granted patent: Method for extracting mesenchymal stem cell from human or animal embryo and for extracting the secretion product thereof

TitleMethod for extracting mesenchymal stem cell from human or animal embryo and for extracting the secretion product thereof
Granted PatentUS 20110217385
Granted Date2011-09-08
Priority Date2008-10-10 PCT/CN2008072648
2009-09-18 PCT/CN2009074031
Inventors
Issue Date2011
Citation
US Patent US 20110217385. Washington, DC: US Patent and Trademark Office (USPTO), 2011 How to Cite?
AbstractThe present invention relates to a method of extracting mesenchymal stem cells from human or animal embryo, comprising: A. taking the sample based on human or animal embryo, centrifuging the sample; B. digesting the sample with enzyme digestion solution, and making single cell suspension, wherein said enzyme digestion solution comprises trysin-EDTA and collagenase IV; C. adding mesenchymal stem cell culture medium A to the isolated single cells, inoculating and culturing the cells; D. treating the cells with adherence purification, and obtaining mesenchymal stem cells by magnetic sorting. The present invention also relates to a method of extracting the secretion product from mesenchymal stem cells, the use of the secretion product in the aspects of cosmetic and healthcare, and an epidermal cell repairing lotion made using the secretion product. In the process according to the present invention of extracting mesenchymal stem cells from human or animal embryo, the mixture of trypsin-EDTA and collagenase IV is used as the enzyme digestion solution, enabling to obtain the maximal number of single cells, while reducing cell damage to the lowest level.
Persistent Identifierhttp://hdl.handle.net/10722/221643
References

 

DC FieldValueLanguage
dc.date.accessioned2015-11-30T04:05:25Z-
dc.date.available2015-11-30T04:05:25Z-
dc.date.issued2011-
dc.identifier.citationUS Patent US 20110217385. Washington, DC: US Patent and Trademark Office (USPTO), 2011-
dc.identifier.urihttp://hdl.handle.net/10722/221643-
dc.description.abstractThe present invention relates to a method of extracting mesenchymal stem cells from human or animal embryo, comprising: A. taking the sample based on human or animal embryo, centrifuging the sample; B. digesting the sample with enzyme digestion solution, and making single cell suspension, wherein said enzyme digestion solution comprises trysin-EDTA and collagenase IV; C. adding mesenchymal stem cell culture medium A to the isolated single cells, inoculating and culturing the cells; D. treating the cells with adherence purification, and obtaining mesenchymal stem cells by magnetic sorting. The present invention also relates to a method of extracting the secretion product from mesenchymal stem cells, the use of the secretion product in the aspects of cosmetic and healthcare, and an epidermal cell repairing lotion made using the secretion product. In the process according to the present invention of extracting mesenchymal stem cells from human or animal embryo, the mixture of trypsin-EDTA and collagenase IV is used as the enzyme digestion solution, enabling to obtain the maximal number of single cells, while reducing cell damage to the lowest level.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License for public patent documents-
dc.titleMethod for extracting mesenchymal stem cell from human or animal embryo and for extracting the secretion product thereof-
dc.typePatent-
dc.identifier.authorityLIAN QIZHOU=rp00267-
dc.description.naturepublished_or_final_version-
dc.contributor.inventorLIAN QIZHOU-
dc.contributor.inventorXIA JIANCHUAN-
patents.identifier.applicationUS 12/678745-
patents.identifier.grantedUS 20110217385-
patents.description.assigneeTEAM YOUN BIOMEDICAL TECHNOLOGY CO LTD [CN] -
patents.description.countryUnited States of America -
patents.date.granted2011-09-08-
dc.relation.referencesUS 2009136459 (A1) 2009-05-28-
patents.date.application2009-09-18-
patents.date.priority2008-10-10 PCT/CN2008072648-
patents.date.priority2009-09-18 PCT/CN2009074031-
patents.description.ccus-
patents.relation.familyAU 2009301517 (A1) 2010-04-15-
patents.relation.familyCA 2740092 (A1) 2010-04-15-
patents.relation.familyCN 101802177 (A) 2010-08-11-
patents.relation.familyEP 2368974 (A1) 2011-09-28-
patents.relation.familyJP 2010538681 (A) 2010-12-16-
patents.relation.familyKR 20100065338 (A) 2010-06-16-
patents.relation.familyWO 2010040262 (A1) 2010-04-15-
patents.relation.familyWO 2010040302 (A1) 2010-04-15-
patents.description.kindA1-
patents.typePatent_granted-

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