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Article: Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling

TitleUsp12 stabilizes the T-cell receptor complex at the cell surface during signaling
Authors
Issue Date2016
Citation
Proceedings of the National Academy of Sciences, 2016, v. 113 n. 6, p. E705-E714 How to Cite?
AbstractPosttranslational modifications are central to the spatial and temporal regulation of protein function. Among others, phosphorylation and ubiquitylation are known to regulate proximal T-cell receptor (TCR) signaling. Here we used a systematic and unbiased approach to uncover deubiquitylating enzymes (DUBs) that participate during TCR signaling in primary mouse T lymphocytes. Using a C-terminally modified vinyl methyl ester variant of ubiquitin (HA-Ub-VME), we captured DUBs that are differentially recruited to the cytosol on TCR activation. We identified ubiquitin-specific peptidase (Usp) 12 and Usp46, which had not been previously described in this pathway. Stimulation with anti-CD3 resulted in phosphorylation and time-dependent translocation of Usp12 from the nucleus to the cytosol. Usp12(-/-) Jurkat cells displayed defective NFκB, NFAT, and MAPK activities owing to attenuated surface expression of TCR, which were rescued on reconstitution of wild type Usp12. Proximity-based labeling with BirA-Usp12 revealed several TCR adaptor proteins acting as interactors in stimulated cells, of which LAT and Trat1 displayed reduced expression in Usp12(-/-) cells. We demonstrate that Usp12 deubiquitylates and prevents lysosomal degradation of LAT and Trat1 to maintain the proximal TCR complex for the duration of signaling. Our approach benefits from the use of activity-based probes in primary cells without any previous genome modification, and underscores the importance of ubiquitin-mediated regulation to refine signaling cascades
Persistent Identifierhttp://hdl.handle.net/10722/223972
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorJahan, AS-
dc.contributor.authorLestra, M-
dc.contributor.authorSwee, LK-
dc.contributor.authorFan, Y-
dc.contributor.authorLamers, MM-
dc.contributor.authorTafesse, FG-
dc.contributor.authorTheile, CS-
dc.contributor.authorSpooner, E-
dc.contributor.authorBruzzone, R-
dc.contributor.authorPloegh, HL-
dc.contributor.authorSanyal, S-
dc.date.accessioned2016-03-18T02:33:04Z-
dc.date.available2016-03-18T02:33:04Z-
dc.date.issued2016-
dc.identifier.citationProceedings of the National Academy of Sciences, 2016, v. 113 n. 6, p. E705-E714-
dc.identifier.urihttp://hdl.handle.net/10722/223972-
dc.description.abstractPosttranslational modifications are central to the spatial and temporal regulation of protein function. Among others, phosphorylation and ubiquitylation are known to regulate proximal T-cell receptor (TCR) signaling. Here we used a systematic and unbiased approach to uncover deubiquitylating enzymes (DUBs) that participate during TCR signaling in primary mouse T lymphocytes. Using a C-terminally modified vinyl methyl ester variant of ubiquitin (HA-Ub-VME), we captured DUBs that are differentially recruited to the cytosol on TCR activation. We identified ubiquitin-specific peptidase (Usp) 12 and Usp46, which had not been previously described in this pathway. Stimulation with anti-CD3 resulted in phosphorylation and time-dependent translocation of Usp12 from the nucleus to the cytosol. Usp12(-/-) Jurkat cells displayed defective NFκB, NFAT, and MAPK activities owing to attenuated surface expression of TCR, which were rescued on reconstitution of wild type Usp12. Proximity-based labeling with BirA-Usp12 revealed several TCR adaptor proteins acting as interactors in stimulated cells, of which LAT and Trat1 displayed reduced expression in Usp12(-/-) cells. We demonstrate that Usp12 deubiquitylates and prevents lysosomal degradation of LAT and Trat1 to maintain the proximal TCR complex for the duration of signaling. Our approach benefits from the use of activity-based probes in primary cells without any previous genome modification, and underscores the importance of ubiquitin-mediated regulation to refine signaling cascades-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences-
dc.titleUsp12 stabilizes the T-cell receptor complex at the cell surface during signaling-
dc.typeArticle-
dc.identifier.emailFan, Y: ivonnayf@hku.hk-
dc.identifier.emailBruzzone, R: bruzzone@hkucc.hku.hk-
dc.identifier.emailSanyal, S: sanyal@hku.hk-
dc.identifier.authorityBruzzone, R=rp01442-
dc.identifier.authoritySanyal, S=rp01794-
dc.identifier.doi10.1073/pnas.1521763113-
dc.identifier.pmid26811477-
dc.identifier.pmcidPMC4760780-
dc.identifier.hkuros257236-
dc.identifier.volume113-
dc.identifier.issue6-
dc.identifier.spageE705-
dc.identifier.epageE714-

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