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Article: Treatment With An Hla-Peptide And Cyclosporine A Prolongs Rat Small Bowel Allograft Survival

TitleTreatment With An Hla-Peptide And Cyclosporine A Prolongs Rat Small Bowel Allograft Survival
Authors
Issue Date1997
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurg
Citation
Journal Of Pediatric Surgery, 1997, v. 32 n. 3, p. 469-472 How to Cite?
AbstractBackground: The Ultimate Treatment For Severe Short Bowel Syndrome Is Small Bowel Transplantation (Sbt). Current Immunosuppression For Sbt Is Relatively Ineffective And Toxic. Peptides Derived From Residues 75-84 Of The Hla-B7 Molecule Are Immunomodulatory In Vitro, And In Rodents, When Combined With Subtherapeutic Doses Of Cyclosporine (Csa), Prolong Cardiac And Skin Allograft Survival Without Altering The Recipient's Rejection Kinetics To Third Party Allografts. We Investigated The Effects Of Hla-B7 Peptide Fragment In A Rat Model Of Sbt. Methods: Heterotopic Allogeneic Sbt Was Performed In Dagouti (Rt1a) To Lewis (Rt1(L)) High-Responder Rat Strain Combination. B7.75-84 (40 Mg/Kg/D) And Subtherapeutic Csa (10 Mg/Kg/D) Were Administered Alone, Or In Combination, By Gavage To Allograft Recipients On Days 0 To 4 After Sbt. Recipient Pretreatment With B7.75-84 On Days -14, - 12, -10, And -7 Followed By Subtherapeutic Csa On Days O To 4 After Sbt Was Also Carried Out. Graft Rejection Was Determined By The Presence Of A Palpable Abdominal Mass On Daily Examination Or By Loss Of More Than 10% Initial Body Weight. Results: Without Immunosuppression Allografts Rejected At A Median Time Of 6 Days (Range, 5 To 7; N = 7). This Was Not Significantly Altered With Either Csa Therapy Alone (Median 6 Days; Range, 6 To 7; N = 6) Or B7.75-84 Alone (Median, 5 Days; Range, 5 To 6; N = 6). Recipient Combination Therapy With B7.75-84 And Csa After Allografting Significantly Prolonged Allograft Survival (Median, 11 Days; Range, 9 To 13; N = 9), As Did Recipient B7.75-84 Pretransplant Therapy (Median, 10 Days; Range, 9 To 12; N = 6), When Administered Over A 2-Week Period Before Allografting. Conclusion: Post-Sbt Recipient Treatment With B7.75-84 Produced Statistically Significant Improvement In Allograft Survival Only After Combination With Subtherapeutic Csa. Recipient Pre-Sbt Treatment With B7.75-84 Alone However, Resulted In Statistically Significant Improvement In Allograft Survival In Combination With Post-Sbt Subtherapeutic Csa. These Synergistic Effects May Be Valuable In Achieving Improved Sbt Survival Clinically And Warrant Further Exploration.
Persistent Identifierhttp://hdl.handle.net/10722/220773
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.802
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWilletts, IEen_US
dc.contributor.authorTam, PKHen_US
dc.contributor.authorMorris, PJen_US
dc.contributor.authorDallman, MJen_US
dc.date.accessioned2015-10-19T01:33:10Z-
dc.date.available2015-10-19T01:33:10Z-
dc.date.issued1997-
dc.identifier.citationJournal Of Pediatric Surgery, 1997, v. 32 n. 3, p. 469-472en_US
dc.identifier.issn0022-3468-
dc.identifier.urihttp://hdl.handle.net/10722/220773-
dc.description.abstractBackground: The Ultimate Treatment For Severe Short Bowel Syndrome Is Small Bowel Transplantation (Sbt). Current Immunosuppression For Sbt Is Relatively Ineffective And Toxic. Peptides Derived From Residues 75-84 Of The Hla-B7 Molecule Are Immunomodulatory In Vitro, And In Rodents, When Combined With Subtherapeutic Doses Of Cyclosporine (Csa), Prolong Cardiac And Skin Allograft Survival Without Altering The Recipient's Rejection Kinetics To Third Party Allografts. We Investigated The Effects Of Hla-B7 Peptide Fragment In A Rat Model Of Sbt. Methods: Heterotopic Allogeneic Sbt Was Performed In Dagouti (Rt1a) To Lewis (Rt1(L)) High-Responder Rat Strain Combination. B7.75-84 (40 Mg/Kg/D) And Subtherapeutic Csa (10 Mg/Kg/D) Were Administered Alone, Or In Combination, By Gavage To Allograft Recipients On Days 0 To 4 After Sbt. Recipient Pretreatment With B7.75-84 On Days -14, - 12, -10, And -7 Followed By Subtherapeutic Csa On Days O To 4 After Sbt Was Also Carried Out. Graft Rejection Was Determined By The Presence Of A Palpable Abdominal Mass On Daily Examination Or By Loss Of More Than 10% Initial Body Weight. Results: Without Immunosuppression Allografts Rejected At A Median Time Of 6 Days (Range, 5 To 7; N = 7). This Was Not Significantly Altered With Either Csa Therapy Alone (Median 6 Days; Range, 6 To 7; N = 6) Or B7.75-84 Alone (Median, 5 Days; Range, 5 To 6; N = 6). Recipient Combination Therapy With B7.75-84 And Csa After Allografting Significantly Prolonged Allograft Survival (Median, 11 Days; Range, 9 To 13; N = 9), As Did Recipient B7.75-84 Pretransplant Therapy (Median, 10 Days; Range, 9 To 12; N = 6), When Administered Over A 2-Week Period Before Allografting. Conclusion: Post-Sbt Recipient Treatment With B7.75-84 Produced Statistically Significant Improvement In Allograft Survival Only After Combination With Subtherapeutic Csa. Recipient Pre-Sbt Treatment With B7.75-84 Alone However, Resulted In Statistically Significant Improvement In Allograft Survival In Combination With Post-Sbt Subtherapeutic Csa. These Synergistic Effects May Be Valuable In Achieving Improved Sbt Survival Clinically And Warrant Further Exploration.en_US
dc.languageengen_US
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurgen_US
dc.relation.ispartofJournal Of Pediatric Surgeryen_US
dc.titleTreatment With An Hla-Peptide And Cyclosporine A Prolongs Rat Small Bowel Allograft Survivalen_US
dc.typeArticleen_US
dc.identifier.emailTam, PKH:paultam@hkucc.hku.hk-
dc.identifier.authorityTam, PKH=rp00060-
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0022-3468(97)90608-4-
dc.identifier.pmid9094020-
dc.identifier.scopuseid_2-s2.0-0030898890-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030898890&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume32-
dc.identifier.issue3-
dc.identifier.spage469-
dc.identifier.epage472-
dc.identifier.isiWOS:A1997WP14100023-

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