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Conference Paper: Pseudomonas aeruginosa lipopolysaccharide enhances Candida albicans hypha specific gene expression

TitlePseudomonas aeruginosa lipopolysaccharide enhances Candida albicans hypha specific gene expression
Authors
KeywordsBiofilm
Microbiology
Molecular biology
Gene expression
Fungi
Issue Date2010
PublisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/
Citation
The 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010. In Journal of Dental Research, 2010, v. 89 n. Spec Iss C, p. Abstract no. 92 How to Cite?
AbstractIntroduction: Demystification of cellular interactions in mixed biofilms has a major impact on therapeutics. Objective: Evaluation of the effect of bacterial lipopolysaccharide (LPS) on Candida albicans biofilm formation and its hyphal development. Method: The effect Pseudomonas aeruginosa LPS on C. albicans SC 5314 was studied using a standard biofilm assay. Biofilm formation of LPS treated C. albicans on polystyrene surfaces was quantified at 90min, 24h and 48h by a XTT reduction assay. Biofilms were qualitatively and quantitatively analyzed using Confocal Laser Scanning Microscopy (CLSM) and Scanning Electron Microscopy (SEM). The effect of LPS on hypha specific genes (HSGs) of C. albicans was evaluated with quantitative Real time PCR. Farnesol, a natural pesticide, was also evaluated. Differential expression of HSGs; ECE1, HWP1, HYR1, RBT1, RBT4, ALS3 and ALS8 were assessed at 24h biofilms. Results: Adhesion of C. albicans (90 min) was significantly enhanced by P. aeruginosa LPS (P<0.05). CLSM and SEM further confirmed these data. All HSGs tested were significantly upregulated when treated with LPS and was significantly downregulated when treated with farnesol (p<0.05). Conclusion: P. aeruginosa LPS significantly enhances in vitro C. albicans hyphal development though it does not appear to affect the Candida biofilm metabolic activity.
Persistent Identifierhttp://hdl.handle.net/10722/224280
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorBandara, HMHN-
dc.contributor.authorCheung, BPK-
dc.contributor.authorWatt, RM-
dc.contributor.authorJin, LJ-
dc.contributor.authorSamaranayake, LP-
dc.date.accessioned2016-03-31T03:11:51Z-
dc.date.available2016-03-31T03:11:51Z-
dc.date.issued2010-
dc.identifier.citationThe 24th IADR-SEA Division Annual Scientific Meeting, Taipei, Taiwan, 19-21 September 2010. In Journal of Dental Research, 2010, v. 89 n. Spec Iss C, p. Abstract no. 92-
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/224280-
dc.description.abstractIntroduction: Demystification of cellular interactions in mixed biofilms has a major impact on therapeutics. Objective: Evaluation of the effect of bacterial lipopolysaccharide (LPS) on Candida albicans biofilm formation and its hyphal development. Method: The effect Pseudomonas aeruginosa LPS on C. albicans SC 5314 was studied using a standard biofilm assay. Biofilm formation of LPS treated C. albicans on polystyrene surfaces was quantified at 90min, 24h and 48h by a XTT reduction assay. Biofilms were qualitatively and quantitatively analyzed using Confocal Laser Scanning Microscopy (CLSM) and Scanning Electron Microscopy (SEM). The effect of LPS on hypha specific genes (HSGs) of C. albicans was evaluated with quantitative Real time PCR. Farnesol, a natural pesticide, was also evaluated. Differential expression of HSGs; ECE1, HWP1, HYR1, RBT1, RBT4, ALS3 and ALS8 were assessed at 24h biofilms. Results: Adhesion of C. albicans (90 min) was significantly enhanced by P. aeruginosa LPS (P<0.05). CLSM and SEM further confirmed these data. All HSGs tested were significantly upregulated when treated with LPS and was significantly downregulated when treated with farnesol (p<0.05). Conclusion: P. aeruginosa LPS significantly enhances in vitro C. albicans hyphal development though it does not appear to affect the Candida biofilm metabolic activity.-
dc.languageeng-
dc.publisherSage Publications, Inc. The Journal's web site is located at http://jdr.sagepub.com/-
dc.relation.ispartofJournal of Dental Research-
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectBiofilm-
dc.subjectMicrobiology-
dc.subjectMolecular biology-
dc.subjectGene expression-
dc.subjectFungi-
dc.titlePseudomonas aeruginosa lipopolysaccharide enhances Candida albicans hypha specific gene expression-
dc.typeConference_Paper-
dc.identifier.emailBandara, HMHN: nihal@hkusua.hku.hk-
dc.identifier.emailCheung, BPK: bpkcheun@HKUCC.hku.hk-
dc.identifier.emailWatt, RM: rmwatt@hkucc.hku.hk-
dc.identifier.emailJin, LJ: ljjin@hkusua.hku.hk-
dc.identifier.emailSamaranayake, LP: lakshman@hku.hk-
dc.identifier.authorityWatt, RM=rp00043-
dc.identifier.authorityJin, LJ=rp00028-
dc.identifier.authoritySamaranayake, LP=rp00023-
dc.identifier.hkuros181597-
dc.identifier.volume89-
dc.identifier.issueSpec Iss C-
dc.identifier.spageAbstract no. 92-
dc.identifier.epageAbstract no. 92-
dc.publisher.placeUnited States-

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