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Article: Cloning of the black seabream (Acanthopagrus schlegeli) antiquitin gene and functional characterization of its promoter region

TitleCloning of the black seabream (Acanthopagrus schlegeli) antiquitin gene and functional characterization of its promoter region
Authors
KeywordsGene structure
Antiquitin
Black seabream
Promoter
Issue Date2007
Citation
Molecular and Cellular Biochemistry, 2007, v. 297, n. 1-2, p. 151-160 How to Cite?
AbstractAntiquitin (ALDH7) is a member of the aldehyde dehydrogenase superfamily. In plants, ALDH7 is inducible upon dehydration and is thus believed to possess an osmoregulatory role. On the other hand, however, its exact physiological function in animals remains elusive. We herein report the isolation of the black seabream (Acanthopagrus schlegeli) antiquitin gene (sbALDH7) and the functional characterization of its promoter region. The 1.6 kb 5′-flanking region of sbALDH7 exhibits an intense promoter activity (30-170 fold of the basal) in five mammalian and fish cell lines of different origins. Progressive 5′-deletion analysis suggests that the core promoter is located within the region -297/+41 whereas a cis-acting repressor of basal transcription is present in the region -878/-297. In silico analysis of this sbALDH7 promoter region does not reveal any osmotic response element. Instead, it contains potential binding sites for cell cycle related cis-elements such as CCAAT displacement protein and cell cycle-dependent element/cell cycle genes homology region. © Springer Science+Business Media, LLC 2006.
Persistent Identifierhttp://hdl.handle.net/10722/225032
ISSN
2015 Impact Factor: 2.613
2015 SCImago Journal Rankings: 1.019

 

DC FieldValueLanguage
dc.contributor.authorChan, Chi Bun-
dc.contributor.authorTang, Wai Kwan-
dc.contributor.authorCheng, Christopher H K-
dc.contributor.authorFong, Wing Ping-
dc.date.accessioned2016-04-18T11:16:34Z-
dc.date.available2016-04-18T11:16:34Z-
dc.date.issued2007-
dc.identifier.citationMolecular and Cellular Biochemistry, 2007, v. 297, n. 1-2, p. 151-160-
dc.identifier.issn0300-8177-
dc.identifier.urihttp://hdl.handle.net/10722/225032-
dc.description.abstractAntiquitin (ALDH7) is a member of the aldehyde dehydrogenase superfamily. In plants, ALDH7 is inducible upon dehydration and is thus believed to possess an osmoregulatory role. On the other hand, however, its exact physiological function in animals remains elusive. We herein report the isolation of the black seabream (Acanthopagrus schlegeli) antiquitin gene (sbALDH7) and the functional characterization of its promoter region. The 1.6 kb 5′-flanking region of sbALDH7 exhibits an intense promoter activity (30-170 fold of the basal) in five mammalian and fish cell lines of different origins. Progressive 5′-deletion analysis suggests that the core promoter is located within the region -297/+41 whereas a cis-acting repressor of basal transcription is present in the region -878/-297. In silico analysis of this sbALDH7 promoter region does not reveal any osmotic response element. Instead, it contains potential binding sites for cell cycle related cis-elements such as CCAAT displacement protein and cell cycle-dependent element/cell cycle genes homology region. © Springer Science+Business Media, LLC 2006.-
dc.languageeng-
dc.relation.ispartofMolecular and Cellular Biochemistry-
dc.subjectGene structure-
dc.subjectAntiquitin-
dc.subjectBlack seabream-
dc.subjectPromoter-
dc.titleCloning of the black seabream (Acanthopagrus schlegeli) antiquitin gene and functional characterization of its promoter region-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11010-006-9340-2-
dc.identifier.pmid17075685-
dc.identifier.scopuseid_2-s2.0-33947368151-
dc.identifier.volume297-
dc.identifier.issue1-2-
dc.identifier.spage151-
dc.identifier.epage160-
dc.identifier.eissn1573-4919-

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