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Conference Paper: Schwann cells demonstrated lineage plasticity in culture and acquired oligodendrocyte phenotypes

TitleSchwann cells demonstrated lineage plasticity in culture and acquired oligodendrocyte phenotypes
Authors
Issue Date2016
Citation
The 2016 Gordon Research Seminar (GRS) on Molecular & Cellular Neurobiology, The Hong Kong University of Science and Technology Hong Kong, China, 11-12 June 2016. How to Cite?
AbstractSchwann cells and oligodendrocytes are myelin-forming glia found in the peripheral and central nervous systems, respectively. Despite being similar in function, the two cell types have distinct developmental origins. In cultured Schwann cells, however, we serendipitously observed expression of the oligodendrocyte lineage fate determining factor Olig2. Purified Schwann cell cultures were prepared from neonatal rat sciatic nerves. Olig2-positive Schwann cells (OL2-SCs) were detected at 25-30 DIV. By 50-60 DIV, OL2-SCs acquired polydendritic morphology typical of oligodendrocyte precursors. This was accompanied by a decline in Schwann cell marker expression. The OP-like cells were termed Schwann cell-derived oligodendrocyte precursors (SC-OP). We therefore hypothesized that the peripheral nervous system environment is essential for the maintenance of Schwann cell identity. Co-culture of OL2-SCs with dorsal root ganglia neurons prevented conversion in SC-OPs. In contrast, SC-OPs co-cultured with dorsal root ganglia neurons continued differentiation into mature oligodendrocyte-like cells with myelin basic protein-positive segments along multiple axons. Our results revealed 'lineage switching' capability of Schwann cells isolated from the peripheral nervous system. Preservation of Schwann cell identity in vitro requires signalling cues derived from peripheral neurons.
DescriptionConference Theme: New and Emerging Technologies in Studying Neural Development, Plasticity, and Diseases
Session - Neural Lineage Specification and Plasticity
Persistent Identifierhttp://hdl.handle.net/10722/234411

 

DC FieldValueLanguage
dc.contributor.authorTsui, YP-
dc.contributor.authorTam, KW-
dc.contributor.authorShum, DKY-
dc.contributor.authorChan, YS-
dc.date.accessioned2016-10-14T13:46:42Z-
dc.date.available2016-10-14T13:46:42Z-
dc.date.issued2016-
dc.identifier.citationThe 2016 Gordon Research Seminar (GRS) on Molecular & Cellular Neurobiology, The Hong Kong University of Science and Technology Hong Kong, China, 11-12 June 2016.-
dc.identifier.urihttp://hdl.handle.net/10722/234411-
dc.descriptionConference Theme: New and Emerging Technologies in Studying Neural Development, Plasticity, and Diseases-
dc.descriptionSession - Neural Lineage Specification and Plasticity-
dc.description.abstractSchwann cells and oligodendrocytes are myelin-forming glia found in the peripheral and central nervous systems, respectively. Despite being similar in function, the two cell types have distinct developmental origins. In cultured Schwann cells, however, we serendipitously observed expression of the oligodendrocyte lineage fate determining factor Olig2. Purified Schwann cell cultures were prepared from neonatal rat sciatic nerves. Olig2-positive Schwann cells (OL2-SCs) were detected at 25-30 DIV. By 50-60 DIV, OL2-SCs acquired polydendritic morphology typical of oligodendrocyte precursors. This was accompanied by a decline in Schwann cell marker expression. The OP-like cells were termed Schwann cell-derived oligodendrocyte precursors (SC-OP). We therefore hypothesized that the peripheral nervous system environment is essential for the maintenance of Schwann cell identity. Co-culture of OL2-SCs with dorsal root ganglia neurons prevented conversion in SC-OPs. In contrast, SC-OPs co-cultured with dorsal root ganglia neurons continued differentiation into mature oligodendrocyte-like cells with myelin basic protein-positive segments along multiple axons. Our results revealed 'lineage switching' capability of Schwann cells isolated from the peripheral nervous system. Preservation of Schwann cell identity in vitro requires signalling cues derived from peripheral neurons.-
dc.languageeng-
dc.relation.ispartofMolecular & Cellular Neurobiology GRS-
dc.titleSchwann cells demonstrated lineage plasticity in culture and acquired oligodendrocyte phenotypes-
dc.typeConference_Paper-
dc.identifier.emailTsui, YP: alex2013@hku.hk-
dc.identifier.emailTam, KW: tamkw@hku.hk-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.authorityShum, DKY=rp00321-
dc.identifier.authorityChan, YS=rp00318-
dc.identifier.hkuros268596-

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