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Conference Paper: IL-17 expression and its effect on osteoclastogenesis under mechanical loading

TitleIL-17 expression and its effect on osteoclastogenesis under mechanical loading
Authors
Issue Date2016
PublisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/
Citation
The 94th General Session & Exhibition of the IADR, 3rd Meeting of the IADR Asia Pacific Region & 35th Annual Meeting of the IADR Korean Division, Seoul, Korea, 22-25 June 2016. In Journal of Dental Research, 2016, v. 95 Spec. Iss. B, abstract no. 1666 How to Cite?
AbstractOBJECTIVES: The molecular mechanisms of bone remodeling under mechanical loading is crucial for understanding alveolar bone metabolism. Interleukin (IL)-17 plays a key role in orchestrate osteoclast activation and differentiation. This study investigated the expression of IL-17 under mechanical force and its effect on osteoclastogenesis in osteocyte-like cells. METHODS: MLO-Y4 osteocytes were loaded with flow shear stress driven by Streamer® Shear Stress Device (4 dyn/cm2 and 16 dyn/cm2 respectively, 0.5 Hz, 2h). IL-17 at 0.1, 1 and 10ng/ml stimulated the osteocytes for 24h. The IL-17, IL-17 receptor (IL-17R), osteoprotegerin (OPG) and the receptor activator of NF-κB ligand (RANKL) mRNA expressions were analyzed by real-time polymerase chain reaction (qPCR). Co-culture of osteocytes and bone marrow-derived macrophage cells (BMMs) was undertaken to investigate the effect of IL-17 on osteoclastogenesis. Conditional medium (CM) from MLO-Y4 was collected 24h after force loading. Cells were co-cultured in normal medium or CM with or without IL-17 for 7 days. Tartrate-resistant acid phosphatase (TRAP) staining cells were quantified. RESULTS: The basal expression of IL-17 and IL-17R in MLO-Y4 osteocytes was up-regulated under shear stress loading. IL-17 significantly enhanced the mRNA expression of RANKL while decreased the ratio of OPG/RANKL. In co-culture of MLO-Y4 and BMMs, IL-17 significantly increased the formation of TRAP+ osteoclasts, while the CM from shear stress loaded MLO-Y4 inhibited the stimulatory effect of IL-17 on osteoclastogenesis. CONCLUSIONS: Mechanical loading enhances the expression of IL-17 and IL-17R in MLO-Y4 osteocytes. IL-17 promotes osteoclastogenisis, whereas such effect seems to be weakened under shear stress stimulation. Further study on the exact underlying mechanisms is warranted.
DescriptionPoster Session - Biology of Tooth Movement: no. 1666
Persistent Identifierhttp://hdl.handle.net/10722/227497
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorYang, Y-
dc.contributor.authorLiao, C-
dc.contributor.authorJin, L-
dc.contributor.authorZhang, C-
dc.date.accessioned2016-07-18T09:11:04Z-
dc.date.available2016-07-18T09:11:04Z-
dc.date.issued2016-
dc.identifier.citationThe 94th General Session & Exhibition of the IADR, 3rd Meeting of the IADR Asia Pacific Region & 35th Annual Meeting of the IADR Korean Division, Seoul, Korea, 22-25 June 2016. In Journal of Dental Research, 2016, v. 95 Spec. Iss. B, abstract no. 1666-
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/227497-
dc.descriptionPoster Session - Biology of Tooth Movement: no. 1666-
dc.description.abstractOBJECTIVES: The molecular mechanisms of bone remodeling under mechanical loading is crucial for understanding alveolar bone metabolism. Interleukin (IL)-17 plays a key role in orchestrate osteoclast activation and differentiation. This study investigated the expression of IL-17 under mechanical force and its effect on osteoclastogenesis in osteocyte-like cells. METHODS: MLO-Y4 osteocytes were loaded with flow shear stress driven by Streamer® Shear Stress Device (4 dyn/cm2 and 16 dyn/cm2 respectively, 0.5 Hz, 2h). IL-17 at 0.1, 1 and 10ng/ml stimulated the osteocytes for 24h. The IL-17, IL-17 receptor (IL-17R), osteoprotegerin (OPG) and the receptor activator of NF-κB ligand (RANKL) mRNA expressions were analyzed by real-time polymerase chain reaction (qPCR). Co-culture of osteocytes and bone marrow-derived macrophage cells (BMMs) was undertaken to investigate the effect of IL-17 on osteoclastogenesis. Conditional medium (CM) from MLO-Y4 was collected 24h after force loading. Cells were co-cultured in normal medium or CM with or without IL-17 for 7 days. Tartrate-resistant acid phosphatase (TRAP) staining cells were quantified. RESULTS: The basal expression of IL-17 and IL-17R in MLO-Y4 osteocytes was up-regulated under shear stress loading. IL-17 significantly enhanced the mRNA expression of RANKL while decreased the ratio of OPG/RANKL. In co-culture of MLO-Y4 and BMMs, IL-17 significantly increased the formation of TRAP+ osteoclasts, while the CM from shear stress loaded MLO-Y4 inhibited the stimulatory effect of IL-17 on osteoclastogenesis. CONCLUSIONS: Mechanical loading enhances the expression of IL-17 and IL-17R in MLO-Y4 osteocytes. IL-17 promotes osteoclastogenisis, whereas such effect seems to be weakened under shear stress stimulation. Further study on the exact underlying mechanisms is warranted.-
dc.languageeng-
dc.publisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/-
dc.relation.ispartofJournal of Dental Research-
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.titleIL-17 expression and its effect on osteoclastogenesis under mechanical loading-
dc.typeConference_Paper-
dc.identifier.emailYang, Y: yangyanq@hku.hk-
dc.identifier.emailJin, L: ljjin@hkucc.hku.hk-
dc.identifier.emailZhang, C: zhangcf@hku.hk-
dc.identifier.authorityYang, Y=rp00045-
dc.identifier.authorityJin, L=rp00028-
dc.identifier.authorityZhang, C=rp01408-
dc.identifier.hkuros259740-
dc.identifier.volume95-
dc.identifier.issueSpec. Iss. B-
dc.publisher.placeUnited States-

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