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Article: RbAp46/48LIN-53 is Required for Holocentromere Assembly in Caenorhabditis elegans

TitleRbAp46/48<font size=-1><sup>LIN-53</sup></font> is Required for Holocentromere Assembly in Caenorhabditis elegans
Authors
LEE, CHLIN, ZYuen, KWY
Issue Date2016
PublisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports
Citation
Cell Reports, 2016, v. 14 n. 8, p. 1819-1828 How to Cite?
DOI: http://dx.doi.org/10.1016/j.celrep.2016.01.065
AbstractCentromeres, the specialized chromosomal regions for recruiting kinetochores and directing chromosome segregation, are epigenetically marked by a centromeric histone H3 variant, CENP-A. To maintain centromere identity through cell cycles, CENP-A diluted during DNA replication is replenished. The licensing factor M18BP1KNL-2 is known to recruit CENP-A to holocentromeres. Here, we show that RbAp46/48LIN-53, a conserved histone chaperone, is required for CENP-AHCP-3 localization in holocentric Caenorhabditis elegans. Indeed, RbAp46/48LIN-53 and CENP-AHCP-3 localizations are interdependent. RbAp46/48LIN-53 localizes to the centromere during metaphase in a CENP-AHCP-3- and M18BP1KNL-2-dependent manner, suggesting CENP-AHCP-3 loading may occur before anaphase. RbAp46/48LIN-53 does not function at the centromere through histone acetylation, H3K27 trimethylation, or its known chromatin-modifying complexes. RbAp46/48LIN-53 may function independently to escort CENP-AHCP-3 for holocentromere assembly but is dispensable for other kinetochore protein recruitment. Nonetheless, depletion of RbAp46/48LIN-53 leads to anaphase bridges and chromosome missegregation. This study unravels the holocentromere assembly hierarchy and its conservation with monocentromeres.
Persistent Identifierhttp://hdl.handle.net/10722/235354
ISSN
2211-1247
2021 Impact Factor: 9.995
2020 SCImago Journal Rankings: 6.264
ISI Accession Number ID
WOS:000371217700002

 

DC FieldValueLanguage
dc.contributor.authorLEE, CH-
dc.contributor.authorLIN, Z-
dc.contributor.authorYuen, KWY-
dc.date.accessioned2016-10-14T13:52:45Z-
dc.date.available2016-10-14T13:52:45Z-
dc.date.issued2016-
dc.identifier.citationCell Reports, 2016, v. 14 n. 8, p. 1819-1828-
dc.identifier.issn2211-1247-
dc.identifier.urihttp://hdl.handle.net/10722/235354-
dc.description.abstractCentromeres, the specialized chromosomal regions for recruiting kinetochores and directing chromosome segregation, are epigenetically marked by a centromeric histone H3 variant, CENP-A. To maintain centromere identity through cell cycles, CENP-A diluted during DNA replication is replenished. The licensing factor M18BP1KNL-2 is known to recruit CENP-A to holocentromeres. Here, we show that RbAp46/48LIN-53, a conserved histone chaperone, is required for CENP-AHCP-3 localization in holocentric Caenorhabditis elegans. Indeed, RbAp46/48LIN-53 and CENP-AHCP-3 localizations are interdependent. RbAp46/48LIN-53 localizes to the centromere during metaphase in a CENP-AHCP-3- and M18BP1KNL-2-dependent manner, suggesting CENP-AHCP-3 loading may occur before anaphase. RbAp46/48LIN-53 does not function at the centromere through histone acetylation, H3K27 trimethylation, or its known chromatin-modifying complexes. RbAp46/48LIN-53 may function independently to escort CENP-AHCP-3 for holocentromere assembly but is dispensable for other kinetochore protein recruitment. Nonetheless, depletion of RbAp46/48LIN-53 leads to anaphase bridges and chromosome missegregation. This study unravels the holocentromere assembly hierarchy and its conservation with monocentromeres.-
dc.languageeng-
dc.publisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports-
dc.relation.ispartofCell Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleRbAp46/48<font size=-1><sup>LIN-53</sup></font> is Required for Holocentromere Assembly in Caenorhabditis elegans-
dc.typeArticle-
dc.identifier.emailYuen, KWY: kwyyuen@hku.hk-
dc.identifier.authorityYuen, KWY=rp01512-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.celrep.2016.01.065-
dc.identifier.scopuseid_2-s2.0-84959238490-
dc.identifier.hkuros268840-
dc.identifier.volume14-
dc.identifier.issue8-
dc.identifier.spage1819-
dc.identifier.epage1828-
dc.identifier.isiWOS:000371217700002-
dc.publisher.placeUnited States-
dc.identifier.issnl2211-1247-

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