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Article: Application of the New Stereological Probes to the Study of the Melanosome in Cloudman S91 Melanoma Cells

TitleApplication of the New Stereological Probes to the Study of the Melanosome in Cloudman S91 Melanoma Cells
Authors
Issue Date1997
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/PCR
Citation
Pigment Cell Research, 1997, v. 10 n. 1-2, p. 77-84 How to Cite?
AbstractThe relationship between melanosome size and number and melanin content has been investigated in Cloudman S91 melanoma cells growing in vitro using both “model-based” and “design-based” stereological procedures. Cells were cultured for 4 days, harvested at daily intervals, and resin-embedded for light and electron microscopy; one aliquot of each sample of cells was assayed to determine its melanin content. By comparing their volume-weighted mean nuclear volume and their number-weighted mean nuclear volume, we have found that the nuclei of Cloudman melanoma cells form a fairly homogeneous population. The volume fraction and absolute volume of premelanosomes (Vvpm, cell and Vpm) and mature melanosomes (Vvmm, cell and Vmm) were all found to decrease progressively throughout the period of culture as did the number of premelanosomes (Npm) and mature melanosomes (Nmm). Whilst the volume-weighted mean volume of individual stage I and stage II premelanosomes, (vipm), remained fairly constant at about 10 nm3, the volume of individual stage III and IV mature melanosomes showed significant variation ranging between about 13 nm3 and 32 nm3. The melanin content of the cells decreased progressively over the 4 days of culture. There were, however, considerable variations in both the average melanin content per unit volume of mature melanosomes, in the range 170–600 fg/μm3, and in the melanin content per individual mature melanosome, in the range 3–12 fg. Our findings show that stereological techniques can provide unbiased and sensitive tools for the study of the morphological basis of melanogenesis; their value will become even more evident when they are combined with techniques for the localization of melanogenic enzymes and their substrates.
Persistent Identifierhttp://hdl.handle.net/10722/224373
ISSN
2007 Impact Factor: 4.288

 

DC FieldValueLanguage
dc.contributor.authorFang, D-
dc.contributor.authorDockery, P-
dc.contributor.authorWeatherhead, B-
dc.date.accessioned2016-04-01T03:36:00Z-
dc.date.available2016-04-01T03:36:00Z-
dc.date.issued1997-
dc.identifier.citationPigment Cell Research, 1997, v. 10 n. 1-2, p. 77-84-
dc.identifier.issn0893-5785-
dc.identifier.urihttp://hdl.handle.net/10722/224373-
dc.description.abstractThe relationship between melanosome size and number and melanin content has been investigated in Cloudman S91 melanoma cells growing in vitro using both “model-based” and “design-based” stereological procedures. Cells were cultured for 4 days, harvested at daily intervals, and resin-embedded for light and electron microscopy; one aliquot of each sample of cells was assayed to determine its melanin content. By comparing their volume-weighted mean nuclear volume and their number-weighted mean nuclear volume, we have found that the nuclei of Cloudman melanoma cells form a fairly homogeneous population. The volume fraction and absolute volume of premelanosomes (Vvpm, cell and Vpm) and mature melanosomes (Vvmm, cell and Vmm) were all found to decrease progressively throughout the period of culture as did the number of premelanosomes (Npm) and mature melanosomes (Nmm). Whilst the volume-weighted mean volume of individual stage I and stage II premelanosomes, (vipm), remained fairly constant at about 10 nm3, the volume of individual stage III and IV mature melanosomes showed significant variation ranging between about 13 nm3 and 32 nm3. The melanin content of the cells decreased progressively over the 4 days of culture. There were, however, considerable variations in both the average melanin content per unit volume of mature melanosomes, in the range 170–600 fg/μm3, and in the melanin content per individual mature melanosome, in the range 3–12 fg. Our findings show that stereological techniques can provide unbiased and sensitive tools for the study of the morphological basis of melanogenesis; their value will become even more evident when they are combined with techniques for the localization of melanogenic enzymes and their substrates.-
dc.languageeng-
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/PCR-
dc.relation.ispartofPigment Cell Research-
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.subject.meshCell Size-
dc.subject.meshMelanins - analysis-
dc.subject.meshMelanocytes - chemistry - ultrastructure-
dc.subject.meshMelanoma, Experimental - pathology-
dc.subject.meshTumor Cells, Cultured-
dc.titleApplication of the New Stereological Probes to the Study of the Melanosome in Cloudman S91 Melanoma Cells-
dc.typeArticle-
dc.identifier.emailWeatherhead, B: weatherb@hkucc.hku.hk-
dc.identifier.doi10.1111/j.1600-0749.1997.tb00471.x-
dc.identifier.pmid9170168-
dc.identifier.hkuros23039-
dc.identifier.volume10-
dc.identifier.issue1-2-
dc.identifier.spage77-
dc.identifier.epage84-
dc.publisher.placeDenmark-

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