File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Analysis of 5-methoxytryptamine at the femtomole level in the rat and quail brain by gas chromatography-electron-capture negative-ion chemical ionization mass spectrometry

TitleAnalysis of 5-methoxytryptamine at the femtomole level in the rat and quail brain by gas chromatography-electron-capture negative-ion chemical ionization mass spectrometry
Authors
Issue Date1996
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jchromb
Citation
Journal of Chromatography B, Biomedical sciences and applications, 1996, v. 682 n. 2, p. 185-194 How to Cite?
AbstractA sensitive method for the measurement of endogenous 5-methoxytryptamine in brain tissue has been developed using capillary column gas chromatography-electron-capture negative-ion chemical ionization mass spectrometry. 5-Methoxytryptamine was first converted to N-[2H3]acetyl-5-methoxytryptamine by reaction with hexa-deuterated acetic anhydride, followed by reaction with pentafluoropropionic anhydride to yield the highly electron-capturing 3,3′-spirocyclic pentafluoropropionyl indolenine derivative. Quantitative analysis was carried out by selected-ion monitoring of the [M-HF] and [M-HF-DF] ion intensity of the 3,3′-spirocyclic pentafluoropropionyl indolenine derivative, using 5-methoxy-[α,α,β,β-2H4]tryptamine as the internal standard. The presence of 5-methoxytryptamine in the brain tissue was demonstrated. In the absence of a monoamine oxidase inhibitor, the mean±S.D. levels of 5-methoxytryptamine in the rat and quail whole brain were found to be 30±6 and 347±52 pg/g, respectively. The possible physiological functions of 5-methoxytryptamine as a neuromodulator and/or neurotransmitter have to be considered.
Persistent Identifierhttp://hdl.handle.net/10722/223743
ISSN
2001 Impact Factor: 1.911

 

DC FieldValueLanguage
dc.contributor.authorTsang, CW-
dc.contributor.authorChan, CL-
dc.contributor.authorLi, P-
dc.contributor.authorPang, SF-
dc.date.accessioned2016-03-14T02:54:07Z-
dc.date.available2016-03-14T02:54:07Z-
dc.date.issued1996-
dc.identifier.citationJournal of Chromatography B, Biomedical sciences and applications, 1996, v. 682 n. 2, p. 185-194-
dc.identifier.issn0378-4347-
dc.identifier.urihttp://hdl.handle.net/10722/223743-
dc.description.abstractA sensitive method for the measurement of endogenous 5-methoxytryptamine in brain tissue has been developed using capillary column gas chromatography-electron-capture negative-ion chemical ionization mass spectrometry. 5-Methoxytryptamine was first converted to N-[2H3]acetyl-5-methoxytryptamine by reaction with hexa-deuterated acetic anhydride, followed by reaction with pentafluoropropionic anhydride to yield the highly electron-capturing 3,3′-spirocyclic pentafluoropropionyl indolenine derivative. Quantitative analysis was carried out by selected-ion monitoring of the [M-HF] and [M-HF-DF] ion intensity of the 3,3′-spirocyclic pentafluoropropionyl indolenine derivative, using 5-methoxy-[α,α,β,β-2H4]tryptamine as the internal standard. The presence of 5-methoxytryptamine in the brain tissue was demonstrated. In the absence of a monoamine oxidase inhibitor, the mean±S.D. levels of 5-methoxytryptamine in the rat and quail whole brain were found to be 30±6 and 347±52 pg/g, respectively. The possible physiological functions of 5-methoxytryptamine as a neuromodulator and/or neurotransmitter have to be considered.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jchromb-
dc.relation.ispartofJournal of Chromatography B, Biomedical sciences and applications-
dc.rightsPosting accepted manuscript (postprint): © <year>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.subject.mesh5-Methoxytryptamine - analysis-
dc.subject.meshBrain Chemistry-
dc.subject.meshGas Chromatography-Mass Spectrometry - methods-
dc.subject.meshRats, Sprague-Dawley-
dc.subject.meshReproducibility of Results-
dc.titleAnalysis of 5-methoxytryptamine at the femtomole level in the rat and quail brain by gas chromatography-electron-capture negative-ion chemical ionization mass spectrometry-
dc.typeArticle-
dc.identifier.emailPang, SF: hrmypsf@hkucc.hku.hk-
dc.identifier.doi10.1016/0378-4347(96)00099-0-
dc.identifier.pmid8844409-
dc.identifier.hkuros25270-
dc.identifier.volume682-
dc.identifier.issue2-
dc.identifier.spage185-
dc.identifier.epage194-
dc.publisher.placeNetherlands-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats