File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)

Conference Paper: Contact-dependent virulence of P. aeruginosa is required for elevating paracellular conductance of cultured pig tracheal epithelia

TitleContact-dependent virulence of P. aeruginosa is required for elevating paracellular conductance of cultured pig tracheal epithelia
Authors
Issue Date2015
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/39249
Citation
The 29th Annual Conference of the North American Cystic Fibrosis (NACF 2015), Phoenix, AZ., 8-10 October 2015. In Pediatric Pulmonology, 2015, v. 50 suppl. 41, abstract no. 125 How to Cite?
AbstractPseudomonas aeruginosa is a common opportunistic pathogen causing severe lung diseases in patients with cystic fibrosis or other airway disorders. In airway, epithelial tight junctions and ion transport are important for defending bacterial infection, whereas it is unclear whether P. aeruginosa modulates these epithelial properties for facilitating its invasion. To address this question, we first characterized our P. aeruginosa ATCC 28573 by a linear relationship between the number of bacteria and OD600 absorbance, doubling time of about 58 minutes for bacterial growth and sequencing specific genes including oprL, ExoA and 16S rDNA. Next, P. aeruginosa in Krebs solution was inoculated to primary culture of pig tracheal epithelia at the multiplicity of infection (MOI) of 0.1, 10 or 200 for 1, 4, 7 and 10 hours. Our Ussing chamber study demonstrates that the basal short-circuit currents (Isc) and Isc changes by different stimulators and inhibitors of ion transporters were not apparently altered by P. aeruginosa inoculation in all MOI groups from 1 to 10 hours, compared to that of the control Krebs solution. However, in all MOI groups tested, the transepithelial conductance (Gt) at basal (Gt-basal) and at the end of experiment (Gt-end) of cultured epithelia were not altered at 1 hour of bacterial inoculation, but slightly increased at 4 hours and greatly elevated at both 7 and 10 hours, compared to that of the control. These data suggest that in cultured epithelia, the tight junctions were loosened by apical P. aeruginosa inoculation in a time-dependent manner. To explore the origin of bacterial toxins that increased Gt, cultured epithelia were inoculated with UV-inactivated P. aeruginosa at MOI = 200 or with the supernatant solution collected from the bacterial solution after 7 hours incubation of P. aeruginosa at MOI = 1000. The data demonstrate that Gt-basal and Gt-end of cultured pig tracheal epithelia were greatly increased by UV-inactivated bacteria but not altered by the supernatant solution. These data suggest that the interactions of bacteria with the epithelial cells may be required for initiating the Gt increase of cultured pig tracheal epithelia. From these results taken together, we speculate that by attaching to the epithelial cells, P. aeruginosa disrupts the assembly of tight junctions at an early stage of infection for the purpose of grabbing nutrients from the mucosal layer or traversing through the epithelial barrier.
DescriptionPoster Session: no. 125
This journal suppl. entitled: Special Issue: The 29 Annual North American Cystic Fibrosis Conference October 8-10, 2015
Persistent Identifierhttp://hdl.handle.net/10722/231470
ISSN
2015 Impact Factor: 2.85
2015 SCImago Journal Rankings: 0.910

 

DC FieldValueLanguage
dc.contributor.authorXU, W-
dc.contributor.authorLi, NF-
dc.contributor.authorChen, J-
dc.date.accessioned2016-09-20T05:23:21Z-
dc.date.available2016-09-20T05:23:21Z-
dc.date.issued2015-
dc.identifier.citationThe 29th Annual Conference of the North American Cystic Fibrosis (NACF 2015), Phoenix, AZ., 8-10 October 2015. In Pediatric Pulmonology, 2015, v. 50 suppl. 41, abstract no. 125-
dc.identifier.issn8755-6863-
dc.identifier.urihttp://hdl.handle.net/10722/231470-
dc.descriptionPoster Session: no. 125-
dc.descriptionThis journal suppl. entitled: Special Issue: The 29 Annual North American Cystic Fibrosis Conference October 8-10, 2015-
dc.description.abstractPseudomonas aeruginosa is a common opportunistic pathogen causing severe lung diseases in patients with cystic fibrosis or other airway disorders. In airway, epithelial tight junctions and ion transport are important for defending bacterial infection, whereas it is unclear whether P. aeruginosa modulates these epithelial properties for facilitating its invasion. To address this question, we first characterized our P. aeruginosa ATCC 28573 by a linear relationship between the number of bacteria and OD600 absorbance, doubling time of about 58 minutes for bacterial growth and sequencing specific genes including oprL, ExoA and 16S rDNA. Next, P. aeruginosa in Krebs solution was inoculated to primary culture of pig tracheal epithelia at the multiplicity of infection (MOI) of 0.1, 10 or 200 for 1, 4, 7 and 10 hours. Our Ussing chamber study demonstrates that the basal short-circuit currents (Isc) and Isc changes by different stimulators and inhibitors of ion transporters were not apparently altered by P. aeruginosa inoculation in all MOI groups from 1 to 10 hours, compared to that of the control Krebs solution. However, in all MOI groups tested, the transepithelial conductance (Gt) at basal (Gt-basal) and at the end of experiment (Gt-end) of cultured epithelia were not altered at 1 hour of bacterial inoculation, but slightly increased at 4 hours and greatly elevated at both 7 and 10 hours, compared to that of the control. These data suggest that in cultured epithelia, the tight junctions were loosened by apical P. aeruginosa inoculation in a time-dependent manner. To explore the origin of bacterial toxins that increased Gt, cultured epithelia were inoculated with UV-inactivated P. aeruginosa at MOI = 200 or with the supernatant solution collected from the bacterial solution after 7 hours incubation of P. aeruginosa at MOI = 1000. The data demonstrate that Gt-basal and Gt-end of cultured pig tracheal epithelia were greatly increased by UV-inactivated bacteria but not altered by the supernatant solution. These data suggest that the interactions of bacteria with the epithelial cells may be required for initiating the Gt increase of cultured pig tracheal epithelia. From these results taken together, we speculate that by attaching to the epithelial cells, P. aeruginosa disrupts the assembly of tight junctions at an early stage of infection for the purpose of grabbing nutrients from the mucosal layer or traversing through the epithelial barrier.-
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/39249-
dc.relation.ispartofPediatric Pulmonology-
dc.rightsPediatric Pulmonology. Copyright © John Wiley & Sons, Inc.-
dc.rightsPreprint: This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Postprint: This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Special Statement for Preprint only Before publication: 'This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright © ([year]) ([Pathological Society of Great Britain and Ireland])'. After publication: the preprint notice should be amended to follows: 'This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]' For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement & acknowledgement : ‘This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.’ Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX) (This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.)-
dc.titleContact-dependent virulence of P. aeruginosa is required for elevating paracellular conductance of cultured pig tracheal epithelia-
dc.typeConference_Paper-
dc.identifier.emailLi, NF: nfli15@hku.hk-
dc.identifier.emailChen, J: jeng-haur-chen@hku.hk-
dc.identifier.authorityChen, J=rp01518-
dc.identifier.doi10.1002/ppul.23297-
dc.identifier.hkuros264098-
dc.identifier.volume50-
dc.identifier.issuesuppl. 41-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats