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Article: Quantitative And Qualitative Analysis Of The Extracellular Matrix Protein, Laminin, In Hirschsprung's Disease

TitleQuantitative And Qualitative Analysis Of The Extracellular Matrix Protein, Laminin, In Hirschsprung's Disease
Authors
Issue Date1992
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurg
Citation
Journal Of Pediatric Surgery, 1992, v. 27 n. 8, p. 991-996 How to Cite?
AbstractPrevious Immunohistochemical Studies Have Shown An Abnormal Distribution Of Extracellular Matrix (Ecm) Proteins, Including Laminin, In The Smooth Muscle Layer Of Muscularis Externa In Hirschsprung's Disease (Hd) Bowel. These Findings Supported The Hypothesis That An Abnormal Ecm Microenvironment May Be Responsible For The Failure Of Migration And/Or Development Of The Neural Crest Cells In The Gut In Hd. In Order To Determine The Cause Of The Abnormality In Laminin Distribution, Solid-Phase Enzyme-Linked Immunosorbent Assays And Immunoblots Were Used To Quantitate The Ecm Protein Laminin And Characterize Its Subunits, Respectively, In Extracts Of The Dissected Smooth Muscle Layer Of The Muscularis Externa. In The Aganglionic Bowel, Laminin (Median Concentration, 32.4 Ng/Mg Of Tissue) Was Found To Be Present In Significantly Greater Quantity Than In Both The Normoganglionic Bowel Of The Same Specimen (Median, 17.2 Ng/Mg, P ≤ .05) And The Normal Bowel Of Age- Matched Controls (Median, 9.7 Ng/Mg, P ≤ .05). Laminin Concentration Was Also Found To Be Significantly Higher In Normoganglionic Hd Bowel (Median, 17.2 Ng/Mg) Than In Age-Matched Control Specimens (Median, 10.8 Ng/Mg, P ≤ .05). No Difference Was Observed In The Subunit Composition Of Laminin In Hd And Control Extracts Analyzed By Immunoblot After Polyacrylamide Gel Electrophoresis. This Study Demonstrates A Quantitative Abnormality Of Laminin In The Bowel In Hd, Supporting The Hypothesis That 'Abnormal Microenvironment' May Have A Role In The Pathogenesis Of Hd.
Persistent Identifierhttp://hdl.handle.net/10722/220795
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.802
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorParikh, DHen_US
dc.contributor.authorTam, PKHen_US
dc.contributor.authorLloyd, DAen_US
dc.contributor.authorVan Velzen, Den_US
dc.contributor.authorEdgar, DHen_US
dc.date.accessioned2015-10-19T03:49:12Z-
dc.date.available2015-10-19T03:49:12Z-
dc.date.issued1992-
dc.identifier.citationJournal Of Pediatric Surgery, 1992, v. 27 n. 8, p. 991-996en_US
dc.identifier.issn0022-3468-
dc.identifier.urihttp://hdl.handle.net/10722/220795-
dc.description.abstractPrevious Immunohistochemical Studies Have Shown An Abnormal Distribution Of Extracellular Matrix (Ecm) Proteins, Including Laminin, In The Smooth Muscle Layer Of Muscularis Externa In Hirschsprung's Disease (Hd) Bowel. These Findings Supported The Hypothesis That An Abnormal Ecm Microenvironment May Be Responsible For The Failure Of Migration And/Or Development Of The Neural Crest Cells In The Gut In Hd. In Order To Determine The Cause Of The Abnormality In Laminin Distribution, Solid-Phase Enzyme-Linked Immunosorbent Assays And Immunoblots Were Used To Quantitate The Ecm Protein Laminin And Characterize Its Subunits, Respectively, In Extracts Of The Dissected Smooth Muscle Layer Of The Muscularis Externa. In The Aganglionic Bowel, Laminin (Median Concentration, 32.4 Ng/Mg Of Tissue) Was Found To Be Present In Significantly Greater Quantity Than In Both The Normoganglionic Bowel Of The Same Specimen (Median, 17.2 Ng/Mg, P ≤ .05) And The Normal Bowel Of Age- Matched Controls (Median, 9.7 Ng/Mg, P ≤ .05). Laminin Concentration Was Also Found To Be Significantly Higher In Normoganglionic Hd Bowel (Median, 17.2 Ng/Mg) Than In Age-Matched Control Specimens (Median, 10.8 Ng/Mg, P ≤ .05). No Difference Was Observed In The Subunit Composition Of Laminin In Hd And Control Extracts Analyzed By Immunoblot After Polyacrylamide Gel Electrophoresis. This Study Demonstrates A Quantitative Abnormality Of Laminin In The Bowel In Hd, Supporting The Hypothesis That 'Abnormal Microenvironment' May Have A Role In The Pathogenesis Of Hd.en_US
dc.languageengen_US
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurgen_US
dc.relation.ispartofJournal Of Pediatric Surgeryen_US
dc.titleQuantitative And Qualitative Analysis Of The Extracellular Matrix Protein, Laminin, In Hirschsprung's Diseaseen_US
dc.typeArticleen_US
dc.identifier.emailTam, PKH:paultam@hkucc.hku.hk-
dc.identifier.authorityTam, PKH=rp00060-
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1403563-
dc.identifier.scopuseid_2-s2.0-0026668553-
dc.identifier.volume27-
dc.identifier.issue8-
dc.identifier.spage991-
dc.identifier.epage996-
dc.identifier.isiWOS:A1992JF86500010-

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