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Article: E-cadherin promoter methylation can regulate its expression in invasive ductal breast cancer tissue in Chinese woman

TitleE-cadherin promoter methylation can regulate its expression in invasive ductal breast cancer tissue in Chinese woman
Authors
Issue Date2002
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 2002, v. 71 n. 12, p. 1397-1404 How to Cite?
AbstractPromoter methylation is an important mechanism of regulating E-cadherin expression. Methylation-specific PCR (MSP) assay was done to evaluate the promoter methylation status of E-cadherin gene in primary tumor samples from 23 cases of Chinese women with invasive ductal breast cancers. Western blotting assay was employed for E-cadherin and β-actin expressions. Positive MSP results occurred in 26.1% (6/23) of primary tumor samples and none of four normal skin samples. These molecular events tended to occur in breast cancers associated with poor prognosis. Whereas the mean ratio of CDH1/β-actin for six MSP-positive cases was 0.0290 ± 0.0355, the mean ratio for 17 MSP-negative cases was 0.4726 ± 0.5049 (P = 0.046). In conclusion, aberrant E-cadherin methylation preferentially occurs in invasive ductal breast cancer associated with poor prognosis and is one of the mechanisms of E-cadherin expression silence in breast cancers from Chinese women.
Persistent Identifierhttp://hdl.handle.net/10722/224121
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056

 

DC FieldValueLanguage
dc.contributor.authorHu, XC-
dc.contributor.authorLoo, WT-
dc.contributor.authorChow, LWC-
dc.date.accessioned2016-03-24T03:48:29Z-
dc.date.available2016-03-24T03:48:29Z-
dc.date.issued2002-
dc.identifier.citationLife Sciences, 2002, v. 71 n. 12, p. 1397-1404-
dc.identifier.issn0024-3205-
dc.identifier.urihttp://hdl.handle.net/10722/224121-
dc.description.abstractPromoter methylation is an important mechanism of regulating E-cadherin expression. Methylation-specific PCR (MSP) assay was done to evaluate the promoter methylation status of E-cadherin gene in primary tumor samples from 23 cases of Chinese women with invasive ductal breast cancers. Western blotting assay was employed for E-cadherin and β-actin expressions. Positive MSP results occurred in 26.1% (6/23) of primary tumor samples and none of four normal skin samples. These molecular events tended to occur in breast cancers associated with poor prognosis. Whereas the mean ratio of CDH1/β-actin for six MSP-positive cases was 0.0290 ± 0.0355, the mean ratio for 17 MSP-negative cases was 0.4726 ± 0.5049 (P = 0.046). In conclusion, aberrant E-cadherin methylation preferentially occurs in invasive ductal breast cancer associated with poor prognosis and is one of the mechanisms of E-cadherin expression silence in breast cancers from Chinese women.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie-
dc.relation.ispartofLife Sciences-
dc.subject.meshBreast Neoplasms - genetics-
dc.subject.meshCadherins - genetics-
dc.subject.meshCarcinoma, Ductal, Breast - genetics-
dc.subject.meshGene Expression Regulation, Neoplastic - genetics-
dc.subject.meshPromoter Regions, Genetic - genetics-
dc.titleE-cadherin promoter methylation can regulate its expression in invasive ductal breast cancer tissue in Chinese woman-
dc.typeArticle-
dc.identifier.emailChow, LWC: lwcchow@hkucc.hku.hk-
dc.identifier.doi10.1016/S0024-3205(02)01843-X-
dc.identifier.pmid12127160-
dc.identifier.scopuseid_2-s2.0-0037047459-
dc.identifier.hkuros73259-
dc.identifier.volume71-
dc.identifier.issue12-
dc.identifier.spage1397-
dc.identifier.epage1404-
dc.publisher.placeUnited States-

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