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postgraduate thesis: Development of recombinant adeno-associated virus-mediated delivery adiponectin in mice

TitleDevelopment of recombinant adeno-associated virus-mediated delivery adiponectin in mice
Authors
Issue Date2015
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
Citation
Chau, C. [周志強]. (2015). Development of recombinant adeno-associated virus-mediated delivery adiponectin in mice. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5617458
AbstractObesity is a global health problem experienced with various kinds of metabolic syndromes such as insulin resistance, hyperlipidemia and hypoadiponectinemia. To ameliorate obesity related problems, attention has been drawn to explore the role of adiponectin in obesity related metabolic dysregulation such that it can be selected as a therapeutic target to improve obesity related health problems. There are tools to over-express adiponectin such as drug induced secretion and osmotic pump drug delivery system for basic research. In this project, recombinant adeno-associated virus serotype 2/8 (rAAV2/8-LSP-mADN) was used to overexpress adiponectin in the liver of mouse for basic and translational research studies. Western blot of hepatocyte lysate and serum against adiponectin as well as hepatocyte viral quantitative PCR data suggests successful hepatic transduction. Although the expression level of adiponectin by this system need to further optimize, rAAV2/8-LSP-mADN is a valuable tool to over-express adiponectin in vivo safely and effectively.
DegreeMaster of Medical Sciences
SubjectAdipose tissues
Protein hormones
Adenoviruses
Dept/ProgramMedicine
Persistent Identifierhttp://hdl.handle.net/10722/221512

 

DC FieldValueLanguage
dc.contributor.authorChau, Chi-keung-
dc.contributor.author周志強-
dc.date.accessioned2015-11-26T23:38:31Z-
dc.date.available2015-11-26T23:38:31Z-
dc.date.issued2015-
dc.identifier.citationChau, C. [周志強]. (2015). Development of recombinant adeno-associated virus-mediated delivery adiponectin in mice. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5617458-
dc.identifier.urihttp://hdl.handle.net/10722/221512-
dc.description.abstractObesity is a global health problem experienced with various kinds of metabolic syndromes such as insulin resistance, hyperlipidemia and hypoadiponectinemia. To ameliorate obesity related problems, attention has been drawn to explore the role of adiponectin in obesity related metabolic dysregulation such that it can be selected as a therapeutic target to improve obesity related health problems. There are tools to over-express adiponectin such as drug induced secretion and osmotic pump drug delivery system for basic research. In this project, recombinant adeno-associated virus serotype 2/8 (rAAV2/8-LSP-mADN) was used to overexpress adiponectin in the liver of mouse for basic and translational research studies. Western blot of hepatocyte lysate and serum against adiponectin as well as hepatocyte viral quantitative PCR data suggests successful hepatic transduction. Although the expression level of adiponectin by this system need to further optimize, rAAV2/8-LSP-mADN is a valuable tool to over-express adiponectin in vivo safely and effectively.-
dc.languageeng-
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)-
dc.relation.ispartofHKU Theses Online (HKUTO)-
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.lcshAdipose tissues-
dc.subject.lcshProtein hormones-
dc.subject.lcshAdenoviruses-
dc.titleDevelopment of recombinant adeno-associated virus-mediated delivery adiponectin in mice-
dc.typePG_Thesis-
dc.identifier.hkulb5617458-
dc.description.thesisnameMaster of Medical Sciences-
dc.description.thesislevelMaster-
dc.description.thesisdisciplineMedicine-
dc.description.naturepublished_or_final_version-

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