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Article: An evaluation of the fungal 'morphotype' concept based on ribosomal DNA sequences

TitleAn evaluation of the fungal 'morphotype' concept based on ribosomal DNA sequences
Authors
KeywordsFungal endophytes
Fungal morphospecies
Mycelia sterilia
rDNA
Issue Date2003
PublisherFungal Diversity Press. The Journal's web site is located at http://www.fungaldiversity.org/fdp/jumble.php
Citation
Fungal Diversity, 2003, v. 12, p. 53-66 How to Cite?
AbstractIn studies of fungal endophyte communities, mycelia sterilia are commonly isolated from plant substrates and grouped into morphotypes on the basis of cultural characteristics. In Polygonum multiflorum one hundred and sixty-nine strains of mycelia sterilia were isolated and grouped into 27 morphotypes. Six randomly selected morphotypes, each with 2-3 representatives, were subsequently subjected to ribosomal DNA sequence analysis. Nucleotide sequence similarities of the rDNA internal transcribed spacer (ITS) regions and the 5.8S gene were compared using UPGMA cluster analysis. Comparison of nucleotide sequences revealed high levels of similarity (ca. 91.63-99.53%) among strains within morphotypes. ITS and 5.8S sequences of species within various genera from GenBank were obtained to estimate levels of nucleotide similarity within and between well-established genera and species. This study verifies on the basis of ribosomal DNA sequence analysis the validity of these 'morphotypes' as taxonomic groups. A dendogram, illustrating relatedness of the morphotypes and reference taxa from GenBank is also presented.
Persistent Identifierhttp://hdl.handle.net/10722/223069
ISSN
2015 Impact Factor: 6.991
2015 SCImago Journal Rankings: 3.027

 

DC FieldValueLanguage
dc.contributor.authorLacap, DC-
dc.contributor.authorHyde, KD-
dc.contributor.authorLiew, ECY-
dc.date.accessioned2016-02-18T03:35:25Z-
dc.date.available2016-02-18T03:35:25Z-
dc.date.issued2003-
dc.identifier.citationFungal Diversity, 2003, v. 12, p. 53-66-
dc.identifier.issn1560-2745-
dc.identifier.urihttp://hdl.handle.net/10722/223069-
dc.description.abstractIn studies of fungal endophyte communities, mycelia sterilia are commonly isolated from plant substrates and grouped into morphotypes on the basis of cultural characteristics. In Polygonum multiflorum one hundred and sixty-nine strains of mycelia sterilia were isolated and grouped into 27 morphotypes. Six randomly selected morphotypes, each with 2-3 representatives, were subsequently subjected to ribosomal DNA sequence analysis. Nucleotide sequence similarities of the rDNA internal transcribed spacer (ITS) regions and the 5.8S gene were compared using UPGMA cluster analysis. Comparison of nucleotide sequences revealed high levels of similarity (ca. 91.63-99.53%) among strains within morphotypes. ITS and 5.8S sequences of species within various genera from GenBank were obtained to estimate levels of nucleotide similarity within and between well-established genera and species. This study verifies on the basis of ribosomal DNA sequence analysis the validity of these 'morphotypes' as taxonomic groups. A dendogram, illustrating relatedness of the morphotypes and reference taxa from GenBank is also presented.-
dc.languageeng-
dc.publisherFungal Diversity Press. The Journal's web site is located at http://www.fungaldiversity.org/fdp/jumble.php-
dc.relation.ispartofFungal Diversity-
dc.subjectFungal endophytes-
dc.subjectFungal morphospecies-
dc.subjectMycelia sterilia-
dc.subjectrDNA-
dc.titleAn evaluation of the fungal 'morphotype' concept based on ribosomal DNA sequences-
dc.typeArticle-
dc.identifier.emailHyde, KD: kdhyde@hkucc.hku.hk-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.scopuseid_2-s2.0-0346021440-
dc.identifier.hkuros81652-
dc.identifier.volume12-
dc.identifier.spage53-
dc.identifier.epage66-
dc.publisher.placeHong Kong-

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