Congenital Duodenal Atresia in Rat Model Induced by Adriamycin

Abstract

Objective To describe a reprducible fetal model of duedenal atresia. Methods Themated Pregnant rats were given 1. 75 rug/kg of Adriamycin intraperitoneally on days 6 through 9 of gestation, and the litters were recovered on day 20. The fetuses were dissected microscopically and studied histologically. The findings were compared with those of age-matched saline embryos. Results All saline embryos were normal, whereas 81. 7% (26/32) of Adriamycin ernbryoo had duodenal atresia. The intralumi-nal atresia was found in 15. 4% (4/26) of duedenal atresia embryos, gapped atresia with pancreatic tissuefilled in between in 46. 2% (12/26) and gapped atresia in 38. 5 % (10/26). A double duedenal atresia wasfound in one fetus. The malformation was anatomically identical to that of human human. Interestingly,all duedenal atresia embryco were saaociated with pancreatic agenesis, in 34. 6 % with the pancreatic neck,ho and tail absence and in 65. 4% with the body and tail absence. Conclusion This easily repnducibleexperimental model permits new research into both its embryogenesis and the biology if the duedenal atresiafetus.

目的制作阿霉素誘導大白鼠胎仔出現先天性十二指腸閉鎖的模型。方法Ｗｉｓｔａｒ孕鼠１０只，于妊娠第６至第９天腹腔注射阿霉素１７５ｍｐ／ｋｇ，妊娠２０天剖宮取胎仔，做解剖、電鏡及光鏡觀察。結果用藥組獲胎仔３２只，十二指腸閉鎖２６例（８１．３％），其中腔內閉鎖１５．４％，閉鎖近遠端由纖維或胰腺相連者占４６．２％，近遠端游離者為３８．５％，十二指腸閉鎖畸形均伴有胰腺發育異常，９例胰頸、胰體及胰尾缺如，１７例胰體和胰尾缺如，另外，鏡下見部分胰腺腺泡呈發育不良改變。結論阿霉素誘導大鼠胎仔出現先天性十二指腸畸形和胰腺畸形是一種可靠的模型制作方法。