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Article: Photoaffinity labeling of transcription factors by DNA-templated crosslinking

TitlePhotoaffinity labeling of transcription factors by DNA-templated crosslinking
Authors
Issue Date2015
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp
Citation
Chemical Science, 2015, v. 6 n. 1, p. 745-751 How to Cite?
AbstractCharacterization of transcription factor-DNA interaction is of high importance in elucidating the molecular mechanisms of gene transcriptions. DNA-based affinity probes were developed to capture and identify transcription factors by covalent crosslinking; however, the requirement of a crosslinker on the affinity probe remains a disadvantage, as the crosslinker itself often interferes with the protein-DNA interactions. We report a dual-probe method able to capture DNA-binding transcription factors with unmodified protein-binding sites in scenarios where conventional probes have failed. We have also shown the method's converse application in selecting specific transcription factor-binding DNA sequences from a probe library and its extension to studying proteins recognizing epigenetic marks. This study may provide a new tool for exploring DNA-binding proteins in biology. This journal is © The Royal Society of Chemistry.
Persistent Identifierhttp://hdl.handle.net/10722/221112
ISSN
2015 Impact Factor: 9.144
2015 SCImago Journal Rankings: 4.974
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZheng, W-
dc.contributor.authorZhang, W-
dc.contributor.authorChen, N-
dc.contributor.authorLiu, Y-
dc.contributor.authorLiu, Y-
dc.contributor.authorChen, L-
dc.contributor.authorZhou, X-
dc.contributor.authorChen, X-
dc.contributor.authorZheng, H-
dc.contributor.authorLi, X-
dc.date.accessioned2015-10-27T07:11:10Z-
dc.date.available2015-10-27T07:11:10Z-
dc.date.issued2015-
dc.identifier.citationChemical Science, 2015, v. 6 n. 1, p. 745-751-
dc.identifier.issn2041-6520-
dc.identifier.urihttp://hdl.handle.net/10722/221112-
dc.description.abstractCharacterization of transcription factor-DNA interaction is of high importance in elucidating the molecular mechanisms of gene transcriptions. DNA-based affinity probes were developed to capture and identify transcription factors by covalent crosslinking; however, the requirement of a crosslinker on the affinity probe remains a disadvantage, as the crosslinker itself often interferes with the protein-DNA interactions. We report a dual-probe method able to capture DNA-binding transcription factors with unmodified protein-binding sites in scenarios where conventional probes have failed. We have also shown the method's converse application in selecting specific transcription factor-binding DNA sequences from a probe library and its extension to studying proteins recognizing epigenetic marks. This study may provide a new tool for exploring DNA-binding proteins in biology. This journal is © The Royal Society of Chemistry.-
dc.languageeng-
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/publishing/journals/sc/About.asp-
dc.relation.ispartofChemical Science-
dc.titlePhotoaffinity labeling of transcription factors by DNA-templated crosslinking-
dc.typeArticle-
dc.identifier.emailLi, X: xiaoyuli@hku.hk-
dc.identifier.authorityLi, X=rp02080-
dc.identifier.doi10.1039/C4SC01953A-
dc.identifier.scopuseid_2-s2.0-84919344050-
dc.identifier.volume6-
dc.identifier.issue1-
dc.identifier.spage745-
dc.identifier.epage751-
dc.identifier.isiWOS:000345901600089-
dc.publisher.placeUnited Kingdom-

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