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Conference Paper: Probing the membrane characteristics and long-term volumetric response of live cells

TitleProbing the membrane characteristics and long-term volumetric response of live cells
Authors
Issue Date2013
Citation
The SES 50th Annual Technical Meeting and ASME-AMD Annual Summer Meeting (SES 2013), Providence, RI., 28-31 July 2013. How to Cite?
AbstractCell regulations involve exchange of ion species and water molecules across the cell membrane, and are important in processes including programmed cell death (PCD), regulatory volume decrease (RVD) or increase (RVI). Probing such membrane transport processes requires volumetric changes of the cell, but all existing single cell measurement methods preserve the volume. In this work, we develop a novel method to introduce precise size changes of cells by controlling the medium pressure mechanically or osmotically. In particular, the pressure-induced shrinking/swelling of K562 and HL60 cell lines is systematically examined from which the water permeability and stretching rigidity of the membrane of these cells are obtained. We also find that the characteristic time associated with volumetric deformation of live cells, treated as a bulk material, is of the order of ~1 hour, an information that is missing from other testing methods but could be critical in our understanding of how slow processes like tissue growth or tumor development take place.
DescriptionSession - Mechanics of Biological and Soft Materials: Mechanics of cell sheets, multicellular assemblies and tissues
Persistent Identifierhttp://hdl.handle.net/10722/229823

 

DC FieldValueLanguage
dc.contributor.authorHui, TH-
dc.contributor.authorLin, Y-
dc.contributor.authorNgan, AHW-
dc.date.accessioned2016-08-23T14:13:27Z-
dc.date.available2016-08-23T14:13:27Z-
dc.date.issued2013-
dc.identifier.citationThe SES 50th Annual Technical Meeting and ASME-AMD Annual Summer Meeting (SES 2013), Providence, RI., 28-31 July 2013.-
dc.identifier.urihttp://hdl.handle.net/10722/229823-
dc.descriptionSession - Mechanics of Biological and Soft Materials: Mechanics of cell sheets, multicellular assemblies and tissues-
dc.description.abstractCell regulations involve exchange of ion species and water molecules across the cell membrane, and are important in processes including programmed cell death (PCD), regulatory volume decrease (RVD) or increase (RVI). Probing such membrane transport processes requires volumetric changes of the cell, but all existing single cell measurement methods preserve the volume. In this work, we develop a novel method to introduce precise size changes of cells by controlling the medium pressure mechanically or osmotically. In particular, the pressure-induced shrinking/swelling of K562 and HL60 cell lines is systematically examined from which the water permeability and stretching rigidity of the membrane of these cells are obtained. We also find that the characteristic time associated with volumetric deformation of live cells, treated as a bulk material, is of the order of ~1 hour, an information that is missing from other testing methods but could be critical in our understanding of how slow processes like tissue growth or tumor development take place.-
dc.languageeng-
dc.relation.ispartofSES Annual Technical Meeting and ASME-AMD Annual Summer Meeting, SES 2013-
dc.titleProbing the membrane characteristics and long-term volumetric response of live cells-
dc.typeConference_Paper-
dc.identifier.emailLin, Y: ylin@hkucc.hku.hk-
dc.identifier.emailNgan, AHW: hwngan@hku.hk-
dc.identifier.authorityLin, Y=rp00080-
dc.identifier.authorityNgan, AHW=rp00225-
dc.identifier.hkuros261887-

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