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Article: Microencapsulation of Neuroblastoma Cells and Mesenchymal Stromal Cells in Collagen Microspheres: A 3D Model for Cancer Cell Niche Study

TitleMicroencapsulation of Neuroblastoma Cells and Mesenchymal Stromal Cells in Collagen Microspheres: A 3D Model for Cancer Cell Niche Study
Authors
Issue Date2015
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2015, v. 10 n. 12, p. e0144139 How to Cite?
AbstractThere is a growing trend for researchers to use in vitro 3D models in cancer studies, as they can better recapitulate the complex in vivo situation. And the fact that the progression and development of tumor are closely associated to its stromal microenvironment has been increasingly recognized. The establishment of such tumor supportive niche is vital in understanding tumor progress and metastasis. The mesenchymal origin of many cells residing in the cancer niche provides the rationale to include MSCs in mimicking the niche in neuroblastoma. Here we co-encapsulate and co-culture NBCs and MSCs in a 3D in vitro model and investigate the morphology, growth kinetics and matrix remodeling in the reconstituted stromal environment. Results showed that the incorporation of MSCs in the model lead to accelerated growth of cancer cells as well as recapitulation of at least partially the tumor microenvironment in vivo. The current study therefore demonstrates the feasibility for the collagen microsphere to act as a 3D in vitro cancer model for various topics in cancer studies.
Persistent Identifierhttp://hdl.handle.net/10722/227873
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYeung, P-
dc.contributor.authorSin, HS-
dc.contributor.authorChan, S-
dc.contributor.authorChan, GCF-
dc.contributor.authorChan, BP-
dc.date.accessioned2016-07-21T06:30:33Z-
dc.date.available2016-07-21T06:30:33Z-
dc.date.issued2015-
dc.identifier.citationPlos One, 2015, v. 10 n. 12, p. e0144139-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/227873-
dc.description.abstractThere is a growing trend for researchers to use in vitro 3D models in cancer studies, as they can better recapitulate the complex in vivo situation. And the fact that the progression and development of tumor are closely associated to its stromal microenvironment has been increasingly recognized. The establishment of such tumor supportive niche is vital in understanding tumor progress and metastasis. The mesenchymal origin of many cells residing in the cancer niche provides the rationale to include MSCs in mimicking the niche in neuroblastoma. Here we co-encapsulate and co-culture NBCs and MSCs in a 3D in vitro model and investigate the morphology, growth kinetics and matrix remodeling in the reconstituted stromal environment. Results showed that the incorporation of MSCs in the model lead to accelerated growth of cancer cells as well as recapitulation of at least partially the tumor microenvironment in vivo. The current study therefore demonstrates the feasibility for the collagen microsphere to act as a 3D in vitro cancer model for various topics in cancer studies.-
dc.languageeng-
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPlos One-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleMicroencapsulation of Neuroblastoma Cells and Mesenchymal Stromal Cells in Collagen Microspheres: A 3D Model for Cancer Cell Niche Study-
dc.typeArticle-
dc.identifier.emailChan, S: schan88@hkucc.hku.hk-
dc.identifier.emailChan, GCF: gcfchan@hkucc.hku.hk-
dc.identifier.emailChan, BP: bpchan@hkucc.hku.hk-
dc.identifier.authorityChan, GCF=rp00431-
dc.identifier.authorityChan, BP=rp00087-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0144139-
dc.identifier.pmid26657086-
dc.identifier.volume10-
dc.identifier.issue12-
dc.identifier.spagee0144139-
dc.identifier.epagee0144139-
dc.identifier.isiWOS:000366715900028-
dc.publisher.placeUnited States-

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