File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: Hepatitis B virus core-related antigen as a surrogate marker for covalently closed circular DNA

TitleHepatitis B virus core-related antigen as a surrogate marker for covalently closed circular DNA
Authors
Issue Date2016
PublisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0
Citation
The 25th Annual Conference of Asian Pacific Association for the Study of the Liver (APASL 2016), Tokyo, Japan, 20-24 February 2016. In Hepatology International, 2016, v. 10 n. 1 suppl., p. S42, abstract no. O-097 How to Cite?
AbstractBACKGROUND: Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) is a key to viral persistence in chronic hepatitis B patients. We aimed to explore whether serum hepatitis B core-related antigen (HBcrAg) could be a surrogate marker for intrahepatic cccDNA in a large cohort of patients with liver biopsies. METHODS: 305 liver biopsies and the corresponding sera collected from 138 nucleoside/tide analogues-treated patients were analyzed. 124 patients had paired liver biopsies at baseline and 1 year posttreatment, and 43 patients had a third biopsy after [5 years of treatment. Serum HBcrAg, HBV DNA and hepatitis B surface antigen (HBsAg), and intrahepatic cccDNA were measured. RESULTS: HBcrAg strongly correlated with cccDNA (r = 0.695), serum HBV DNA (r = 0.691), and HBsAg (r = 0.449; all p\0.00001). Serum HBV DNA were undetectable (\20 IU/mL) in 120 samples. In these 120 samples, HBcrAg also correlated positively with cccDNA (r = 0.388, p = 0.00001). At baseline, all 124 patients had HBcrAg[1 kU/mL (median level: 3.6 log kU/mL). At year 1 and [5 years post-treatment, the median HBcrAg levels were 2.3 and 0.77 log kU/mL, with a median logarithmic reduction of 1.4 and 2.7 log kU/mL (compared with baseline), respectively. 21 patients had undetectable cccDNA (\0.005 copies/cell) after[5 years post-treatment, in which 15 (71 %) had HBcrAg levels [1 kU/mL (range 1.2–537 kU/mL). CONCLUSIONS: Serum HBcrAg might be a potentially reliable surrogate marker for intrahepatic cccDNA content even when serum HBV DNA became undetectable. Viral proteins were still synthesized in the majority of patients with undetectable cccDNA after long-term treatment.
DescriptionThis journal suppl. entitled: Conference Abstracts: 25th Annual Conference of APASL, February 20–24, 2016, Tokyo, Japan
Oral Presentation: O-097
Persistent Identifierhttp://hdl.handle.net/10722/234170
ISSN
2015 Impact Factor: 1.125
2015 SCImago Journal Rankings: 0.669

 

DC FieldValueLanguage
dc.contributor.authorWong, DKH-
dc.contributor.authorSeto, WKW-
dc.contributor.authorHuang, FY-
dc.contributor.authorFung, JYY-
dc.contributor.authorLai, CL-
dc.contributor.authorYuen, RMF-
dc.date.accessioned2016-10-14T06:59:31Z-
dc.date.available2016-10-14T06:59:31Z-
dc.date.issued2016-
dc.identifier.citationThe 25th Annual Conference of Asian Pacific Association for the Study of the Liver (APASL 2016), Tokyo, Japan, 20-24 February 2016. In Hepatology International, 2016, v. 10 n. 1 suppl., p. S42, abstract no. O-097-
dc.identifier.issn1936-0533-
dc.identifier.urihttp://hdl.handle.net/10722/234170-
dc.descriptionThis journal suppl. entitled: Conference Abstracts: 25th Annual Conference of APASL, February 20–24, 2016, Tokyo, Japan-
dc.descriptionOral Presentation: O-097-
dc.description.abstractBACKGROUND: Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) is a key to viral persistence in chronic hepatitis B patients. We aimed to explore whether serum hepatitis B core-related antigen (HBcrAg) could be a surrogate marker for intrahepatic cccDNA in a large cohort of patients with liver biopsies. METHODS: 305 liver biopsies and the corresponding sera collected from 138 nucleoside/tide analogues-treated patients were analyzed. 124 patients had paired liver biopsies at baseline and 1 year posttreatment, and 43 patients had a third biopsy after [5 years of treatment. Serum HBcrAg, HBV DNA and hepatitis B surface antigen (HBsAg), and intrahepatic cccDNA were measured. RESULTS: HBcrAg strongly correlated with cccDNA (r = 0.695), serum HBV DNA (r = 0.691), and HBsAg (r = 0.449; all p\0.00001). Serum HBV DNA were undetectable (\20 IU/mL) in 120 samples. In these 120 samples, HBcrAg also correlated positively with cccDNA (r = 0.388, p = 0.00001). At baseline, all 124 patients had HBcrAg[1 kU/mL (median level: 3.6 log kU/mL). At year 1 and [5 years post-treatment, the median HBcrAg levels were 2.3 and 0.77 log kU/mL, with a median logarithmic reduction of 1.4 and 2.7 log kU/mL (compared with baseline), respectively. 21 patients had undetectable cccDNA (\0.005 copies/cell) after[5 years post-treatment, in which 15 (71 %) had HBcrAg levels [1 kU/mL (range 1.2–537 kU/mL). CONCLUSIONS: Serum HBcrAg might be a potentially reliable surrogate marker for intrahepatic cccDNA content even when serum HBV DNA became undetectable. Viral proteins were still synthesized in the majority of patients with undetectable cccDNA after long-term treatment.-
dc.languageeng-
dc.publisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/west/home/medicine?SGWID=4-10054-70-173733513-0-
dc.relation.ispartofHepatology International-
dc.titleHepatitis B virus core-related antigen as a surrogate marker for covalently closed circular DNA-
dc.typeConference_Paper-
dc.identifier.emailWong, DKH: danywong@hku.hk-
dc.identifier.emailSeto, WKW: wkseto@hku.hk-
dc.identifier.emailHuang, FY: fungyu@hkucc.hku.hk-
dc.identifier.emailFung, JYY: jfung@hkucc.hku.hk-
dc.identifier.emailLai, CL: hrmelcl@hkucc.hku.hk-
dc.identifier.emailYuen, RMF: mfyuen@hku.hk-
dc.identifier.authorityWong, DKH=rp00492-
dc.identifier.authoritySeto, WKW=rp01659-
dc.identifier.authorityFung, JYY=rp00518-
dc.identifier.authorityLai, CL=rp00314-
dc.identifier.authorityYuen, RMF=rp00479-
dc.identifier.doi10.1007/s12072-016-9707-8-
dc.identifier.hkuros267635-
dc.identifier.volume10-
dc.identifier.issue1 suppl.-
dc.identifier.spageS42, abstract no. O-097-
dc.identifier.epageS42, abstract no. O-097-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats