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Article: α-Adrenoceptor subtypes of dog saphenous vein: Another unusual property

Titleα-Adrenoceptor subtypes of dog saphenous vein: Another unusual property
Authors
Issue Date1998
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 1998, v. 62 n. 17-18, p. 1455-1459 How to Cite?
AbstractThe functional relationship between vascular smooth muscle α1- and α2-adrenoceptor (AR) subtypes was investigated by simultaneous measurement of contractile and fluorescence ratio in fura-2 loaded rings of dog saphenous vein (DSV). Prazosin, as well as rauwolscine, at 0.1 μM, substantially antagonized contractions and associated cytosolic [Ca2+] rises induced by UK 14304, while rauwolscine, as well as prazosin, antagonized similar effects of phenylephrine (PE). These antagonisms were characterized by a parallel rightward shift of the concentration-response curves. In the absence of extracellular Ca2+, PE as well as UK 14304 caused simultaneous transient elevation of contractile force and cytosolic [Ca2+], although the UK 14304 responses were smaller than PE responses. We propose that DSV smooth muscle cells possess interacting α1- and α2-ARs which have overlapping functional domain sensitive to the agonists and antagonists of either α-AR subtype. Both α-AR subtypes appear to utilize similar signaling mechanisms via Ca2+ release from the same intracellular stores and Ca2+ entry across the plasma membrane.
Persistent Identifierhttp://hdl.handle.net/10722/224111
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056

 

DC FieldValueLanguage
dc.contributor.authorMo, FM-
dc.contributor.authorKwan, CY-
dc.date.accessioned2016-03-24T01:55:30Z-
dc.date.available2016-03-24T01:55:30Z-
dc.date.issued1998-
dc.identifier.citationLife Sciences, 1998, v. 62 n. 17-18, p. 1455-1459-
dc.identifier.issn0024-3205-
dc.identifier.urihttp://hdl.handle.net/10722/224111-
dc.description.abstractThe functional relationship between vascular smooth muscle α1- and α2-adrenoceptor (AR) subtypes was investigated by simultaneous measurement of contractile and fluorescence ratio in fura-2 loaded rings of dog saphenous vein (DSV). Prazosin, as well as rauwolscine, at 0.1 μM, substantially antagonized contractions and associated cytosolic [Ca2+] rises induced by UK 14304, while rauwolscine, as well as prazosin, antagonized similar effects of phenylephrine (PE). These antagonisms were characterized by a parallel rightward shift of the concentration-response curves. In the absence of extracellular Ca2+, PE as well as UK 14304 caused simultaneous transient elevation of contractile force and cytosolic [Ca2+], although the UK 14304 responses were smaller than PE responses. We propose that DSV smooth muscle cells possess interacting α1- and α2-ARs which have overlapping functional domain sensitive to the agonists and antagonists of either α-AR subtype. Both α-AR subtypes appear to utilize similar signaling mechanisms via Ca2+ release from the same intracellular stores and Ca2+ entry across the plasma membrane.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie-
dc.relation.ispartofLife Sciences-
dc.subject.meshAdrenergic alpha-Agonists - pharmacology-
dc.subject.meshMuscle Contraction - drug effects - physiology-
dc.subject.meshMuscle, Smooth, Vascular - drug effects - physiology - ultrastructure-
dc.subject.meshReceptors, Adrenergic, alpha - classification - drug effects - physiology-
dc.subject.meshSaphenous Vein - drug effects - physiology - ultrastructure-
dc.titleα-Adrenoceptor subtypes of dog saphenous vein: Another unusual property-
dc.typeArticle-
dc.identifier.emailKwan, CY: cykwan@hkucc.hku.hk-
dc.identifier.doi10.1016/S0024-3205(98)00089-7-
dc.identifier.pmid9585118-
dc.identifier.scopuseid_2-s2.0-0032571285-
dc.identifier.hkuros38365-
dc.identifier.volume62-
dc.identifier.issue17-18-
dc.identifier.spage1455-
dc.identifier.epage1459-
dc.publisher.placeUnited States-

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