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Conference Paper: Endothelin-1 overexpression exacerbates experimental autoimmune encephalomyelitis

TitleEndothelin-1 overexpression exacerbates experimental autoimmune encephalomyelitis
Authors
Issue Date2015
PublisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org/
Citation
The 20th Medical Research Conference, Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, 17 January 2015. In Hong Kong Medical Journal, 2015, v. 21 suppl. 1, p. 22, abstract no. 25 How to Cite?
AbstractMultiple sclerosis (MS) is a central nervous system inflammatory demyelinating disorder. T helper 1 (Th1) and T helper 17 (Th17) cells are important in MS immunopathogenesis. Level of endothelin-1 (ET-1), a vasoconstrictor, is increased in sera of MS patients. We studied the role of ET-1 in experimental allergic encephalomyelitis (EAE), a MS animal model. EAE was induced in transgenic mice overexpressing endothelial ET-1 (TET-1), transgenic mice overexpressing astrocytic ET-1 (GET-1) and non-transgenic (NTg) mice by immunisation with myelin oligodendrocyte glycoprotein (MOG)35-55 peptide. EAE scores, spinal cord histology, serum proinflammatory cytokines levels, and proinflammatory cytokines production from splenocytes of ET-1 transgenic and NTg mice with EAE were studied. ET-1 transgenic mice developed more severe EAE than NTg with increased inflammation and demyelination in spinal cord. The mean maximum EAE scores for GET-1, TET-1 and NTg mice with EAE were 4.84, 4.31 and 4.05 respectively (P<0.05). Serum levels of interleukin (IL)–6, IL-17A, interferon (IFN)–γ and tumour necrosis factor (TNF)–α were higher in ET-1 transgenic than NTg mice with EAE (P<0.05) while serum IL-4 levels were similar. mRNA levels of IL-6, IL-17A, IFN-γ and TNF-α from cultured splenocytes were higher in ET-1-transgenic than NTg mice with EAE (P<0.05), while IL-4 mRNA levels were similar. Consistently, levels of IL-6, IL-17A, IFN-γ and TNF-α in culture media of splenocytes were higher in ET-1 transgenic than NTg mice with EAE (P<0.05), while IL-4 levels were similar. We concluded that mice with endothelial or astrocytic ET-1 overexpression developed more severe EAE with increased splenic lymphocytes production of Th1 and Th17 proinflammatory cytokines.
Persistent Identifierhttp://hdl.handle.net/10722/227518
ISSN
2015 Impact Factor: 0.887
2015 SCImago Journal Rankings: 0.279

 

DC FieldValueLanguage
dc.contributor.authorGuo, VY-
dc.contributor.authorChung, SK-
dc.contributor.authorSiu, DCW-
dc.contributor.authorKwan, JSK-
dc.contributor.authorHo, PWL-
dc.contributor.authorLee, JCY-
dc.contributor.authorYeung, PKK-
dc.contributor.authorChan, KH-
dc.date.accessioned2016-07-18T09:11:11Z-
dc.date.available2016-07-18T09:11:11Z-
dc.date.issued2015-
dc.identifier.citationThe 20th Medical Research Conference, Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, 17 January 2015. In Hong Kong Medical Journal, 2015, v. 21 suppl. 1, p. 22, abstract no. 25-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/227518-
dc.description.abstractMultiple sclerosis (MS) is a central nervous system inflammatory demyelinating disorder. T helper 1 (Th1) and T helper 17 (Th17) cells are important in MS immunopathogenesis. Level of endothelin-1 (ET-1), a vasoconstrictor, is increased in sera of MS patients. We studied the role of ET-1 in experimental allergic encephalomyelitis (EAE), a MS animal model. EAE was induced in transgenic mice overexpressing endothelial ET-1 (TET-1), transgenic mice overexpressing astrocytic ET-1 (GET-1) and non-transgenic (NTg) mice by immunisation with myelin oligodendrocyte glycoprotein (MOG)35-55 peptide. EAE scores, spinal cord histology, serum proinflammatory cytokines levels, and proinflammatory cytokines production from splenocytes of ET-1 transgenic and NTg mice with EAE were studied. ET-1 transgenic mice developed more severe EAE than NTg with increased inflammation and demyelination in spinal cord. The mean maximum EAE scores for GET-1, TET-1 and NTg mice with EAE were 4.84, 4.31 and 4.05 respectively (P<0.05). Serum levels of interleukin (IL)–6, IL-17A, interferon (IFN)–γ and tumour necrosis factor (TNF)–α were higher in ET-1 transgenic than NTg mice with EAE (P<0.05) while serum IL-4 levels were similar. mRNA levels of IL-6, IL-17A, IFN-γ and TNF-α from cultured splenocytes were higher in ET-1-transgenic than NTg mice with EAE (P<0.05), while IL-4 mRNA levels were similar. Consistently, levels of IL-6, IL-17A, IFN-γ and TNF-α in culture media of splenocytes were higher in ET-1 transgenic than NTg mice with EAE (P<0.05), while IL-4 levels were similar. We concluded that mice with endothelial or astrocytic ET-1 overexpression developed more severe EAE with increased splenic lymphocytes production of Th1 and Th17 proinflammatory cytokines.-
dc.languageeng-
dc.publisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org/-
dc.relation.ispartofHong Kong Medical Journal-
dc.rightsHong Kong Medical Journal. Copyright © Hong Kong Academy of Medicine Press.-
dc.titleEndothelin-1 overexpression exacerbates experimental autoimmune encephalomyelitis-
dc.typeConference_Paper-
dc.identifier.emailGuo, VY: viviguo@hku.hk-
dc.identifier.emailChung, SK: skchung@hkucc.hku.hk-
dc.identifier.emailSiu, DCW: cwdsiu@hkucc.hku.hk-
dc.identifier.emailKwan, JSK: jskkwan@hku.hk-
dc.identifier.emailHo, PWL: hwl2002@hku.hk-
dc.identifier.emailYeung, PKK: ykkp@hkucc.hku.hk-
dc.identifier.emailChan, KH: koonho@hku.hk-
dc.identifier.authorityChung, SK=rp00381-
dc.identifier.authoritySiu, DCW=rp00534-
dc.identifier.authorityKwan, JSK=rp01868-
dc.identifier.authorityHo, PWL=rp00259-
dc.identifier.authorityChan, KH=rp00537-
dc.identifier.hkuros258976-
dc.identifier.volume21-
dc.identifier.issuesuppl. 1-
dc.identifier.spage22, abstract no. 25-
dc.identifier.epage22, abstract no. 25-
dc.publisher.placeHong Kong-

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