File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Material- and feature-dependent effects on cell adhesion to micro injection moulded medical polymers

TitleMaterial- and feature-dependent effects on cell adhesion to micro injection moulded medical polymers
Authors
KeywordsCyclic olefin copolymer (COC)
Issue Date2016
Citation
Colloids and Surfaces B: Biointerfaces, 2016, v. 145, p. 46-54 How to Cite?
Abstract© 2016 Elsevier B.V.Two polymers, polymethylmethacrylate (PMMA) and cyclic olefin copolymer (COC), containing a range of nano- to micron- roughness surfaces (Ra 0.01, 0.1, 0.4, 1.0, 2.0, 3.2 and 5.0 μm) were fabricated using electrical discharge machining (EDM) and replicated using micro injection moulding (μIM). Polymer samples were characterized using optical profilometry, atomic force microscopy (AFM) and water surface contact angle. Cell adhesion tests were carried out using bacterial Pseudomonas fluorescens and mammalian Madin-Darby Canine Kidney (MDCK) cells to determine the effect of surface hydrophobicity, surface roughness and stiffness. It is found that there are features which gave insignificant differences (feature-dependent effect) in cell adhesion, albeit a significant difference in the physicochemical properties (material-dependent effect) of substrata. In bacterial cell adhesion, the strongest feature-dependence is found at Ra 0.4 μm surfaces, with material-dependent effects strongest at Ra 0.01 μm. Ra 0.1 μm surfaces exhibited strongest feature-dependent effects and Ra 5.0 μm has strongest material-dependent effects on mammalian cell adhesion. Bacterial cell adhesion is found to be favourable to hydrophobic surfaces (COC), with the lowest adhesion at Ra 0.4 μm for both materials. Mammalian cell adhesion is lowest in Ra 0.1 μm and highest in Ra 1.0 μm, and generally favours hydrophilic surfaces (PMMA). These findings can be used as a basis for developing medical implants or microfluidic devices using micro injection moulding for diagnostic purposes, by tuning the cell adhesion on different areas containing different surface roughnesses on the diagnostic microfluidic devices or medical implants.
Persistent Identifierhttp://hdl.handle.net/10722/228246
ISSN
2015 Impact Factor: 3.902
2015 SCImago Journal Rankings: 1.105

 

DC FieldValueLanguage
dc.contributor.authorChoi, Seong Ying-
dc.contributor.authorHabimana, Olivier-
dc.contributor.authorFlood, Peter-
dc.contributor.authorReynaud, Emmanuel G.-
dc.contributor.authorRodriguez, Brian J.-
dc.contributor.authorZhang, Nan-
dc.contributor.authorCasey, Eoin-
dc.contributor.authorGilchrist, Michael D.-
dc.date.accessioned2016-08-01T06:45:33Z-
dc.date.available2016-08-01T06:45:33Z-
dc.date.issued2016-
dc.identifier.citationColloids and Surfaces B: Biointerfaces, 2016, v. 145, p. 46-54-
dc.identifier.issn0927-7765-
dc.identifier.urihttp://hdl.handle.net/10722/228246-
dc.description.abstract© 2016 Elsevier B.V.Two polymers, polymethylmethacrylate (PMMA) and cyclic olefin copolymer (COC), containing a range of nano- to micron- roughness surfaces (Ra 0.01, 0.1, 0.4, 1.0, 2.0, 3.2 and 5.0 μm) were fabricated using electrical discharge machining (EDM) and replicated using micro injection moulding (μIM). Polymer samples were characterized using optical profilometry, atomic force microscopy (AFM) and water surface contact angle. Cell adhesion tests were carried out using bacterial Pseudomonas fluorescens and mammalian Madin-Darby Canine Kidney (MDCK) cells to determine the effect of surface hydrophobicity, surface roughness and stiffness. It is found that there are features which gave insignificant differences (feature-dependent effect) in cell adhesion, albeit a significant difference in the physicochemical properties (material-dependent effect) of substrata. In bacterial cell adhesion, the strongest feature-dependence is found at Ra 0.4 μm surfaces, with material-dependent effects strongest at Ra 0.01 μm. Ra 0.1 μm surfaces exhibited strongest feature-dependent effects and Ra 5.0 μm has strongest material-dependent effects on mammalian cell adhesion. Bacterial cell adhesion is found to be favourable to hydrophobic surfaces (COC), with the lowest adhesion at Ra 0.4 μm for both materials. Mammalian cell adhesion is lowest in Ra 0.1 μm and highest in Ra 1.0 μm, and generally favours hydrophilic surfaces (PMMA). These findings can be used as a basis for developing medical implants or microfluidic devices using micro injection moulding for diagnostic purposes, by tuning the cell adhesion on different areas containing different surface roughnesses on the diagnostic microfluidic devices or medical implants.-
dc.languageeng-
dc.relation.ispartofColloids and Surfaces B: Biointerfaces-
dc.subjectCyclic olefin copolymer (COC)-
dc.titleMaterial- and feature-dependent effects on cell adhesion to micro injection moulded medical polymers-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.colsurfb.2016.04.032-
dc.identifier.scopuseid_2-s2.0-84964430196-
dc.identifier.volume145-
dc.identifier.spage46-
dc.identifier.epage54-
dc.identifier.eissn1873-4367-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats