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Conference Paper: Engineering Mineralised Tissue Pieces using Human Dental Pulp Cells

TitleEngineering Mineralised Tissue Pieces using Human Dental Pulp Cells
Authors
KeywordsBone
Bioengineering
Formation
Issue Date2010
PublisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/
Citation
The 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010. In Journal of Dental Research, 2010, v. 89 n. Spec Iss C, p. Abstract no.97 How to Cite?
AbstractObjective: This study investigates the possibility to produce small pieces of mineralised tissue for future clinical use. Methods: Cells were isolated from human dental pulps. After culture and expansion the cells were encapsulated into self-assembling peptide nano-fibre hydrogel. The cell-gel constructs were cultured for two weeks. β-glycerophosphate, dexamethasone and L-ascorbic acid were added into the culture medium for another two weeks. The cell-gel constructs were transplanted subcutaneously into nude mice. Plain gel constructs without human dental pulp cells were transplanted into the control sites. Four weeks after the surgeries, the transplants were harvested for antibody stainings and radiographic examinations. The percentages of radio-opaque areas were analysis with one sample t-test. Results: At all control sites, the gel constructs were completely resorbed with no new tissue formation. Radiographic examination showed that the specimens harvested from experiment sites contained mineralised nodules. The mean percentage for radio-opaque area was 63.6% (N=2, P= 0.014). Microscopic examination showed that the gel constructs have been transformed into whole pieces of vascularised tissue containing nodular structures. Antibody stainings showed the expression of type I collagen, osteopontin, osteocalcin and osteonectin in the nodules. Apparently they resembled developing bone. Conclusion: The results show that it is possible to produce small pieces, measured by millimeters, of mineralised tissue using human dental pulp cells and self-assembling peptide nano-fibre hydrogel. The feasibility of making larger pieces, measured by centimeters, is subjected to further investigation.
Persistent Identifierhttp://hdl.handle.net/10722/224474
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorChan, BKM-
dc.contributor.authorWong, RWK-
dc.contributor.authorRabie, ABM-
dc.date.accessioned2016-04-06T01:59:36Z-
dc.date.available2016-04-06T01:59:36Z-
dc.date.issued2010-
dc.identifier.citationThe 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010. In Journal of Dental Research, 2010, v. 89 n. Spec Iss C, p. Abstract no.97-
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/224474-
dc.description.abstractObjective: This study investigates the possibility to produce small pieces of mineralised tissue for future clinical use. Methods: Cells were isolated from human dental pulps. After culture and expansion the cells were encapsulated into self-assembling peptide nano-fibre hydrogel. The cell-gel constructs were cultured for two weeks. β-glycerophosphate, dexamethasone and L-ascorbic acid were added into the culture medium for another two weeks. The cell-gel constructs were transplanted subcutaneously into nude mice. Plain gel constructs without human dental pulp cells were transplanted into the control sites. Four weeks after the surgeries, the transplants were harvested for antibody stainings and radiographic examinations. The percentages of radio-opaque areas were analysis with one sample t-test. Results: At all control sites, the gel constructs were completely resorbed with no new tissue formation. Radiographic examination showed that the specimens harvested from experiment sites contained mineralised nodules. The mean percentage for radio-opaque area was 63.6% (N=2, P= 0.014). Microscopic examination showed that the gel constructs have been transformed into whole pieces of vascularised tissue containing nodular structures. Antibody stainings showed the expression of type I collagen, osteopontin, osteocalcin and osteonectin in the nodules. Apparently they resembled developing bone. Conclusion: The results show that it is possible to produce small pieces, measured by millimeters, of mineralised tissue using human dental pulp cells and self-assembling peptide nano-fibre hydrogel. The feasibility of making larger pieces, measured by centimeters, is subjected to further investigation.-
dc.languageeng-
dc.publisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/-
dc.relation.ispartofJournal of Dental Research-
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectBone-
dc.subjectBioengineering-
dc.subjectFormation-
dc.titleEngineering Mineralised Tissue Pieces using Human Dental Pulp Cells-
dc.typeConference_Paper-
dc.identifier.emailWong, RWK: fyoung@hkucc.hku.hk-
dc.identifier.emailRabie, ABM: rabie@hkusua.hku.hk-
dc.identifier.authorityWong, RWK=rp00038-
dc.identifier.authorityRabie, ABM=rp00029-
dc.identifier.hkuros183165-
dc.identifier.volume89-
dc.identifier.issueSpec Iss C-
dc.identifier.spageAbstract no.97-
dc.identifier.epageAbstract no.97-
dc.publisher.placeUnited States-

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