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Conference Paper: Kinetics of enzymatic hydrolysis revealed by video rate AFM single molecule analysis

TitleKinetics of enzymatic hydrolysis revealed by video rate AFM single molecule analysis
Authors
Issue Date2015
PublisherIEEE.
Citation
The 15th IEEE International Conference on Nanotechnology (IEEE-NANO 2015), Rome, Italy, 27-30 July 2015. In Conference Proceedings, 2015, p. 1288-1291 How to Cite?
AbstractThe rapid growing bio-refining industry is hindered by the slow reaction rate of cellobiohydrolase catalyzed hydrolysis. Analysis of the mechanism of enzyme interaction in the cellobiohydrolase catalyzed hydrolysis is difficult because of the variety factors affecting the enzymatic reaction rate. In this study, we evaluated the processive hydrolysis parameters in the molecular level using a video rate atomic force microscopy (AFM). Both dissociate and association rates (koff and kon) have been calculated by an experimental data fitted Michaelis-Menten model and real-time observation experimental data. The difference in these two association rates suggested that the cellulose substrate governs the hydrolysis rate and that can deepen the understanding of the mechanisms of processive hydrolysis. © 2015 IEEE.
Persistent Identifierhttp://hdl.handle.net/10722/235005
ISBN

 

DC FieldValueLanguage
dc.contributor.authorSong, B-
dc.contributor.authorXi, N-
dc.contributor.authorSun, Z-
dc.contributor.authorYang, R-
dc.contributor.authorChen, L-
dc.contributor.authorZhou, Z-
dc.contributor.authorYang, Y-
dc.contributor.authorDu, W-
dc.contributor.authorXi, J-
dc.date.accessioned2016-10-14T13:50:40Z-
dc.date.available2016-10-14T13:50:40Z-
dc.date.issued2015-
dc.identifier.citationThe 15th IEEE International Conference on Nanotechnology (IEEE-NANO 2015), Rome, Italy, 27-30 July 2015. In Conference Proceedings, 2015, p. 1288-1291-
dc.identifier.isbn978-146738155-0-
dc.identifier.urihttp://hdl.handle.net/10722/235005-
dc.description.abstractThe rapid growing bio-refining industry is hindered by the slow reaction rate of cellobiohydrolase catalyzed hydrolysis. Analysis of the mechanism of enzyme interaction in the cellobiohydrolase catalyzed hydrolysis is difficult because of the variety factors affecting the enzymatic reaction rate. In this study, we evaluated the processive hydrolysis parameters in the molecular level using a video rate atomic force microscopy (AFM). Both dissociate and association rates (koff and kon) have been calculated by an experimental data fitted Michaelis-Menten model and real-time observation experimental data. The difference in these two association rates suggested that the cellulose substrate governs the hydrolysis rate and that can deepen the understanding of the mechanisms of processive hydrolysis. © 2015 IEEE.-
dc.languageeng-
dc.publisherIEEE.-
dc.relation.ispartofIEEE International Conference on Nanotechnology (IEEE-NANO) Proceedings-
dc.rightsIEEE International Conference on Nanotechnology (IEEE-NANO) Proceedings. Copyright © IEEE.-
dc.rights©2015 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.-
dc.titleKinetics of enzymatic hydrolysis revealed by video rate AFM single molecule analysis-
dc.typeConference_Paper-
dc.identifier.emailXi, N: xining@hku.hk-
dc.identifier.authorityXi, N=rp02044-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1109/NANO.2015.7388868-
dc.identifier.scopuseid_2-s2.0-84964355798-
dc.identifier.hkuros269335-
dc.identifier.spage1288-
dc.identifier.epage1291-
dc.publisher.placeUnited States-
dc.customcontrol.immutablesml 161018-

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