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Article: Production of matrix metalloproteinases in specific subpopulations of human-patient breast cancer invading in three dimensional culture system

TitleProduction of matrix metalloproteinases in specific subpopulations of human-patient breast cancer invading in three dimensional culture system
Authors
Issue Date2004
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 2004, v. 76 n. 7, p. 743-752 How to Cite?
AbstractThis article aims at investigating the effect of production of matrix metalloproteinases (MMP) in human breast cancer tissues by means of three dimensional culture system. Thirty-nine tumour samples were taken from breast cancer patients. The tumour blocks were cultured on sponge gel using the three dimensional culture system. Breast cancer cells began shedding into the culture medium after 24 hours of culture. The cells were stained with trypan blue dye to assess viability on days 2, 4, 6 and 8. The culture medium was collected at these time points and tested for matrix metalloproteinases (MMP) 1,2,3 and 9 activities. There was a progressive increase in migration of cancer cells into the gel and culture medium from day 2 to day 8 and the interval difference was statistically significant (F ratio = 4.06; p = 0.008). The levels of all the MMPs tested were also significantly raised (P<0.05 for all the MMPs tested). When the levels of MMPs were correlated with the metabolic activities in the gel, medium and tumour block, cells in block show no correlation whereas cells in gel correlated significantly with MMP-1 and MMP-3. Cancer cells in the culture medium correlated with MMP-9. In conclusion, there is a progressive migration of cancer cells outside the tumour block. The migration into the gel and culture medium is associated with progressive and differential production of MMPs. It is likely that the three dimensional culture model assists in the selection of different subpopulations of cancer cells with different invasion properties as exemplified by the differential production of MMP.
Persistent Identifierhttp://hdl.handle.net/10722/224128
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056

 

DC FieldValueLanguage
dc.contributor.authorLoo, TY-
dc.contributor.authorCheung, MNB-
dc.contributor.authorChow, LWC-
dc.date.accessioned2016-03-24T04:38:55Z-
dc.date.available2016-03-24T04:38:55Z-
dc.date.issued2004-
dc.identifier.citationLife Sciences, 2004, v. 76 n. 7, p. 743-752-
dc.identifier.issn0024-3205-
dc.identifier.urihttp://hdl.handle.net/10722/224128-
dc.description.abstractThis article aims at investigating the effect of production of matrix metalloproteinases (MMP) in human breast cancer tissues by means of three dimensional culture system. Thirty-nine tumour samples were taken from breast cancer patients. The tumour blocks were cultured on sponge gel using the three dimensional culture system. Breast cancer cells began shedding into the culture medium after 24 hours of culture. The cells were stained with trypan blue dye to assess viability on days 2, 4, 6 and 8. The culture medium was collected at these time points and tested for matrix metalloproteinases (MMP) 1,2,3 and 9 activities. There was a progressive increase in migration of cancer cells into the gel and culture medium from day 2 to day 8 and the interval difference was statistically significant (F ratio = 4.06; p = 0.008). The levels of all the MMPs tested were also significantly raised (P<0.05 for all the MMPs tested). When the levels of MMPs were correlated with the metabolic activities in the gel, medium and tumour block, cells in block show no correlation whereas cells in gel correlated significantly with MMP-1 and MMP-3. Cancer cells in the culture medium correlated with MMP-9. In conclusion, there is a progressive migration of cancer cells outside the tumour block. The migration into the gel and culture medium is associated with progressive and differential production of MMPs. It is likely that the three dimensional culture model assists in the selection of different subpopulations of cancer cells with different invasion properties as exemplified by the differential production of MMP.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie-
dc.relation.ispartofLife Sciences-
dc.rightsPosting accepted manuscript (postprint): © <year>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.subject.meshAdenosine Triphosphate - analysis - metabolism-
dc.subject.meshBreast Neoplasms - enzymology - pathology-
dc.subject.meshCarcinoma, Ductal, Breast - enzymology - pathology-
dc.subject.meshMatrix Metalloproteinases - biosynthesis-
dc.subject.meshTissue Inhibitor of Metalloproteinase-1 - biosynthesis-
dc.titleProduction of matrix metalloproteinases in specific subpopulations of human-patient breast cancer invading in three dimensional culture system-
dc.typeArticle-
dc.identifier.emailLoo, TY: tyloo@hkucc.hku.hk-
dc.identifier.emailChow, LWC: lwcchow@hkucc.hku.hk-
dc.identifier.doi10.1016/j.lfs.2004.06.027-
dc.identifier.pmid15581906-
dc.identifier.scopuseid_2-s2.0-9644302845-
dc.identifier.hkuros96741-
dc.identifier.volume76-
dc.identifier.issue7-
dc.identifier.spage743-
dc.identifier.epage752-
dc.publisher.placeUnited States-

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