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Conference Paper: Neuroprotection of melatonin and/or calpeptin in a rat experimental cerebral ischaemia and reperfusion model

TitleNeuroprotection of melatonin and/or calpeptin in a rat experimental cerebral ischaemia and reperfusion model
Authors
Issue Date2016
PublisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org/
Citation
The 21st Medical Research Conference (MRC 2016), Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, 16 January 2016. In Hong Kong Medical Journal, 2016, v. 22 suppl. 1, p. 22, abstract no. 25 How to Cite?
AbstractINTRODUCTION: Melatonin is a potent antioxidant. Previously, we have demonstrated beneficial effects of pretreatment with melatonin in rodent models of focal cerebral ischaemia. Cerebral ischaemia increases intracellular concentration of calcium ion and activates several calcium-dependent proteases such as calpain. Calpeptin is a novel calpain inhibitor. The aim of this study was to investigate the neuroprotective effects of melatonin and/or calpeptin administered after onset of reperfusion in transient focal cerebral ischaemia. METHODS: Right-side middle cerebral artery occlusion was induced for 90 minutes in male Sprague Dawley rats weighing 260 to 280 g, and this was followed by reperfusion for 24 or 72 hours. Post-ischaemic treatment was delivered via an intracerebroventricular injection initiated about 10 to 15 minutes after the onset of the reperfusion. Regional cerebral blood flow was monitored using a laser Doppler flowmeter. Cerebral infarction volume was evaluated using tetrazolium staining and analysed by Image J software. Neurological behaviour was assessed using neurological deficit scoring system (NDSS). Protein for Western blot was extracted from brain tissue specimens obtained in the striatum and cortex. Coronal cryosections of 30 µm thick were collected from the brain, and three levels were selected for immunofluorescence or Fluoro-Jade to study neuronal survival. RESULTS: Three single doses of calpeptin, two single doses of melatonin, and two combination dosages were investigated. Treatment with either melatonin or calpeptin reduced infarction volume and NDSS score in a dose-dependent manner. Although calpeptin at 15 µg/kg and melatonin at 50 µg/kg did not show significant benefits when injected individually, the combination of the two treatments reduced the infarct volume and improved the neurological deficit at 24 hours after reperfusion. CONCLUSION: We conclude that the combination of melatonin and calpeptin exerts synergistic effects.
DescriptionPoster
Persistent Identifierhttp://hdl.handle.net/10722/232498
ISSN
2015 Impact Factor: 0.887
2015 SCImago Journal Rankings: 0.279

 

DC FieldValueLanguage
dc.contributor.authorFeng, Y-
dc.contributor.authorCheung, RTF-
dc.date.accessioned2016-09-20T05:30:26Z-
dc.date.available2016-09-20T05:30:26Z-
dc.date.issued2016-
dc.identifier.citationThe 21st Medical Research Conference (MRC 2016), Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, 16 January 2016. In Hong Kong Medical Journal, 2016, v. 22 suppl. 1, p. 22, abstract no. 25-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/232498-
dc.descriptionPoster-
dc.description.abstractINTRODUCTION: Melatonin is a potent antioxidant. Previously, we have demonstrated beneficial effects of pretreatment with melatonin in rodent models of focal cerebral ischaemia. Cerebral ischaemia increases intracellular concentration of calcium ion and activates several calcium-dependent proteases such as calpain. Calpeptin is a novel calpain inhibitor. The aim of this study was to investigate the neuroprotective effects of melatonin and/or calpeptin administered after onset of reperfusion in transient focal cerebral ischaemia. METHODS: Right-side middle cerebral artery occlusion was induced for 90 minutes in male Sprague Dawley rats weighing 260 to 280 g, and this was followed by reperfusion for 24 or 72 hours. Post-ischaemic treatment was delivered via an intracerebroventricular injection initiated about 10 to 15 minutes after the onset of the reperfusion. Regional cerebral blood flow was monitored using a laser Doppler flowmeter. Cerebral infarction volume was evaluated using tetrazolium staining and analysed by Image J software. Neurological behaviour was assessed using neurological deficit scoring system (NDSS). Protein for Western blot was extracted from brain tissue specimens obtained in the striatum and cortex. Coronal cryosections of 30 µm thick were collected from the brain, and three levels were selected for immunofluorescence or Fluoro-Jade to study neuronal survival. RESULTS: Three single doses of calpeptin, two single doses of melatonin, and two combination dosages were investigated. Treatment with either melatonin or calpeptin reduced infarction volume and NDSS score in a dose-dependent manner. Although calpeptin at 15 µg/kg and melatonin at 50 µg/kg did not show significant benefits when injected individually, the combination of the two treatments reduced the infarct volume and improved the neurological deficit at 24 hours after reperfusion. CONCLUSION: We conclude that the combination of melatonin and calpeptin exerts synergistic effects.-
dc.languageeng-
dc.publisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org/-
dc.relation.ispartofHong Kong Medical Journal-
dc.rightsHong Kong Medical Journal. Copyright © Hong Kong Academy of Medicine Press.-
dc.titleNeuroprotection of melatonin and/or calpeptin in a rat experimental cerebral ischaemia and reperfusion model-
dc.typeConference_Paper-
dc.identifier.emailCheung, RTF: rtcheung@hkucc.hku.hk-
dc.identifier.authorityCheung, RTF=rp00434-
dc.identifier.hkuros266701-
dc.identifier.volume22-
dc.identifier.issuesuppl. 1-
dc.identifier.spage22, abstract no. 25-
dc.identifier.epage22, abstract no. 25-
dc.publisher.placeHong Kong-

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