around 50% to 60% sensitivity for the detection of adenoviruses. FilmArray Respiratory Panel’s poor sensitivity for adenovirus detection is of particular concern for immunocompromised patients because they are at higher risk of developing severe infection. Therefore, a complementary test would be needed to overcome these limitations of FilmArray. In this study, a novel in-house single-tube nested real-time polymerase chain reaction assay was developed and we evaluated its performance with that of FilmArray Respiratory Panel for adenovirus detection. Improved performance for detection of adenoviruses has been shown for the in-house single-tube nested real-time polymerase chain reaction assay. Eight additional samples that were tested negative by FilmArray Respiratory Panel were tested positive by the single-tube nested real-time polymerase chain reaction assay, with low viral loads of 3.2 to 1.8 x 〖10〗^4 DNA copies/reaction. The use of this single-tube nested real-time polymerase chain reaction assay should be considered in patients with clinical suspicion of adenovirus but a negative FilmArray Respiratory Panel result.
DegreeMaster of Medical Sciences
SubjectAdenoviruses
Dept/ProgramMicrobiology
Persistent Identifierhttp://hdl.handle.net/10722/237229
HKU Library Item IDb5804743

 

DC FieldValueLanguage
dc.contributor.authorLaw, Kin-ming-
dc.contributor.author羅健明-
dc.date.accessioned2016-12-28T02:01:54Z-
dc.date.available2016-12-28T02:01:54Z-
dc.date.issued2016-
dc.identifier.citationLaw, K. [羅健明]. (2016). Development and evaluation of a novel in-house single-tube nested real-time polymerase chain reaction assay with FilmArray respiratory panel for detection of adenovirus. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR.-
dc.identifier.urihttp://hdl.handle.net/10722/237229-
dc.description.abstractFilmArray is a rapid diagnostic technology which can simultaneously detect multiple pathogens in a single run. It is especially useful in the diagnosis of respiratory diseases because most of the causative pathogens cause similar symptoms. While FilmArray has numerous undisputable advantages in the detection of respiratory pathogens, one of the major concerns is that it has poor sensitivity for adenovirus detection. Reports are consistently showing that the FilmArray Respiratory Panel has only around 50% to 60% sensitivity for the detection of adenoviruses. FilmArray Respiratory Panel’s poor sensitivity for adenovirus detection is of particular concern for immunocompromised patients because they are at higher risk of developing severe infection. Therefore, a complementary test would be needed to overcome these limitations of FilmArray. In this study, a novel in-house single-tube nested real-time polymerase chain reaction assay was developed and we evaluated its performance with that of FilmArray Respiratory Panel for adenovirus detection. Improved performance for detection of adenoviruses has been shown for the in-house single-tube nested real-time polymerase chain reaction assay. Eight additional samples that were tested negative by FilmArray Respiratory Panel were tested positive by the single-tube nested real-time polymerase chain reaction assay, with low viral loads of 3.2 to 1.8 x 〖10〗^4 DNA copies/reaction. The use of this single-tube nested real-time polymerase chain reaction assay should be considered in patients with clinical suspicion of adenovirus but a negative FilmArray Respiratory Panel result.-
dc.languageeng-
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)-
dc.relation.ispartofHKU Theses Online (HKUTO)-
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subject.lcshAdenoviruses-
dc.titleDevelopment and evaluation of a novel in-house single-tube nested real-time polymerase chain reaction assay with FilmArray respiratory panel for detection of adenovirus-
dc.typePG_Thesis-
dc.identifier.hkulb5804743-
dc.description.thesisnameMaster of Medical Sciences-
dc.description.thesislevelMaster-
dc.description.thesisdisciplineMicrobiology-
dc.description.naturepublished_or_final_version-

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